beta-NGF Protein, Rat (HEK293, His)
目录号 : GC27028神经生长因子 (NGF) 是调节神经元存活和分化的神经营养因子，也是一种精浆蛋白，参与促排卵和促黄体作用。NGF 在女性生殖系统中具有潜在功能，有助于克服当前早期胚胎丢失的问题。
Sample solution is provided at 25 µL, 10mM.
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神经生长因子 (NGF) 是调节神经元存活和分化的神经营养因子，也是一种精浆蛋白，参与促排卵和促黄体作用。NGF 在女性生殖系统中具有潜在功能，有助于克服当前早期胚胎丢失的问题。
神经生长因子-β（Beta-NGF；NGF）是一种由 118 个氨基酸组成的碱性蛋白，作为周围神经系统的感觉和交感神经元以及前基底大脑的胆碱能神经元的营养因子。NGF 参与神经元存活和分化的调节。NGF 水平升高与创伤后应激障碍 (PTSD) 的风险相关。创伤反应导致负责 NGF 表达的 DNA 核苷酸甲基化。NGF 水平显示交感纤维密度的增加与 NGF 信使 RNA (mRNA) 水平成正比。NGF也是哺乳动物中常见的精浆蛋白。例如，NGF 作为骆驼的排卵刺激因子，并已被证明对公牛具有促黄体生成作用。NGF 在女性生殖系统中具有潜在的功能。例如，NGF在排卵诱导、LH释放、排卵、黄体发育、黄体酮（P4）产生、黄体体血管化和促性腺激素反应中发挥重要作用。对牛施用 NGF 可通过增加 LH 释放来增强类固醇产生、黄体形成和功能，并导致下游妊娠和发育标志物 mRNA 表达增加。此外，NGF 潜在的黄体生成作用有助于克服目前早期胚胎丢失的问题。人和牛NGF蛋白序列相似度为90.87%。同时，人NGF与大鼠和小鼠的相似性分别为85.89%和85.06%。
| Purity | Greater than 90% as determined by reducing SDS-PAGE | Source | HEK293 |
| Phycical Appearance | Lyophilized powder | Shipping Condition | |
| Synonyms | beta-NGF 蛋白; Beta-nerve growth factor; Ngf; Beta-NGF; Ngfb; beta Nerve Growth Factor | ||
| Apparent Molecular Weight | Approximately 15 kDa & 42 kDa, based on SDS-PAGE under reducing conditions. | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 1.463 ng/mL, corresponding to a specific activity is 6.835×105 units/mg. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4. | ||
Nerve Growth Factor-β (Beta-NGF; NGF) is a neurotrophic factor that regulates neuronal survival and differentiation. NGF is also a seminal plasma protein involved in ovulation and luteinizing. NGF has potential functions in the female reproductive system to help overcome the current problem of early embryo loss. Nerve Growth Factor-β (Beta-NGF; NGF) is a basic protein of 118 amino acids which acts are a trophic factor for sensory and sympathetic neurons of the peripheral nervous system, and on cholinergic neurons of the anterior basal cerebrum. NGF involves in the regulation of neuronal survival and differentiation. Elevated levels of NGF are associated with the risk of post-traumatic stress disorder (PTSD). The trauma response leads to methylation of DNA nucleotides responsible for NGF expression. NGF levels have shown increased sympathetic fiber density proportional to NGF messenger RNA (mRNA) levels. NGF is also a seminal plasma protein commonly found in mammals. For example, NGF acts as an ovulation stimulating factor in camels and has been shown to have luteinizing effects in bulls. NGF has a potential function in the female reproductive system. For example, NGF plays an important role in ovulation induction, LH release, ovulation, luteal development, progesterone (P4) production, vascularization of luteal body, and gonadotropin response. Application of NGF to cattle enhances steroid production, luteal formation and function by increasing LH release, and leads to increased mRNA expression of markers of pregnancy and development downstream. In addition, the potential luteinizing effect of NGF could help overcome the current problem of early embryo loss. The similarity between human and bovine NGF protein sequence was 90.87%. Meanwhile, the similarity rate of human NGF with rat and mouse was 85.89% and 85.06%, respectively.
Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 1.463 ng/mL, corresponding to a specific activity is 6.835×105 units/mg.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.