5-Benzyloxygramine
(Synonyms: 5-苄氧基芦竹碱) 目录号 : GC628085-Benzyloxygramine 是 N 蛋白、蛋白互作的原位稳定剂,具有抗病毒和稳定 N-NTD 蛋白的功能。
Cas No.:1453-97-0
Sample solution is provided at 25 µL, 10mM.
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5-Benzyloxygramine is a N protein PPI orthosteric stabilizer that exhibits both antiviral and N-NTD protein-stabilizing activities[1].
5-benzyloxygramine stabilizes the N-NTD dimers through simultaneous hydrophobic interactions with both partners, resulting in abnormal N protein oligomerization that was further confirmed in the cell[1].
[1]. Shan-Meng Lin, et al. Structure-Based Stabilization of Non-native Protein-Protein Interactions of Coronavirus Nucleocapsid Proteins in Antiviral Drug Design. J Med Chem. 2020 Mar 26;63(6):3131-3141.
Cas No. | 1453-97-0 | SDF | |
别名 | 5-苄氧基芦竹碱 | ||
分子式 | C18H20N2O | 分子量 | 280.36 |
溶解度 | DMSO : 250 mg/mL (891.71 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.5668 mL | 17.8342 mL | 35.6684 mL |
5 mM | 0.7134 mL | 3.5668 mL | 7.1337 mL |
10 mM | 0.3567 mL | 1.7834 mL | 3.5668 mL |
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Actions of some analogues of 5-hydroxytryptamine on the isolated rat uterus and the rat fundus strip preparations
Br J Pharmacol Chemother 1959 Jun;14(2):265-72.PMID:13662587DOI:10.1111/j.1476-5381.1959.tb01397.x.
The search for substances which antagonize 5-hydroxytryptamine, and for those which act like it, has been extended to cover 5-substituted indoles which are analogues of 5-hydroxytryptamine, rather than analogues of tryptamine like the compounds previously studied by us. The isolated rat uterus and rat fundus strip preparations have been used to determine activity. The relationships between structure and activity of the compounds studied were not the same on the two preparations nor were they the same from one homologous series to another. These differences may be partly explained by the presence of amine oxidase in the rat fundus, as Vane (1959) has suggested, and by supposing that it is absent from the rat uterus. None of the compounds had marked antagonist activity. The most active antagonist on the rat uterus was 3-(2-aminopropyl)-5-benzyloxyindole, but this was less potent than 5-Benzyloxygramine. On the rat fundus strip, however, the only antagonist, 5-benzyloxy-3-(2-dimethylaminoethyl)indole, was more active than 5-Benzyloxygramine. On both preparations the most active stimulant was 3-(2-aminopropyl)-5-benzyloxyindole, which was about half as potent as 5-hydroxytryptamine. The next most active were 3-(2-aminopropyl)-5-methoxyindole and bufotenine.
Structure-Based Stabilization of Non-native Protein-Protein Interactions of Coronavirus Nucleocapsid Proteins in Antiviral Drug Design
J Med Chem 2020 Mar 26;63(6):3131-3141.PMID:32105468DOI:10.1021/acs.jmedchem.9b01913.
Structure-based stabilization of protein-protein interactions (PPIs) is a promising strategy for drug discovery. However, this approach has mainly focused on the stabilization of native PPIs, and non-native PPIs have received little consideration. Here, we identified a non-native interaction interface on the three-dimensional dimeric structure of the N-terminal domain of the MERS-CoV nucleocapsid protein (MERS-CoV N-NTD). The interface formed a conserved hydrophobic cavity suitable for targeted drug screening. By considering the hydrophobic complementarity during the virtual screening step, we identified 5-Benzyloxygramine as a new N protein PPI orthosteric stabilizer that exhibits both antiviral and N-NTD protein-stabilizing activities. X-ray crystallography and small-angle X-ray scattering showed that 5-Benzyloxygramine stabilizes the N-NTD dimers through simultaneous hydrophobic interactions with both partners, resulting in abnormal N protein oligomerization that was further confirmed in the cell. This unique approach based on the identification and stabilization of non-native PPIs of N protein could be applied toward drug discovery against CoV diseases.
Two kinds of tryptamine receptor
Br J Pharmacol Chemother 1957 Sep;12(3):323-8.PMID:13460238DOI:10.1111/j.1476-5381.1957.tb00142.x.
There are two kinds of tryptamine receptor in the guinea-pig ileum, namely the M receptors which can be blocked with morphine and the D receptors which can be blocked with dibenzyline. Atropine, an atropine-like drug, cocaine, and methadone inhibit effects due to the M receptors, even after dibenzyline, but have no additional effect after morphine. Lysergic acid diethylamide, dihydroergotamine and 5-Benzyloxygramine inhibit effects due to the D receptors, even after morphine, but have no additional effect after dibenzyline. The M receptors are probably in the nervous tissue and the D receptors are probably in the muscles.
Actions of some analogues of tryptamine on the isolated rat uterus and on the isolated rat fundus strip preparations
Br J Pharmacol Chemother 1959 Mar;14(1):99-107.PMID:13651585DOI:10.1111/j.1476-5381.1959.tb00934.x.
Some 3-(2-dialkylaminoethyl)-, 3-(2-alkylaminoethyl)-, and 3-(2-dialkylaminoethyl)-2-methylindoles have been synthesized and tested, along with 5-Benzyloxygramine, 5-benzyloxy-3-(2-dimethylaminoethyl)-, 3-(2-aminopropyl)- and 3-(2-aminobutyl)-indoles, on the rat uterus and rat fundus strip. 5-Benzyloxy-3-(2-dimethylaminoethyl)indole, even though it contains an ethylene side-chain, was a less potent antagonist of 5-hydroxytryptamine than was 5-Benzyloxygramine. The remaining compounds were still less active. There are differences between the ability of some of them to antagonize 5-hydroxytryptamine and to antagonize tryptamine. 3-(2-Dimethylaminoethyl)-2-methylindole, in particular, shows a considerable degree of specific antagonism of 5-hydroxytryptamine on both tissues.Nearly all the compounds stimulate the preparations, some combining antagonism of 5-hydroxytryptamine at low concentrations with stimulant activity at higher concentrations. The most active compound is 3-(2-dipropylaminoethyl)indole. On the rat fundus strip this has, on a molar basis, 1/40th of the stimulant activity of 5-hydroxytryptamine, and is 20 times as active as tryptamine: on the rat uterus it is only 1/200th as active as 5-hydroxytryptamine, and equal in activity to tryptamine.
Two kinds of tryptamine receptor. 1957
Br J Pharmacol 1997 Feb;120(4 Suppl):134-9; discussion 132-3.PMID:9142401DOI:10.1111/j.1476-5381.1997.tb06789.x.
There are two kinds of tryptamine receptor in the guinea-pig ileum, namely the M receptors which can be blocked with morphine and the D receptors which can be blocked with dibenzyline. Atropine, an atropine-like drug, cocaine, and methadone inhibit effects due to the M receptors, even after dibenzyline, but have no additional effect after morphine. Lysergic acid diethylamide, dihydroergotamine and 5-Benzyloxygramine inhibit effects due to the D receptors, even after morphine, but have no additional effect after dibenzyline. The M receptors are probably in the nervous tissue and the D receptors are probably in the muscles.