28-Homobrassinolide
(Synonyms: 28-高芸苔素内脂) 目录号 : GC6771328-Homobrassinolide 是一种植物类固醇。28-Homobrassinolide 可用于胆固醇和葡萄糖体内平衡的研究。
Cas No.:82373-95-3
Sample solution is provided at 25 µL, 10mM.
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28-Homobrassinolide is a phytosteroid. 28-Homobrassinolide can be used for the research of cholesterol and glucose homeostasis[1].
28-Homobrassinolide (5-20 μM) 表明 HepG2 细胞中的葡萄糖利用以及 ABCA1 和 SREBP2 蛋白表达增加了 2 倍[1]。
28-Homobrassinolide 降低组织葡萄糖和胆固醇水平,增加胆固醇水平和组织己糖激酶活性[1]。
Cell Cytotoxicity Assay[1]
Cell Line: | HepG2 cells |
Concentration: | 1 µM, 5 µM, 10 µM, 20 µM, 30 µM, 40 µM, and 80 µM |
Incubation Time: | 24 h |
Result: | The IC50 value of 40.57µM obtained by the MTT assay. |
Western Blot Analysis[1]
Cell Line: | HepG2 cells |
Concentration: | 5, 10, and 20 μM |
Incubation Time: | 12 h |
Result: | Exhibited an increase in both ABCA1 and SREBP2 markers. |
28-HB(1-20 μg/天;持续 6 周)显示高脂肪饮食喂养的 C57BL/6 小鼠的主动脉脂肪沉积和血清标志物水平显着降低[1]。
Animal Model: | High-fat diet-fed C57BL/6 mice[1] |
Dosage: | 1-20 μg/day |
Administration: | For 6 weeks |
Result: | Observed a significant decrease in lipid deposition. |
[1]. Victor Mukherjee, et al. Phytosteroid 28-homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation. Steroids. 2021 Jan;165:108756.
Cas No. | 82373-95-3 | SDF | Download SDF |
别名 | 28-高芸苔素内脂 | ||
分子式 | C29H50O6 | 分子量 | 494.7 |
溶解度 | DMSO : 20 mg/mL (40.43 mM; Need ultrasonic) | 储存条件 | Store at 2-8°C,protect from light |
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1 mM | 2.0214 mL | 10.1071 mL | 20.2143 mL |
5 mM | 0.4043 mL | 2.0214 mL | 4.0429 mL |
10 mM | 0.2021 mL | 1.0107 mL | 2.0214 mL |
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Phytosteroid 28-Homobrassinolide targets cholesterol and glucose homeostasis implicating ABCA1 and SREBP role in regulation
Steroids 2021 Jan;165:108756.PMID:33171131DOI:10.1016/j.steroids.2020.108756.
Oxysterols are natural ligands of certain nuclear receptors known as liver X receptors (LXR). LXRs are regulators of fatty acid, cholesterol, and glucose homeostasis. Dietary phyto-oxysterol 28-Homobrassinolide (28-HB) has been demonstrated to transactivate rat LXR α and β. In this study we assessed the potential of 28-HB to effect such changes in - (1) human HepG2 cancer cell line, (2) isolated perfused goat liver, and (3) high-fat diet-fed C57BL/6J mice. Serum and perfusate marker levels along with hexokinase activity were determined through enzyme assays. Fat deposition was studied by Oil Red O staining, ATP-binding cassette transporter (ABCA1), and sterol regulatory element-binding transcription factor 2 (SREBP2) protein expression by Western blot and their mRNA expression through real-time PCR. In HepG2 cells, 28-HB (5-20 μM) treatment indicated a 2-fold increase in glucose utilization and ABCA1 and SREBP2 protein expression within 12 h. Tissue glucose and cholesterol levels decreased in 28-HB perfused goat liver within 2 h, whereas cholesterol level increased 54% in the perfusate (p < 0.05) and tissue hexokinase activity increased 23% (p < 0.05). Glucokinase, ABCA1, and SREBF1 gene expression increased 2.6, 5.37, and 2.85 fold respectively in the perfused tissue after 4 h. High-fat diet-fed C57BL/6J mice when treated with 28-HB (1-20 µg/day) for 6 weeks exhibited a marked decrease in aortic fat deposit and serum marker levels. Our study suggests that 28-HB modulates cholesterol and glucose homeostasis in animal cells through activation of LXR involving ABCA1 and SREBP-1 and 2 augmentations.
Therapeutic effect of 28-Homobrassinolide on leukotriene synthesis in leukemia cells
Cytotechnology 2018 Feb;70(1):431-438.PMID:29101583DOI:10.1007/s10616-017-0158-4.
The present study was aimed to investigate the effect of 28-Homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C4 (LTC4) and Leukotriene B4 (LTB4) were determined in the cell and cell lysates. Intracellular Ca2+ was determined in the intact cells. Phospholipases A2 (PLA2) expression was determined in the cells using immunofluorescence. LTC4 and LTB4 were tremendously increased in IgE-loaded 2H3 cells. However, these levels were significantly reduced following treatment with 28-Homobrassinolide. The intracellular Ca2+ level was not altered by treatment. Expression of PLA2 was significantly reduced following treatment with 28-Homobrassinolide. Taking all these data together, it is suggested that the 28-Homobrassinolide may be a potential therapeutic agent to inhibit leukotriene synthesis in 2H3 cells.
28-Homobrassinolide: a novel oxysterol transactivating LXR gene expression
Mol Biol Rep 2014 Nov;41(11):7447-61.PMID:25091941DOI:10.1007/s11033-014-3632-5.
Cholesterol is the template for steroid hormone biosynthesis. Cholesterol homeostasis is regulated by Cyt-P450 oxygenated cholesterols acting as ligands on LXR-α and LXR-β transcription factors that are now emerging as drug targets. Heterodimerization of LXRs with retinoic acid receptor is considered a prerequisite for target gene activation. Dietary plant oxysterol 28-Homobrassinolide (28-HB) is a proven antihyperglycemic and a pro-steroidogenic agent in the rat. Whether 28-HB has a role in LXR gene expression was therefore investigated using oral gavage (15 days) of 28-HB (333 µg/kg b w) to normal and diabetic rat. PCR amplified LXR-α and β mRNA transcripts from treated rat liver and testis exhibited quantitative differences in their expression. Conformational differences in 28-HB docking to LXR-α and β binding domains were also noted through in silico studies, LXR-β adopting lesser specificity. We report that 28-HB transactivates LXR genes in the rat tissues.
28-Homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea
Sci Rep 2018 Jun 7;8(1):8735.PMID:29880861DOI:10.1038/s41598-018-27032-w.
Brassinosteroids (BRs) are a group of naturally occurring plant steroid hormones that can induce plant tolerance to various plant stresses by regulating ROS production in cells, but the underlying mechanisms of this scavenging activity by BRs are not well understood. This study investigated the effects of 28-Homobrassinolide (28-HBL) seed priming on Brassica juncea seedlings subjected to the combined stress of extreme temperatures (low, 4 °C or high, 44 °C) and salinity (180 mM), either alone or supplemented with 28-HBL treatments (0, 10-6, 10-9, 10-12 M). The combined temperature and salt stress treatments significantly reduced shoot and root lengths, but these improved when supplemented with 28-HBL although the response was dose-dependent. The combined stress alone significantly increased H2O2 content, but was inhibited when supplemented with 28-HBL. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) increased in response to 28-HBL. Overall, the 28-HBL seed priming treatment improved the plant's potential to combat the toxic effects imposed by the combined temperature and salt stress by tightly regulating the accumulation of ROS, which was reflected in the improved redox state of antioxidants.
28-Homobrassinolide mitigates boron induced toxicity through enhanced antioxidant system in Vigna radiata plants
Chemosphere 2011 Nov;85(10):1574-84.PMID:21899875DOI:10.1016/j.chemosphere.2011.08.004.
The objective of this study was to establish relationship between boron induced oxidative stress and antioxidant system in Vigna radiata plants and also to investigate whether brassinosteroids will enhance the level of antioxidant system that could confer tolerance to the plants from the boron induced oxidative stress. The mung bean (V. radiata cv. T-44) plants were administered with 0.50, 1.0 and 2.0 mM boron at 6 d stage for 7 d along with nutrient solution. At 13 d stage, the seedlings were sprayed with deionized water (control) or 10(-8) M of 28-Homobrassinolide and plants were harvested at 21 d stage to assess growth, leaf gas-exchange traits and biochemical parameters. The boron treatments diminished growth, water relations and photosynthetic attributes along with nitrate reductase and carbonic anhydrase activity in the concentration dependent manner whereas, it enhanced lipid peroxidation, electrolyte leakage, accumulation of H(2)O(2) as well as proline, and various antioxidant enzymes in the leaves of mung bean which were more pronounced at higher concentrations of boron. However, the follow-up application of 28-Homobrassinolide to the boron stressed plants improved growth, water relations and photosynthesis and further enhanced the various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and content of proline. The elevated level of antioxidant enzymes as well as proline could have conferred tolerance to the B-stressed plants resulting in improved growth, water relations and photosynthetic attributes.