BGLAP Protein, Mouse (His-SUMO)
目录号 : GC26916BGLAP 蛋白在其羧化状态下形成骨基质的重要组成部分并充当骨形成的负调节剂。它限制骨形成并保留吸收和矿化等过程。BGLAP 蛋白, Mouse (His-SUMO) 是重组的 BGLAP 蛋白，由 E. coli 表达，带有 N-6*His, N-SUMO 标签。
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
BGLAP 蛋白在其羧化状态下形成骨基质的重要组成部分并充当骨形成的负调节剂。它限制骨形成并保留吸收和矿化等过程。BGLAP 蛋白, Mouse (His-SUMO) 是重组的 BGLAP 蛋白，由 E. coli 表达，带有 N-6*His, N-SUMO 标签。
羧化形式的 BGLAP 蛋白是骨基质中的主要有机成分，占总骨蛋白的 1-2%。在这种羧化状态下，它作为骨形成的负调节剂，对于限制骨形成同时保持骨吸收和矿化过程至关重要。值得注意的是，羧化形式对磷灰石和钙表现出很强的结合亲和力。相反，未羧化的形式充当成骨细胞分泌的激素，对多种细胞过程发挥调节影响。这包括它在能量代谢中的作用，它作为一种激素促进胰腺β细胞增殖、胰岛素分泌、胰岛素敏感性和能量消耗。此外，未羧化的骨钙素激素通过与 G 蛋白偶联受体 GPRC6A 相互作用促进睾丸中睾酮的产生，从而在男性生育能力中发挥关键作用。此外，它还充当大脑发育的调节剂，穿过血脑屏障启动信号反应，防止海马神经元凋亡，刺激单胺神经递质的合成，并抑制γ-氨基丁酸（GABA）的合成。重要的是，母体骨钙素在怀孕期间穿过胎盘，在胎儿大脑发育中起着至关重要的作用。
| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | E. coli |
| Phycical Appearance | Lyophilized powder | Shipping Condition | |
| Synonyms | BGLAP 蛋白, Mouse (His-SUMO); Bglap; Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein | ||
| Amino Acid Sequence | YLGASVPSPD PLEPTREQCE LNPACDELSD QYGLKTAYKR IYGITI | ||
| Apparent Molecular Weight | Approximately 24 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0. | ||
The BGLAP protein, in its carboxylated form, stands as a major organic constituent within the bone matrix, representing 1-2% of the total bone protein. In this carboxylated state, it serves as a negative regulator of bone formation, crucial for limiting bone formation while preserving the processes of bone resorption and mineralization. Notably, the carboxylated form exhibits strong binding affinity to apatite and calcium. Conversely, the uncarboxylated form functions as a hormone secreted by osteoblasts, exerting regulatory influence over diverse cellular processes. This includes its role in energy metabolism, where it acts as a hormone promoting pancreatic beta-cell proliferation, insulin secretion, insulin sensitivity, and energy expenditure. Moreover, the uncarboxylated osteocalcin hormone functions as a key player in male fertility by promoting testosterone production in the testes through its interaction with the G protein-coupled receptor GPRC6A. Additionally, it acts as a regulator of brain development, crossing the blood-brain barrier to initiate signaling responses that prevent neuronal apoptosis in the hippocampus, stimulate the synthesis of monoamine neurotransmitters, and inhibit gamma-aminobutyric acid (GABA) synthesis. Importantly, maternal osteocalcin, which crosses the placenta during pregnancy, plays a crucial role in fetal brain development.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.