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5-FAM SE Sale

(Synonyms: 5-羧基荧光素琥珀酰亚胺酯) 目录号 : GC30174

A fluorescent cleavage product of CFDA-SE

5-FAM SE Chemical Structure

Cas No.:92557-80-7

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥727.00
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10mg
¥662.00
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50mg
¥1,980.00
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100mg
¥3,150.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

CFSE is a fluorescent product of CFDA-SE cleavage by intracellular esterases.1 CFSE lacks the diacetate groups of CFDA-SE and, as a result, is less cell permeable. CFSE fluorescence is cytoplasmic, and it has excitation/emission maxima of 491 and 518 nm, respectively.2 It covalently couples to intracellular molecules via its succinimidyl group and can be retained within cells for at least eight weeks. The dilution of CFSE fluorescence resulting from cell division can be used to analyze cell proliferation.3 Its fluorescence can also be used to track cell migration in vivo.4

1.Breeuwer, P., Drocourt, J., Rombouts, F.M., et al.A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl esterAppl. Environ. Microbiol.62(1)178-183(1996) 2.Weston, S.A., and Parish, C.R.New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopyJ. Immunol. Methods133(1)87-97(1990) 3.Lyons, A.B.Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilutionJ. Immunol. Methods243(1-2)147-154(2000) 4.Parish, C.R., Glidden, M.H., Quah, B.J.C., et al.Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferationCurr. Protoc. Immunol.Supp. 844.9.1-4.9.13(2009)

Chemical Properties

Cas No. 92557-80-7 SDF
别名 5-羧基荧光素琥珀酰亚胺酯
Canonical SMILES O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)ON6C(CCC6=O)=O
分子式 C25H15NO9 分子量 473.39
溶解度 DMSO : 25 mg/mL (52.81 mM) 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL
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Research Update

Novel Au-Se Nanoprobes for Specific Thrombin Detection in Diagnosis of Lung Cancer

Thrombin is associated with malignant tumors and promotes tumor development, metastasis, and angiogenesis, therefore its identification especially in lung cancer cells is crucial. Because the interference of in vivo biothiols caused false positive findings with prior gold fluorescent nanoprobes, in this manuscript, an Au-selenol(Se) nanoprobe (5-FAM-peptide-Se-AuNPs) that could specifically detect thrombin was designed and compared to traditional Au-S nanoprobes. For reaching this goal, fluorophore-bearing thrombin-specific peptide containing selenol at the end was synthesized. The nanoprobe may be broken by thrombin to regain its fluorescence in lung cancer cells, allowing for high-sensitivity thrombin detection. Since the Au-Se bond is more stable than the Au-S bond, the accuracy of the detection results can be guaranteed. The probe synthesis method is simple and cost-effective, as well as having high biocompatibility. Low concentrations of thrombin can be detected and imaged in lung cancer cells. The synthetic method of this probe opens up new avenues for the application of Au-Se bonds.

The association between lumican gene polymorphisms and high myopia

Purposes: Lumican (LUM) is one of the major extracellular matrix components of the sclera. Increasing evidence suggests that changes in the structure and composition of the sclera are major factors in regulating scleral integrity and axial elongation of the eye, as in myopia.
Patients and methods: Patients (n=182; age range, 17-24 years) were with a myopic spherical equivalent (SE)>6.5 diopters (D) and the control group comprised individuals (n=78; age range, 17-25 years) were with a myopic SE<0.5 D. The DNA fragments were separated by horizontal electrophoresis on 3% agarose gels. The forward primer was labelled with a 5' FAM and the reaction products were detected using a 3100 Genetic Analyzer.
Results: The polymorphisms detected in this study were LUMc.601, LUM-59, LUM-628, and LUM-1554. Moreover, the haplotype distributions of Ht1 (C/A/CC/T), Ht2 (C/A/--/T), Ht3 (T/A/CC/C), Ht4 (T/--/CC/T), Ht5 (T/--/CC/C), and Ht6 (T/--/--/C) of these polymorphisms were compared between the two groups. The haplotype frequencies of Ht1, Ht2, Ht5, and Ht6 differed significantly between the two groups (P=2.08x10(-5), odds ratio (OR): 2.19, 95% confidence interval (CI): 1.52-3.15; P=2.2x10(-5), OR: 0.39, 95% CI: 0.25-0.61; P=2.7x10(-5), OR: 0.36, 95% CI: 0.22-0.59; P=3.7x10(-5), OR: 4.71, 95% CI: 2.12-10.5, respectively).
Conclusions: These observations suggest that the four polymorphisms of the LUM promoter contribute to the pathogenesis of high myopia. Understanding the functions of LUM in myopia helps us design new methods in treating and preventing myopia.

Inhibitor screening and selectivity assessment against multiple cellular protein kinases by capillary electrophoresis with laser-induced fluorescence detection

A method that can be used for screening protein kinase inhibitors (PKIs) and simultaneously assessing their selectivity is described. The method is based on simultaneously assaying multiple cellular protein kinases by performing capillary electrophoresis (CE) separation and measuring the peak areas of the phosphorylated substrate peptides. The powerful separation capability of CE combined with the highly sensitive and selective laser-induced fluorescence (LIF) detector enables the direct screening of PKIs against cell lysates, which are used as an inexpensive source of enzymes. Four cell lines, three specific substrate peptides labeled with 5-carboxyfluorescein (5-FAM), two relative specific PKIs (TBB and H-89) and one non-specific PKI (staurosporine) were utilized to prove the methodology. With this method, the inhibitory activity of the tested compounds against multiple protein kinases was identified in parallel by comparing the peak areas of the phosphorylated substrates with those obtained in the absence of any inhibitors. The reduced peak area of the phosphorylated substrate definitively represents a positive screening result. Simultaneously, assaying the inhibition of one inhibitor against mutiple cellular protein kinases enables the assessment of its selectivity. Compared to the conventional, single-target screening format, the cell lysate-based multi-target method is more informative, more straightforward and more cost effective.