5-Hydroxytryptamine (Serotonin)
(Synonyms: 5-羟基色胺) 目录号 : GC18704
5-羟色胺是一种神经递质，是介导人体多种中枢和外周功能的关键信使。
Cas No.:50-67-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment [1]: |
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Cell lines |
Osteoblasts |
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Preparation method |
Osteoblasts at passage 3 were used to perform all cell studies and were divided into six groups, which were cultured in the presence of various concentrations of 5-HT : 0 M (control group) and 1 nM, 10 nM, 100 nM, 1 µM, and 10 µM (experimental groups). Primary osteoblasts were incubated with 5-HT for 2 days, and the proliferation rate was measured. |
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Reaction Conditions |
0 M and 1 nM, 10 nM, 100 nM, 1 µM, and 10 µM; 2 days |
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Applications |
Growth of osteoblasts treated with serotonin (1 nM-10 µM) was inhibited compared to controls. This influence was alleviated in the 10 µM and 1 µM groups (relatively high concentrations). |
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Animal experiment [2]: |
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Animal models |
C57BL/6 mice |
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Preparation method |
Twenty-five adult female C57BL/6 mice were divided into five groups. Colon tumours in mice were induced by AOM and DSS. Respectively, 10 mg/kg 5-HT was intraperitoneally injected (5-HT group), 20 mg/kg citalopram in normal saline was administered intragastrically (citalopram group), and then both 5-HT and citalopram were administered in combination (combined group). Following the procedure, the mice were sacrificed after 90 days, and their blood serum was collected to quantify 5-HT contents. The tumours were separated from each animal, and their sizes and volumes were measured. |
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Dosage form |
10 mg/kg, i.p. |
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Applications |
After 90 days of treatment with 10 mg/kg 5-HT, the tumour number and volume in the mice’s intestinal mucosa were 11.60 ± 1.52 and 14.35 ± 2.72 mm3, respectively. 5-HT promotes primary colon carcinogenesis in mice. |
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References: [1] Dai SQ, et al. Serotonin regulates osteoblast proliferation and function in vitro. Braz J Med Biol Res. 2014 Sep;47(9):759-65. [2] Yu H, et al. Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression. Cell Commun Signal. 2023 Apr 12;21(1):75. |
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5-hydroxytryptamine, (5-HT, Serotonin) a neurotransmitter, is a key messenger that mediates several central and peripheral functions in the human body[1]. 5-HT is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 µM[2].
In vitro, 5-Hydroxytryptamine (1 nmol/L-10 micromol/L) caused vasoconstriction in a concentration-dependent manner[3]. In vitro, 10 μM 5-HT proliferates colon cancer cells through enhancing YAP (Yes-associated protein) expression[1]. In vitro, for left lower lobe of lungs isolated from the dog, infusion of 5-hydroxytryptamine (100 micrograms/min) increased the pulmonary pre- and post-capillary resistances, but did not change the filtration coefficient[4].
In vivo, topical 5-HT (1-10 nmols) or the 5-HT1/7 receptor agonist, 5-carboxamidotryptamine (10-30 nM), dilated third- and fourth-order arterioles, responses that were abolished by 1 μM SB269970, a selective 5-HT7 receptor antagonist[5].
References:
[1] Yu H, et al. Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression. Cell Commun Signal. 2023 Apr 12;21(1):75.
[2] Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25.
[3] Tanaka N, et al. Both 5-hydroxytryptamine 5-HT2A and 5-HT1B receptors are involved in the vasoconstrictor response to 5-HT in the human isolated internal thoracic artery. Clin Exp Pharmacol Physiol. 2008 Jul;35(7):836-40.
[4] Sumita T, et al. 5-Hydroxytryptamine receptor subtypes participating in pulmonary edema in dogs. Eur J Pharmacol. 1989 May 2;164(1):69-75.
[5] Jackson WF, et al. 5-HT7 receptors mediate dilation of rat cremaster muscle arterioles in vivo.Microcirculation. 2023 Aug;30(5-6):e12808.
5-羟色胺是一种神经递质，是介导人体多种中枢和外周功能的关键信使[1]。
在体外，5-羟色胺（1 nmol/L-10 micromol/L）以浓度依赖的方式引起血管收缩[2]。在体外，10 μM 5-HT通过增强YAP（Yes-associated protein）的表达来增殖结肠癌细胞[1]。在体外，对于从狗身上分离的左肺下叶，输注5-羟色胺（100微克/分钟）会增加肺毛细血管前和毛细血管后的阻力，但不会改变过滤系数[3]。
在体内，局部5-HT（1-10 nmols）或5-HT1/7受体激动剂5-甲酰胺色氨酸（10-30 nM）扩张第三和第四级小动脉，反应被1μM SB269970（一种选择性5-HT7受体拮抗剂）消除[4]。
| Cas No. | 50-67-9 | SDF | |
| 别名 | 5-羟基色胺 | ||
| 分子式 | C10H12N2O | 分子量 | 176.22 |
| 溶解度 | DMSO: 100 mg/mL (567.47 mM) | 储存条件 | Store at 2-8°C , protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.6747 mL | 28.3736 mL | 56.7472 mL |
| 5 mM | 1.1349 mL | 5.6747 mL | 11.3494 mL |
| 10 mM | 0.5675 mL | 2.8374 mL | 5.6747 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
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