10-Methyldodec-2-en-4-olide
目录号 : GC46402
A bacterial volatile lactone
Cas No.:874359-15-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >70.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
10-Methyldodec-2-en-4-olide is a volatile lactone that has been found in Streptomyces.1,2 It is active against the plant pathogenic fungus B. cinerea, the yeast S. cerevisiae, and the bacteria S. aureus, M. luteus, and M. phlei in an agar diffusion inhibition assay when used at a concentration of 140 μg per disc.3 10-Methyldodec-2-en-4-olide also inhibits induction of a GFP-based reporter by the quorum sensing molecule N-dodecanoylhomoserine lactone in an N-acylhomoserine lactone (AHL) P. putida biosensor strain.
1.Dickschat, J.S., Martens, T., Brinkhoff, T., et al.Volatiles released by a Streptomyces species isolated from the North SeaChem. Biodivers.2(7)837-865(2005) 2.Citron, C.A., Barra, L., Wink, J., et al.Volatiles from nineteen recently genome sequenced actinomycetesOrg. Biomol. Chem.13(9)2673-2683(2015) 3.Schulz, S., Dickschat, J.S., Kunze, B., et al.Biological activity of volatiles from marine and terrestrial bacteriaMar. Drugs8(12)2976-2987(2010)
| Cas No. | 874359-15-6 | SDF | |
| Canonical SMILES | CCC(C)CCCCCC(O1)C=CC1=O | ||
| 分子式 | C13H22O2 | 分子量 | 210.3 |
| 溶解度 | DMSO: soluble,Ethanol: soluble,Methanol: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7551 mL | 23.7756 mL | 47.5511 mL |
| 5 mM | 0.951 mL | 4.7551 mL | 9.5102 mL |
| 10 mM | 0.4755 mL | 2.3776 mL | 4.7551 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Volatiles released by a Streptomyces species isolated from the North Sea
Chem Biodivers 2005 Jul;2(7):837-65.PMID:17193176DOI:10.1002/cbdv.200590062
The North Sea Streptomyces strain GWS-BW-H5 was investigated by analyzing headspace extracts of agar-plate cultures (HE) or liquid cultures (LCE), obtained with a closed-loop stripping apparatus (CLSA), by GC/MS (Table 1). The volatile profile of the HE is dominated by the known volatiles (-)-geosmin (4) and 2-methyisoborneol (1). Small amounts of sesquiterpenes occur, which are present in a more-diverse structural variety and in higher quantities in the LCE. The different structures can be rationalized by few cationic intermediates along their biosynthetic pathway. The most-prominent difference between the two culture methods were the presence of the Me-branched gamma- and delta-lactones 31-38, not previously reported from nature, in the LCE. Major components were 10-methyldodecan-5-olide (34), 10-Methyldodec-2-en-4-olide (36), and 10-methyldodec-3-en-4-olide (38). The structures of all new lactones were verified by synthesis. Furthermore, more volatiles in higher amounts were produced by the liquid culture than by to the agar plate culture. Since 36 showed inhibitory growth effects against strain GWS-BW-H5, growth inhibition against twelve other strains isolated from the same habitat was tested. Antagonistic activity against four of the strains was observed, with a slightly higher threshold level than found for penicillin G, which was used in control experiments (Table 2).