Bromocresol green
目录号 : GC20126
Cas No.:76-60-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cas No. | 76-60-8 | SDF | |
| 分子式 | C21H14Br4O5S | 分子量 | 698.01 |
| 溶解度 | Easily soluble in ethanol, ether and ethyl acetate, soluble in benzene, slightly soluble in water. | 储存条件 | RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4326 mL | 7.1632 mL | 14.3264 mL |
| 5 mM | 0.2865 mL | 1.4326 mL | 2.8653 mL |
| 10 mM | 0.1433 mL | 0.7163 mL | 1.4326 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Binding of Bromocresol green and bromocresol purple to albumin in hemodialysis patients
Clin Chem Lab Med 2018 Feb 23;56(3):436-440.PMID:28985181DOI:10.1515/cclm-2017-0444.
Background: Colorimetric albumin assays based on binding to bromocresol purple (BCP) and Bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin. Methods: Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated. Results: Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V. Conclusions: In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.
Removal of Bromocresol green from aqueous solution by electro-Fenton and electro-Fenton-like processes with different catalysts: laboratory and kinetic model investigation
Water Sci Technol 2021 Nov;84(10-11):3227-3236.PMID:34850723DOI:10.2166/wst.2021.407.
This study presents the removal of triarylmethane dye Bromocresol green from aqueous solution by the electro-Fenton process. As catalysts five different cations were used: Fe2+, Ce3+, Ni2+, Mn2+, and Co2+. They play crucial roles in the whole process because they react with H2O2 producing hydroxyl radicals that are capable of breaking down dye molecules. Based on this, a comparison of catalytic activity of these cations in the electro-Fenton process is made for Bromocresol green degradation. A simple and universal kinetic model is also applied to study the catalytic activity of investigated catalysts. Due to its multidimensionality it is fitted to experimental data using a genetic algorithm. The procedure of fitting using a genetic algorithm is thoroughly described and demonstrated. The activity of utilized catalysts is compared based on both experimental and model data revealing that for Bromocresol green removal all alternative catalysts (Ni2+, Co2+, Ce3+, Mn2+) are better than the typical one (Fe2+, 51.83% degradation). The best catalyst is Co2+ with 78.35% degradation efficiency. Moreover, the adopted kinetic model proved its universality and outlined different interactions between catalysts and dye molecules.
Preparation of Chitosan Poly(methacrylate) Composites for Adsorption of Bromocresol green
ACS Omega 2019 Jul 25;4(7):12680-12686.PMID:31460389DOI:10.1021/acsomega.9b01576.
In the present study, chitosan poly(methacrylate) composites were prepared and applied for adsorption of Bromocresol green from aqueous solutions. The synthesized composites were characterized with scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The Bromocresol green removal by the developed adsorbent was investigated, and the effects of experimental parameters, including sample pH and adsorption time, were also examined. Furthermore, the adsorption characteristics of the synthesized adsorbent, including kinetics, adsorption isotherms, and thermodynamics, were comprehensively studied. The adsorption isotherm was well described by the Freundlich model, and the maximum adsorption capacity was 39.84 μg mg-1 by shaking for 40 min at pH 2.0. Bromocresol green adsorption kinetics followed a pseudo-second-order kinetic model, indicating that adsorption was the rate-limiting step. Thermodynamic parameters and the negative values of Gibbs free energy change (ΔG°) showed that adsorption was a spontaneous process. The positive values of entropy change (ΔS°) implied that the adsorption of Bromocresol green on chitosan poly(methacrylate) composites was an increasing random process. In addition, enthalpy change (ΔH°) values were positive, suggesting that the adsorption of Bromocresol green was endothermic. The adsorption percentage of Bromocresol green with chitosan poly(methacrylate) composites remained above 97% after three times of recycling test.
Serum albumin by dye-binding: Bromocresol green or bromocresol purple? The case for conservatism
Ann Clin Biochem 1988 Jul;25 ( Pt 4):417-21.PMID:3214125DOI:10.1177/000456328802500417.
Pooled patient's serum selected to have a wide range of albumin concentrations was analysed for albumin by Bromocresol green with both long and short incubation times and also by bromocresol purple. Total protein, colloid osmotic pressure, calcium and magnesium were also measured. There were strong linear correlations between albumin measured by the three methods. Albumin values by Bromocresol green with a short incubation time (1.5 min) averaged 5 g/L higher than those by bromocresol purple at all albumin concentrations. Colloid osmotic pressure correlated less strongly with total protein and with albumin by bromocresol purple than with albumin by the two Bromocresol green methods. There were no significant differences between the correlation coefficients of calcium or magnesium with total protein and with albumin measured by the three methods. Bromocresol purple has no advantage over Bromocresol green with a short incubation time for the clinical purposes for which albumin is measured: to detect abnormality, monitor change, predict colloid osmotic pressure and adjust calcium and magnesium for abnormal protein concentrations.
Poly (Bromocresol green) flakes-decorated pencil graphite electrode for selective electrochemical sensing applications and pharmacokinetic studies
Mater Sci Eng C Mater Biol Appl 2019 Sep;102:634-645.PMID:31147035DOI:10.1016/j.msec.2019.03.071.
A square wave voltammetric method for selective determination of meropenem (MRP) and ertapenem (ERP) was developed using pencil graphite electrode modified with poly (Bromocresol green) (PGE/PBCG). The modified electrode film was characterized by scanning electron microscopy and electro-chemical impedance spectroscopy. Under the optimized conditions, the prepared electrode has good linearity over concentration range 1.0-60.0 and 0.3.0-75.0 μM for MRP and ERP, respectively. The developed method was validated according to ICH guidelines. In addition, the diffusion co-efficients of MRP and ERP were estimated to be 1.24 × 10-6 and 9.09 × 10-6 cm2 s-1, respectively using chronoamperometric technique. The developed method was highly sensitive and selective for the determination of MRP or ERP in the presence of their corresponding open beta-lactam ring degradation products. Consequently, it was successfully utilized for in-vitro and in-vivo applications in spiked and real plasma samples of healthy rabbits for their pharmacokinetic studies. Furthermore, the method was applied for the assay of the available dosage forms of both drugs.