Beryllon II
(Synonyms: 铍试剂 II) 目录号 : GC30700
BeryllonII是一种使用广泛的显色剂，可以用于检测Mo，Mg和Co等，同时可用于蛋白质的检测。
Cas No.:51550-25-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Beryllon II is a widely used chromogenic reagent that is used to determine many elements, such as Mo, Mg and Co, and also used for the determination of proteins.
Beryllon II is a widely used chromogenic reagent that has been used to determine many elements, such as Mo, Mg and Co. Beryllon II-Al3+ complex with the addition of protein can potently enhance the Rayleigh light scattering, and used to determine proteins[1].
[1]. Dong L, et al. Study of the reaction of proteins with Beryllon II-AlIII by the Rayleigh light scattering technique and its application. Analyst. 2001 May;126(5):707-11.
| Cas No. | 51550-25-5 | SDF | |
| 别名 | 铍试剂 II | ||
| Canonical SMILES | OC1=C(C(/N=N/C2=C(O)C(C(O)=CC(S([O-])(=O)=O)=C3)=C3C=C2S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C4)C4=CC(S(=O)([O-])=O)=C1.[Na+].[Na+].[Na+].[Na+] | ||
| 分子式 | C20H10N2Na4O15S4 | 分子量 | 738.52 |
| 溶解度 | DMSO : ≥ 64 mg/mL (86.66 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3541 mL | 6.7703 mL | 13.5406 mL |
| 5 mM | 0.2708 mL | 1.3541 mL | 2.7081 mL |
| 10 mM | 0.1354 mL | 0.677 mL | 1.3541 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A ratiometric fluorescence sensor for Be2+ based on Beryllon II/layered double hydroxide ultrathin films
A ratiometric fluorescence sensor for Be(2+) has been fabricated via alternate assembly of 2-(3,6-disulfo-8-hydroxynaphthylazo)-1,8-dihydroxynaphthalene-3,6-disulfonate (Beryllon II) and MgAl-LDH nanosheets on quartz substrates using the layer-by-layer (LBL) deposition technique. UV-vis absorption and the fluorescence emission spectroscopy indicate a stepwise and regular growth of the Beryllon II/LDH UTFs upon increasing deposition cycle. The film of Beryllon II/LDH possesses a periodic layered structure perpendicular to the substrate revealed by X-ray diffraction and scanning electron microscopy. Atomic force microscopy images show that the film surface is continuous and uniform. The Beryllon II/LDH UTFs display ratiometric fluorescence response for Be(2+) with a linear response range in 1.0×10(-7)-1.9×10(-6) mol L(-1) and a detection limit of 4.2×10(-9) mol L(-1). Furthermore, the ratiometric sensor exhibits good repeatability, high stability (thermal, storage and mechanical) as well as excellent selectivity toward Be(2+). XPS and Raman measurements demonstrate that the specific response of the sensor is attributed to the coordination between Be(2+) and Beryllon II in the UTF. The Beryllon II/LDH UTFs in this work can be potentially used as a chemosensor for the detection of Be(2+) in the environmental and biomedical field.
Electrochemical characterization of poly-beryllon II modified carbon paste electrode and its application to selective determination of pyrocatechol and hydroquinone
A poly-beryllon II film modified carbon paste electrode (PBL-II/CPE) has been constructed by electropolymerization method. Electrochemical characterization of the PBL-II/CPE was investigated by electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of pyrocatechol (PC) and hydroquinone (HQ) were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at the PBL-II/CPE in pH 7.0 phosphate buffer solution (PBS). The anodic peak currents were dramatically enhanced about threefold for PC at PBL-II/CPE. In addition, the modified electrode exhibits good selectivity toward selective determination of PC and HQ. By using DPV method, low detection limits of 0.8μM for PC and 1.0μM for HQ were obtained, respectively. Many outstanding advantages, such as wide linear ranges, low detection limits, excellent sensitivity, selectivity and stability confirmed that the PBL-II/CPE has promising analytical performance. Meanwhile, the electrode was successfully used for the determination of PC and HQ in local tap water sample and the results are satisfactory.
Study of the reaction of proteins with Beryllon II-AlIII by the Rayleigh light scattering technique and its application
The determination of proteins with tetrasodium 2-(3,6-disulfo-8-hydroxynaphthylazo)-1,8-dihydroxynaphthalene-3,6-disulfonate (Beryllon II) by Rayleigh light scattering (RLS) was studied. The weak RLS of the Beryllon II-bovine serum albumin (BSA) complex can be greatly enhanced by the addition of Al3+ in the pH range 5.6-7.2; there was a maximum RLS platform at 400-420 nm. Based on the reaction between Beryllon II, Al3+ and proteins, a new method for the determination of proteins was developed. This method is very sensitive [0.20-41.42 micrograms ml-1 for BSA and 0.18-48.15 micrograms ml-1 for human serum albumen (HSA)], rapid (< 2 min), simple (one step) and tolerant towards most interfering substances. The effects of different surfactants were also examined. Four samples of protein in human serum were determined; the maximum relative error was no more than 5% and the recovery was 96-105%.
Resin phase spectrophotometry of beryllium by an optical fiber and beryllon II
We have developed a resin phase spectrophotometric method to determine the amount of beryllium by detecting a change in absorbance of the resin phase. 8-Hydroxynaphthalene-3,6-disulphonic acid-(1-azo-2')-1',8'-dihydroxynaphthalene-3',6'-disulphonic acid tetrasodium salt (beryllon II) immobilized by a strong base anion-exchange resin on the end of a bifurcated optical fiber bundle was chosen as a material sensitive to beryllium. Experiments were made using a home-made optical fiber spectrophotometer. The properties of the resin phase proved to be good for continual use of 350 times during a month. The reaction order between immobilized beryllon II and beryllium in solution was determined by kinetic theory and experiments. This method was also used to determine the amount of beryllium in beryllium-copper alloy; the response was linear from 0.43 to 2.60 mug/ml at pH 12.5 and 676 nm. The effect of interfering ions on the determination of beryllium was studied at the same time.