9-Fluorenylmethyl carbazate
(Synonyms: 酰肼) 目录号 : GC652339-Fluorenylmethyl carbazate 作为荧光团试剂,可用于糖链的荧光检测。
Cas No.:35661-51-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
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- Datasheet
9-Fluorenylmethyl carbazate is used as a fluorophore reagent for a fluorimetric detection of glycans[1].
[1]. Kinoshita M, et al. A practical method for preparing fluorescent-labeled glycans with a 9-fluorenylmethyl derivative to simplify a fluorimetric HPLC-based analysis. J Pharm Biomed Anal. 2020 Jul 15;186:113267.
Cas No. | 35661-51-9 | SDF | Download SDF |
别名 | 酰肼 | ||
分子式 | C15H14N2O2 | 分子量 | 254.28 |
溶解度 | 储存条件 | Store at -20°C | |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.9327 mL | 19.6634 mL | 39.3267 mL |
5 mM | 0.7865 mL | 3.9327 mL | 7.8653 mL |
10 mM | 0.3933 mL | 1.9663 mL | 3.9327 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A practical method for preparing fluorescent-labeled glycans with a 9-fluorenylmethyl derivative to simplify a fluorimetric HPLC-based analysis
J Pharm Biomed Anal 2020 Jul 15;186:113267.PMID:32240925DOI:10.1016/j.jpba.2020.113267.
Analysis of glycans in glycoproteins is often performed by liquid chromatography (LC) separation coupled with fluorescence detection and/or mass spectrometric detection. Enzymatically or chemically released glycans from glycoproteins are usually labeled by reductive amination with a fluorophore reagent. Although labeling techniques based on reductive amination have been well-established as sample preparation methods for fluorometric HPLC-based glycan analysis, they often include time-consuming and tedious purification steps. Here, we reported an alternative fluorescent labeling method based on the synthesis of hydrazone and its reduction using 9-Fluorenylmethyl carbazate (Fmoc-hydrazine) as a fluorophore reagent. Using isomaltopentaose and N-glycans from human IgG, we optimized the Fmoc-labeling conditions and purification procedure of Fmoc-labeled N-glycans and applied the optimized method for the analysis of N-glycans released from four glycoproteins (bovine RNase B, human fibrinogen, human α1-acid glycoprotein, and bovine fetuin). The complete workflow for preparation of fluorescent-labeled N-glycans takes a total of 3.5 h and is simple to implement. The method presented here lowers the overall cost of a fluorescently labeled N-glycan and will be practically useful for the screening of disease-related glycans or routine analysis at an early stage of development of biopharmaceuticals.
A Shelf Stable Fmoc Hydrazine Resin for the Synthesis of Peptide Hydrazides
Pept Sci (Hoboken) 2022 Sep;114(5):e24268.PMID:36387422DOI:10.1002/pep2.24268.
C-terminal hydrazides are an important class of synthetic peptides with an ever expanding scope of applications, but their widespread application for chemical protein synthesis has been hampered due to the lack of stable resin linkers for synthesis of longer and more challenging peptide hydrazide fragments. We present a practical method for the regeneration, loading, and storage of trityl-chloride resins for the production of hydrazide containing peptides, leveraging 9-Fluorenylmethyl carbazate. We show that these resins are extremely stable under several common resin storage conditions. The application of these resins to solid phase peptide synthesis (SPPS) is demonstrated through the synthesis of the 40-mer GLP-1R agonist peptide "P5". These studies support the broad utility of Fmoc-NHNH-Trt resins for SPPS of C-terminal hydrazide peptides.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nuclear magnetic resonance analyses of end-functionalized saccharidic polymers: an example of a useful analytical technique combination
Rapid Commun Mass Spectrom 2004;18(6):664-72.PMID:15052577DOI:10.1002/rcm.1385.
The living cationic polymerization of a saccharidic monomer (1,2:3,4-di-O-isopropylidene-6-O-(2-vinyloxyethyl)-D-galactopyranose, GVE) gives rise to aldehyde end-capped polymers (PGVE--CHO) of low molar masses (<10 000 Da) and low molar mass distribution (<1.2). These polymers were derivatized by selective introduction of either hexamethylenediamine (PGVE-NH2) or 9-Fluorenylmethyl carbazate (PGVE-Fmoc) end groups. The resulting polymers were fully characterized by complementary use of nuclear magnetic resonance (1H NMR) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The expected structure according to the polymerization mechanism, the occurrence of side reactions and the success of the post-functionalization reactions were confirmed.