4-Chloro-1-naphthol
目录号 : GC67213
4-Chloronaphthalen-1-ol 是辣根过氧化物酶的底物。4-Chloronaphthalen-1-ol 可用于在蛋白质印迹中显示蛋白质条带。
Cas No.:604-44-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
4-Chloronaphthalen-1-ol is a substrate of horseradish peroxidase. 4-Chloronaphthalen-1-ol can be used to visualize protein bands in western blotting[1][2].
[1]. Elias JM, et, al. A rapid, sensitive myeloperoxidase stain using 4-chloro-1-naphthol. Am J Clin Pathol. 1980 Jun;73(6):797-9.
[2]. Hou L, et, al. Graphene oxide-labeled sandwich-type impedimetric immunoassay with sensitive enhancement based on enzymatic 4-chloro-1-naphthol oxidation. Biosens Bioelectron. 2013 Sep 15;47:149-56.
| Cas No. | 604-44-4 | SDF | Download SDF |
| 分子式 | C10H7ClO | 分子量 | 178.62 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.5985 mL | 27.9924 mL | 55.9848 mL |
| 5 mM | 1.1197 mL | 5.5985 mL | 11.197 mL |
| 10 mM | 0.5598 mL | 2.7992 mL | 5.5985 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
4-Chloro-1-naphthol
Acta Crystallogr C 2009 May;65(Pt 5):o207-10.PMID:19407417DOI:10.1107/S0108270109010063.
Molecules of the title compound, C(10)H(7)ClO, (I), are connected by a single strong O-H...O hydrogen bond into a simple C(2) chain, which runs parallel to the c axis and is additionally stabilized by intermolecular pi-pi stacking interactions. The significance of this study lies in the comparison drawn between the crystal structure of (I) and those of several of its simple analogues. This comparison shows a close similarity in the packing of the molecules that form pi-stacks along the shortest crystallographic axes. A substantial spatial overlap is observed between adjacent molecules in such a pi-stack, depending mainly on the kind of substituent.
A rapid, sensitive myeloperoxidase stain using 4-Chloro-1-naphthol
Am J Clin Pathol 1980 Jun;73(6):797-9.PMID:6156595DOI:10.1093/ajcp/73.6.797.
The substitution of 4-Chloro-1-naphthol for benzidine-containing compounds used in the myeloperoxidase stain for distinguishing granulocytes from lymphoid blood elements is described. 4-Chloro-1-naphthol is an acceptable substitute for benzidine compounds, which are considered to be carcinogenic. The feasibility of using this method on peripheral blood and bone-marrow films is discussed.
Visualization of antigen on nitrocellulose membrane by the oxidative coupling reaction of N,N'-dimethyl-p-phenylenediamine and 4-Chloro-1-naphthol
Anal Biochem 1989 Nov 15;183(1):9-12.PMID:2482680DOI:10.1016/0003-2697(89)90162-0.
A method which allows the highly sensitive and simple immunodetection of antigen-antibody complexes on nitrocellulose papers has been developed. The method is a modification of the procedure known as the "Nadi reaction" (oxidative coupling of 1-naphthol and N,N'-dimethyl-p-phenylendiamine) for histochemical purposes. Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membranes. Bound proteins were first reacted with a primary antibody and then with a horseradish peroxidase-labeled second antibody. The antigen-antibody complexes on the membranes were visualized with the oxidative coupling solution containing N,N'-dimethyl-p-phenylendiamine and 4-Chloro-1-naphthol. Sensitivity was enhanced 4 to 16 times by the new method relative to that of the 4-Chloro-1-naphthol method or the 3,3'-diaminobenzidine method.
Graphene oxide-labeled sandwich-type impedimetric immunoassay with sensitive enhancement based on enzymatic 4-Chloro-1-naphthol oxidation
Biosens Bioelectron 2013 Sep 15;47:149-56.PMID:23567634DOI:10.1016/j.bios.2013.02.035.
A new sandwich-type impedimetric immunosensor based on functionalized graphene oxide nanosheets with a high ratio of horseradish peroxidase (HRP) and detection antibody was developed for the detection of carcinoembryonic antigen (CEA) by coupling with enzymatic biocatalytic precipitation of 4-Chloro-1-naphthol (4-CN) on the captured antibody-modified glassy carbon electrode. Two molecular tags (with and without the graphene oxide nanosheets) were investigated for the detection of CEA and improved analytical features were acquired with the graphene-based labeling. With the labeling method, the performance and factors influencing the properties of the impedimetric immunosensors were also studied and evaluated. Under the optimal conditions, the dynamic concentration range of the impedimetric immunosensors spanned from 1.0pgmL(-1) to 80ngmL(-1) CEA with a detection limit (LOD) of 0.64pgmL(-1). Intra- and inter-assay coefficients of variation were less than 7.5% and 11%, respectively. Additionally, the methodology was evaluated for CEA analysis of 10 clinical serum samples and 5 diluted serum samples, receiving in a good accordance with the results obtained by the impedimetric immunoassay and the commercialized electrochemiluminescent method.
5-Amino-1-naphthol: two-dimensional sheets built up from R(4)(4)(18) rings formed by O-H...N, N-H...O and pi-pi interactions
Acta Crystallogr C 2009 Nov;65(Pt 11):o565-8.PMID:19893237DOI:10.1107/S0108270109038694.
The crystal structure of the title compound, C(10)H(9)NO, (I), contains intermolecular O-H...N and N-H...O hydrogen bonds which together form sheets parallel to the (001) plane containing rings with an unusual R(4)(4)(18) motif. These rings are additionally stabilized by an intermolecular pi-pi stacking interaction. The significance of this study lies in the comparison drawn between the molecular structure of (I) and those of related compounds (1,5-diaminonaphthalene, 8-amino-2-naphthol, 3-amino-2-naphthol and aniline), which shows a close similarity in the noncoplanar orientation of the amine group and the aromatic moiety. Comparison of the crystal structures of (I) and several of its simple analogues (1-naphthol, naphthalene-1,4-diol, naphthalene-1,5-diol and 4-Chloro-1-naphthol) shows a close similarity in the packing of the molecules, which form pi-stacks along the shortest crystallographic axes with a substantial spatial overlap between adjacent molecules.