Carmoisine
(Synonyms: 偶氮玉红; Azorubine; Acid Red 14; E122) 目录号 : GC60671
Azo Rubine is an synthetic red food dye and is used for the purposes where food is heat-treated after fermentation.
Cas No.:3567-69-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Azo Rubine is an synthetic red food dye and is used for the purposes where food is heat-treated after fermentation.
| Cas No. | 3567-69-9 | SDF | |
| 别名 | 偶氮玉红; Azorubine; Acid Red 14; E122 | ||
| Canonical SMILES | O=S(C1=C2C=CC=CC2=C(O)C(/N=N/C3=C4C=CC=CC4=C(S(=O)(O[Na])=O)C=C3)=C1)(O[Na])=O | ||
| 分子式 | C20H12N2Na2O7S2 | 分子量 | 502.43 |
| 溶解度 | DMSO: 50 mg/mL (99.52 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9903 mL | 9.9516 mL | 19.9033 mL |
| 5 mM | 0.3981 mL | 1.9903 mL | 3.9807 mL |
| 10 mM | 0.199 mL | 0.9952 mL | 1.9903 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Advancement in electrochemical strategies for quantification of Brown HT and Carmoisine (Acid Red 14) From Azo Dyestuff class
Food Chem Toxicol 2022 Jul;165:113075.PMID:35487338DOI:10.1016/j.fct.2022.113075.
Brown HT and Carmoisine, which are the most used dyestuffs in pharmaceuticals, textiles, cosmetics and foods, are important components of the Azo family. Although the Azo group is not toxic or carcinogenic under normal conditions, these dyestuffs require great care due to the reduction of the Azo functional group to amines. In particular, fast, reliable, easy, on-site and precise determinations of these substances are extremely necessary and important. In this review, the properties, applications, and electrochemical determinations of brown HT and Carmoisine, which are used as synthetic food colorants, are discussed in detail. Up to now, sensor types, detection limits (LOD and LOQ), and analytical applications in the developed electrochemical strategies for both substances were compared. In addition, the validation parameters such as the variety of the sensors, sensitivity, selectivity and electrochemical technique in these studies were clarified one by one. While the electrochemical techniques recommended for brown HT were mostly used for the removal of dyestuff, for Carmoisine they included fully quantitative centered studies. The percentiles of voltammetric techniques, which are the most widely used among these electroanalytical methods, were determined. The benefits of a robust electrochemical strategy for the determination of both food colors are summed up in this review. Finally, the brown HT and Carmoisine suggestions for future perspectives in electrochemical strategy are given according to all their applications.
Simultaneous detection of Carmoisine and tartrazine in food samples using GO-Fe3O4-PAMAM and ionic liquid based electrochemical sensor
Food Chem Toxicol 2022 Feb 12;162:112864.PMID:35157927DOI:10.1016/j.fct.2022.112864.
This study was performed to investigate the simultaneous detection of Carmoisine and tartrazine, two food azo dyes, with a new voltammetric sensor based on graphene oxide-Fe3O4 (GO-Fe3O4) nanocomposite functionalized with fourth-generation poly(amidoamine) (G4 PAMAM) dendrimers (GO-Fe3O4-G4 PAMAM) and ionic liquid (IL) modified carbon paste electrode (GO-Fe3O4-G4 PAMAM/ILCPE). The GO-Fe3O4-G4 PAMAM was synthesized and characterized by X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), vibrating-sample magnetometer (VSM), and fourier transform infrared (FT-IR) techniques. Cyclic voltammetry (CV) was used to evaluate the electrochemical behavior of Carmoisine, revealing the good electrocatalytic function of GO-Fe3O4-G4 PAMAM/ILCPE. Linear response from 0.1 to 170.0 μM was obtained based on Carmoisine electrochemical oxidation through differential pulse voltammetry (DPV). The limit of detection (LOD) value obtained was 0.02 μM. Also, the GO-Fe3O4-G4 PAMAM/ILCPE was used for the simultaneous determination of Carmoisine and tartrazine. In co-existence system containing Carmoisine and tartrazine, the developed sensor exhibited well-defined and separate DPV peaks (i.e., 770 mV) for Carmoisine and tartrazine. Besides, repeatability, reproducibility and stability studies were performed. Additionally, the analytical application of this sensor was demonstrated by determination of Carmoisine and tartrazine in food samples including lemon juice and powdered juice.
Toxicity effect of the edible pigment Carmoisine on Polyrhachis vicina Roger (Hymenoptera: Formicidae)
Ecotoxicology 2022 Aug;31(6):1009-1022.PMID:35792963DOI:10.1007/s10646-022-02563-1.
Carmoisine belongs to a water-soluble synthetic dye and is often used as a food additive. Previous research has shown that Carmoisine is toxic to rats and zebrafish, but there have been few reports on the effect of Carmoisine on soil-dwelling social insects. The present study evaluated Carmoisine toxicity in Polyrhachis vicina Roger. We found that the effects of different concentrations of Carmoisine on the mortality of workers were dose-dependent. The 10% lethal dose (LD10), 50% lethal dose (LD50) and 90% lethal dose (LD90) of Carmoisine to workers at 96 h was calculated to be 0.504, 5.491 and 10.478 g/L, respectivily. LD10 of workers were selected to treat the fourth instar larvae, pupae and adults for 10 days. The results showed that the survival rate of all ants, except for females, was significantly reduced, especially larvae and workers. The body weight of larvae, pupae and males decreased significantly, while weight gain was observed in the females and workers. The appearance of larvae, pupae and workers changed after Carmoisine treatment, such as body darkening and epidermis shrinking of larvae and pupae, as well as body segment expansion of workers. Furthermore, Carmoisine altered the expression of the estrogen-related receptor, tailless and homothorax of P. vicina (Pv-ERR, Pv-tll and Pv-hth) to varying degrees in larvae and adults. We believe that variations in body weight can lead to a decrease in survival rate and appearance changes in the ants, which may be related to abnormal gene expressions caused by Carmoisine treatment. Therefore, we confirm that Carmoisine has negative effects on the growth and development of P. vicina.
Effects of the food colorant Carmoisine on zebrafish embryos at a wide range of concentrations
Arch Toxicol 2022 Apr;96(4):1089-1099.PMID:35146542DOI:10.1007/s00204-022-03240-2.
Since the middle of the twentieth century, the use of dyes has become more common in every food group as well as in the pharmaceutical, textile and cosmetic industries. Azo dyes, including Carmoisine, are the most important of the dye classes with the widest color range. In this study, the effects of Carmoisine exposure on the embryonic development of zebrafish at a wide dose scale, including recommended and overexposure doses (from 4 to 2000 ppm), were investigated in detail. For this purpose, many morphological and physiological parameters were examined in zebrafish exposed to Carmoisine at determined doses for 96 h, and the mechanisms of action of the changes in these parameters were tried to be clarified with the metabolite levels determined. The no observed effect concentration (NOEC) and median lethal concentration (LC50) were recorded at 5 ppm and 1230.53 ppm dose at 96 hpf, respectively. As a result, it was determined that the applied Carmoisine caused serious malformations, reduction in height and eye diameter, increase in the number of free oxygen radicals, in apoptotic cells and in lipid accumulation, decrease in locomotor activity depending on the dose and at the highest dose, decrease in blood flow rate. In the metabolome analysis performed to elucidate the metabolism underlying all these changes, 45 annotated metabolites were detected.
Biological activated carbon process for biotransformation of azo dye Carmoisine by Klebsiella spp
Environ Technol 2022 Jul;43(18):2713-2729.PMID:33641622DOI:10.1080/09593330.2021.1897167.
The feasibility of employing the biological activated carbon (BAC) process to debilitate azo dye Carmoisine by Klebsiella spp. was investigated. Plate assay revealed the capability of Klebsiella spp. for removal of Carmoisine via degradation. Kinetic parameters were measured for Carmoisine debilitation by Klebsiella spp. using the suspended anaerobic process. Two types of granular and rod-shaped activated carbon were used to form the biological beds in order to study the Carmoisine debilitation in batch processes. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to indicate the colonization and biofilm formation of bacteria grown on activated carbon particles (ACPs). Thin-layer chromatography (TLC), liquid chromatography-mass spectrometry (LC-MS), high-pressure liquid chromatography (HPLC) and biosorption studies demonstrated biotransformation of Carmoisine into its constituent aromatic amines during the Carmoisine debilitation in suspended anaerobic and BAC processes. The porosity of activated carbons, inoculation size and age of biological beds were the important factors affecting the viability of bacterial cells grown on ACPs and, consequently, the rate and efficiency of the Carmoisine debilitation process determined through spectrophotometry. The reusability of biological beds was demonstrated by conducting sequential batch experiments. In conclusion, the BAC process proved to be an efficient method for anaerobic dye degradation.