c-di-AMP
(Synonyms: 环二腺苷酸; Cyclic diadenylate; Cyclic-di-AMP) 目录号 : GC13643
C-di-AMP is a STING agonist, which binds to the transmembrane protein STING, thereby activating the TBK3-IRF3 signaling pathway, subsequently triggering the production of type I IFN and TNF.
Cas No.:54447-84-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Bone marrow- derived DC(BMDC) |
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Preparation Method |
BMDCs were stimulated with irradiated tumor-supernatants, or c-di-AMP (5 ug/ml) or a combination for 48 h on day 7. |
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Reaction Conditions |
5 ug/ml;48h |
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Applications |
Combination of c-di-AMP with radiation promotes the secretion of type I IFN and ISG levels. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6 male mice and cGAS KO (Mb21d1-/-) mice |
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Preparation Method |
Mice were injected c-di-AMP intraperitoneally with 25ug one day before radiotherapy, and once every other day, a total of three times. |
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Dosage form |
25ug;i.p;3 times |
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Applications |
C-di-AMP acts as STING agonist, cooperated with dsDNA to promote DC maturation and type I IFN release. |
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References: [1]. Li Z, Zhang Y, et,al. Gut microbiota modulate radiotherapy-associated antitumor immune responses against hepatocellular carcinoma Via STING signaling. Gut Microbes. 2022 Jan-Dec;14(1):2119055. doi: 10.1080/19490976.2022.2119055. PMID: 36093568; PMCID: PMC9467592. |
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C-di-AMP is a STING agonist, which binds to the transmembrane protein STING, thereby activating the TBK3-IRF3 signaling pathway, subsequently triggering the production of type I IFN and TNF. c-di-AMP serves as a second messenger in bacteria, primarily regulating cell growth, survival, and virulence in Gram-positive bacteria, and also modulates the host immune response. As an effective mucosal adjuvant, c-di-AMP stimulates both humoral and cellular responses[1-5]. C-di-AMP have been utilized as vaccine adjuvants for influenza and hepatitis C.Citation[6].
Combination of c-di-AMP(5 ug/ml;48h) with radiation promotes the secretion of type I IFN and ISG levels in BMDCs[7].
C-di-AMP(25ug;i.p;3 times) can synergistically enhance the antitumor effects of Radiotherapy (RT)-associated dsDNA by promoting IFN-β production and CD8+ cytolytic T cell activation in a cGAS- and STING-dependent manner in mice[7].
C-di-AMP是STING(刺激内质网的蛋白)的激动剂,通过与该跨膜蛋白结合,激活TBK3-IRF3信号通路,进而引发I型IFN和TNF的产生。c-di-AMP在细菌中作为第二信使发挥作用,主要调节革兰氏阳性菌的细胞生长、存活和毒力,并调节宿主的免疫反应。作为有效的粘膜佐剂,c-di-AMP可以刺激体液和细胞反应[1-5]。C-di-AMP已被用作流感和丙型肝炎的疫苗佐剂[6]。
C-di-AMP(5 ug/ml;48h)联合放疗可促进BMDCs分泌I型IFN和ISG[7]。
C-di-AMP(25ug;i.p;3 times) 可以协同增强RT相关dsDNA的抗肿瘤作用,通过cGAS-和STING-依赖的方式促进小鼠IFN-β的产生和CD8+细胞溶解T细胞的活化[7]。
References:
[1]. Jenal U, Reinders A, et,al.Cyclic di-GMP: second messenger extraordinaire. Nat Rev Microbiol. 2017 May;15(5):271-284. doi: 10.1038/nrmicro.2016.190. Epub 2017 Feb 6. PMID: 28163311.
[2]. Fahmi T, Port GC, et,al.c-di-AMP: An Essential Molecule in the Signaling Pathways that Regulate the Viability and Virulence of Gram-Positive Bacteria. Genes (Basel). 2017 Aug 7;8(8):197. doi: 10.3390/genes8080197. PMID: 28783096; PMCID: PMC5575661.
[3]. Ning H, Wang L, et,al. Recombinant BCG With Bacterial Signaling Molecule Cyclic di-AMP as Endogenous Adjuvant Induces Elevated Immune Responses After Mycobacterium tuberculosis Infection. Front Immunol. 2019 Jul 3;10:1519. doi: 10.3389/fimmu.2019.01519. PMID: 31333655; PMCID: PMC6618344.
[4]. Ebensen T, Delandre S, et,al. The Combination Vaccine Adjuvant System Alum/c-di-AMP Results in Quantitative and Qualitative Enhanced Immune Responses Post Immunization. Front Cell Infect Microbiol. 2019 Feb 19;9:31. doi: 10.3389/fcimb.2019.00031. PMID: 30838180; PMCID: PMC6390046.
[5]. Sanchez MV, Ebensen T, et,al. Intranasal delivery of influenza rNP adjuvanted with c-di-AMP induces strong humoral and cellular immune responses and provides protection against virus challenge. PLoS One. 2014 Aug 20;9(8):e104824. doi: 10.1371/journal.pone.0104824. PMID: 25140692; PMCID: PMC4139298.
[6]. Yin W, Cai X, et,al. A decade of research on the second messenger c-di-AMP. FEMS Microbiol Rev. 2020 Nov 24;44(6):701-724. doi: 10.1093/femsre/fuaa019. PMID: 32472931; PMCID: PMC7850090.
[7]. Li Z, Zhang Y, et,al. Gut microbiota modulate radiotherapy-associated antitumor immune responses against hepatocellular carcinoma Via STING signaling. Gut Microbes. 2022 Jan-Dec;14(1):2119055. doi: 10.1080/19490976.2022.2119055. PMID: 36093568; PMCID: PMC9467592.
| Cas No. | 54447-84-6 | SDF | |
| 别名 | 环二腺苷酸; Cyclic diadenylate; Cyclic-di-AMP | ||
| 化学名 | (2R,3S,3aS,7aS,9R,10aS,14aS)-2,9-bis(6-amino-9H-purin-9-yl)-3,5,10,12-tetrahydroxydecahydrodifuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine 5,12-dioxide | ||
| Canonical SMILES | NC1=C2C(N([C@@H]3O[C@@]([H])(COP4(O)=O)[C@@]([H])(OP(OC[C@@]5([H])[C@]([H])([C@H](O)[C@H](N6C7=NC=NC(N)=C7N=C6)O5)O4)(O)=O)C3O)C=N2)=NC=N1 | ||
| 分子式 | C20H24N10O12P2 | 分子量 | 658.41 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5188 mL | 7.5941 mL | 15.1881 mL |
| 5 mM | 0.3038 mL | 1.5188 mL | 3.0376 mL |
| 10 mM | 0.1519 mL | 0.7594 mL | 1.5188 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。