Bisphenol A
(Synonyms: 双酚A) 目录号 : GC39480
Bisphenol A是一种酚类有机合成化合物，是合成塑料（主要是某些聚碳酸酯和环氧树脂）、聚砜的原料。
Cas No.:80-05-7
Sample solution is provided at 25 µL, 10mM.
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Bisphenol A is a phenolic organic synthetic compound, which is used as a raw material for synthetic plastics (mainly certain polycarbonates and epoxy resins), and polysulfones [1]. Bisphenol A can be used in studies related to cancer, cardiovascular diseases, respiratory diseases, diabetes, kidney diseases, obesity, and reproductive disorders [2].
In vitro, Bisphenol A (0-400μM, 24 hours) inhibited the proliferation of human colon cancer cells HCT116 and induced their apoptosis through the mitochondrial and MAPK/AKT pathways [3]. Bisphenol A (0.5-12.5μM, 5 days) dose-dependently inhibited the formation of TRAP-positive multinucleated cells induced by RANKL in bone marrow-derived macrophages (BMM) [4].
In vivo, Bisphenol A (200mg/kg/d; SC; 4 weeks) significantly reduced the weights of the testes, epididymis, prostate and seminal vesicles in Wistar rats, as well as the daily sperm production in the testes [5]. Bisphenol A (100µg/kg; twice a day for 8 days; SC) inhibited the basal insulin secretion in mice, reduced food intake, body temperature and activity ability of mice, and upregulated the expression of IRS-1 protein under basal conditions in skeletal muscle [6].
References:
[1] Rubin BS. Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol. 2011 Oct;127(1-2):27-34.
[2] Rochester J R. Bisphenol A and human health: a review of the literature[J]. Reproductive toxicology, 2013, 42: 132-155.
[3] Qu W, Zhao Z, Chen S, Zhang L, Wu D, Chen Z. Bisphenol A suppresses proliferation and induces apoptosis in colonic epithelial cells through mitochondrial and MAPK/AKT pathways. Life Sci. 2018 Sep 1;208:167-174.
[4] Hwang JK, Min KH, Choi KH, Hwang YC, Jeong IK, Ahn KJ, Chung HY, Chang JS. Bisphenol A reduces differentiation and stimulates apoptosis of osteoclasts and osteoblasts. Life Sci. 2013 Sep 17;93(9-11):367-72.
[5] Takahashi O, Oishi S. Testicular toxicity of dietarily or parenterally administered bisphenol A in rats and mice. Food Chem Toxicol. 2003 Jul;41(7):1035-44.
[6] Batista T M, Alonso-Magdalena P, Vieira E, et al. Short-term treatment with bisphenol-A leads to metabolic abnormalities in adult male mice[J]. PloS one, 2012, 7(3): e33814.
Bisphenol A是一种酚类有机合成化合物，是合成塑料（主要是某些聚碳酸酯和环氧树脂）、聚砜的原料 [1]。Bisphenol A可用于癌症、心血管疾病、呼吸系统疾病、糖尿病、肾脏疾病、肥胖和生殖障碍的研究 [2]。
在体外，Bisphenol A（0-400μM；24h）能够通过线粒体和MAPK/AKT通路抑制人结肠癌细胞HCT116的增殖并诱导其凋亡 [3]。Bisphenol A（0.5-12.5μM；5 天）能够剂量依赖性抑制骨髓来源的巨噬细胞（BMM）中RANKL诱导的TRAP阳性多核细胞形成 [4]。
在体内，Bisphenol A（200mg/kg/d；SC；4 weeks）显著降低Wistar大鼠的睾丸、附睾、前列腺和精囊重量，以及睾丸每日精子产量 [5]。Bisphenol A（100µg/kg；twice a day for 8 days；SC）抑制了小鼠的基础胰岛素分泌，降低了小鼠食物摄入量、体温降低和活动能力，并且上调IRS-1蛋白在骨骼肌的基础条件下的表达 [6] 。
| Cell experiment [1]: | |
Cell lines | RAW 264.7 cells |
Preparation Method | Cell viability assay was carried out with the MTT assay. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction was also used to assess cell viability. Briefly, RAW 264.7 cells (1.0×104 cells/wells) were seeded in a 96 well plate with α-MEM containing 10% FBS for 24h. Cells were added with various concentrations of Bisphenol A for 24h, washed with PBS three times and treated with medium containing 100μg/ml of MTT for 2h at 37 °C, washed with PBS, and then solubilized in DMSO. The resulting intracellular purple formazan was quantified with a spectrophotometer by measuring the absorbance at 570nm. |
Reaction Conditions | 0.5, 2.5 and 12.5μM; 24h |
Applications | Cell viability significantly reduced to 80% with 12.5μM of Bisphenol A, but a lower dosage of BPA did not decreased cell viability. |
| Animal experiment [2]: | |
Animal models | Swiss albino OF1 mice |
Preparation Method | Experiments were performed with 3 months old male Swiss albino OF1 mice that were individually maintained under standard housing conditions. BPA was dissolved in tocopherol-stripped corn oil and administered subcutaneously during 8 days twice a day (9:00a.m. and 2:00p.m.). Control mice were injected with 100µl of vehicle at the same time-points. The total daily dose used for Bisphenol A was 100µg/kg. To measure plasma metabolites blood samples were collected from the tail vein. Insulin was measured by enzyme-linked immunosorbent assay and NEFA was measured using a commercial kit. |
Dosage form | 100µg/kg; twice a day for 8 days; SC |
Applications | Bisphenol A inhibited the basal insulin secretion in mice, reduced their food intake, decreased their body temperature and impaired their activity. Moreover, it upregulated the expression of IRS-1 protein under basal conditions in skeletal muscle. |
References: | |
| Cas No. | 80-05-7 | SDF | |
| 别名 | 双酚A | ||
| Canonical SMILES | CC(C1=CC=C(O)C=C1)(C2=CC=C(O)C=C2)C | ||
| 分子式 | C15H16O2 | 分子量 | 228.29 |
| 溶解度 | DMSO: 100 mg/mL (438.04 mM); Water: < 0.1 mg/mL (insoluble) | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.3804 mL | 21.902 mL | 43.8039 mL |
| 5 mM | 0.8761 mL | 4.3804 mL | 8.7608 mL |
| 10 mM | 0.438 mL | 2.1902 mL | 4.3804 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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