Quinclorac
(Synonyms: 二氯喹啉酸) 目录号 : GC61230
Quinclorac是一种广泛应用于农业的除草剂，由于自由基的产生和抗氧化防御系统的变化而引起氧化应激。
Cas No.:84087-01-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Quinclorac, an herbicide widely applied in agriculture, induces oxidative stress due to free radical generation and changes in the antioxidant defense system[1].
[1]. Cavalheiro de Menezes C, et al. The effects of diphenyl diselenide on oxidative stress biomarkers in Cyprinus carpio exposed to herbicide quinclorac (Facet®). Ecotoxicol Environ Saf. 2012;81:91-97.
| Cas No. | 84087-01-4 | SDF | |
| 别名 | 二氯喹啉酸 | ||
| Canonical SMILES | O=C(C1=C2N=CC(Cl)=CC2=CC=C1Cl)O | ||
| 分子式 | C10H5Cl2NO2 | 分子量 | 242.06 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.1312 mL | 20.656 mL | 41.3121 mL |
| 5 mM | 0.8262 mL | 4.1312 mL | 8.2624 mL |
| 10 mM | 0.4131 mL | 2.0656 mL | 4.1312 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Comparison of quintrione and Quinclorac on mechanism of action
Pestic Biochem Physiol 2022 Feb;181:105007.PMID:35082030DOI:10.1016/j.pestbp.2021.105007.
Quintrione is a new post-emergence herbicide developed for use in rice; however, the mechanism of action remains unclear. We determined the phytotoxicity of quintrione, and the contributions of hormone levels and lipid peroxidation to phytotoxicity, by comparing them to those induced by Quinclorac. We also investigated 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and carotenoid content following treatment with quintrione by comparing them to those induced by Quinclorac and mesotrione. We found that quintrione and Quinclorac both inhibited the growth of Echinochloa crusgalli var. zelayensis, but that Quinclorac was a little more effective. At 24 h, quintrione and Quinclorac significantly increased ethylene production and the contents of abscisic acid (ABA) and indole acetic acid (IAA) compared with the control. No significant differences were observed between quintrione and Quinclorac on the three plant hormones. Quintrione and Quinclorac also induced the formation of malondialdehyde (MDA), which is associated with lipid peroxidation, with no significant difference between them. Carotenoid content was reduced in E. crusgalli var. zelayensis following treatments with quintrione, Quinclorac, and mesotrione. At 120 h, carotenoid contents were significantly higher following the quintrione and Quinclorac treatments, in comparison with mesotrione treatment. There were no significant differences between quintrione and Quinclorac in the inhibition of HPPD activity, and the effects of both were significantly less than the effect of mesotrione. In summary, E. crusgalli var. zelayensis was susceptible to both quintrione and Quinclorac. The mechanism of action of quintrione, like that of Quinclorac, was related to levels of plant hormones and lipid peroxidation; however, quintrione was a poor inhibitor of HPPD activity compared to mesotrione.
[Determination of Quinclorac in Livestock Products by LC-MS/MS]
Shokuhin Eiseigaku Zasshi 2022;63(5):177-181.PMID:36328473DOI:10.3358/shokueishi.63.177.
A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method was developed for determining Quinclorac in livestock products. Quinclorac was extracted from the samples using a solution of acetone and hydrochloric acid mixed in a 99 : 1 ratio. The crude extract was purified with ethyl acetate under basic conditions, followed by Quinclorac extraction with ethyl acetate under acidic conditions and analysis using LC-MS/MS. The average recoveries of Quinclorac from five livestock products (n=5) fortified at the maximum residue limits or 0.01 mg/kg ranged from 85.6 to 93.5%, with the precision of repeatability ranging from 1.7 to 6.8%. The quantification limit in this analytical method was 0.01 mg/kg. These results suggest that the developed method is useful for analyzing Quinclorac in livestock products.
Bioremediation of Quinclorac injury on tobacco by a rhizosphere bacterium
World J Microbiol Biotechnol 2022 Jul 1;38(9):147.PMID:35773599DOI:10.1007/s11274-022-03329-x.
The presence of herbicides residues in soil represents a serious problem for agriculture. Quinclorac is a common herbicide applied in rice field, but its residue can cause abnormal growth in successive crop of tobacco in Southern China. Remediation by microorganisms is considered to be an environmentally friendly method to remove such pollutants injury. The aims of this study were to obtain Quinclorac remediation isolates and to investigate the possible mechanism(s) of remediation. Six bacterial isolates were obtained from rhizosphere of rice-tobacco rotation fields, and were found to be capable of degrading Quinclorac on a mineral salt medium (MSM), with degradation efficiency ranging from 2.1 to 23.7%. Among these isolates, J5 had the highest degradation efficiency, and was identified as Klebsiella variicola based on phylogenetic analyses and a metabolic profile generating by Biolog GEN III system. Bioremediation of Quinclorac injury was confirmed using pot assays with tobacco, in which J5 reversed the detrimental effect of Quinclorac on leaf area, leaf number, and plant height. The J5 isolate also seemed to promote plant growth, in terms of tobacco seedling growth and seed germination, which were 2.2 times and 1.6 times higher compared to untreated control, respectively. The mechanisms of plant growth promoting (PGP) traits were found to involve nitrogen-fixing, indole-3-acetic acid (IAA) production, and phosphate solubilization ability. In addition, proteomic analysis and relative quantitative PCR revealed an elevated level of 4-hydroxyphenylacetate 3-monooxygenase (HPMO) in quinclorac-treated J5, suggesting that this enzyme may play an important role in Quinclorac remediation. This study showed that the J5 isolate could be exploited to not only assist in soil remediation due to Quinclorac residue issues but also promote tobacco growth.
Exploring Quinclorac resistance mechanisms in Echinochloa crus-pavonis from China
Pest Manag Sci 2021 Jan;77(1):194-201.PMID:32652760DOI:10.1002/ps.6007.
Background: Barnyardgrass (Echinochloa spp.) is a global weed in rice fields. Quinclorac is commonly used to control barnyardgrass. However, due to persistent use, Quinclorac resistance has evolved. We obtained quinclorac-susceptible (QS) and -resistant (QR1, QR2) lines from the progeny of a single resistant E. crus-pavonis for a resistance mechanism study. Results: Line QR1 exhibited resistance to high Quinclorac rates (up to 6400 g ha-1 ), whereas line QR2 exhibited a resistance/susceptibility segregation ratio of 3:1 at the field or lower rates (400, 100 g ha-1 ). Intriguingly, a lower level of 14 C-quinclorac metabolism and hence a higher level of 14 C-quinclorac translocation was observed in QR1 than QS plants. The basal expression levels of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase 2 (ACO2) genes did not differ significantly between the QR1 and QS lines. However, more expression of ACS and ACO genes was induced by Quinclorac treatment in QS than in QR1. Basal levels of β-cyanoalanine synthase (β-CAS) gene expression were similar in QS and QR1 plants, but a greater level of down-regulation was detected in QS than in QR1 plants after Quinclorac treatment. Conclusion: These results indicate QR plants are less responsive to Quinclorac than QS plants in terms of up-regulating Quinclorac metabolism and ethylene synthesis. Resistance in this E. crus-pavonis line is likely controlled by a single major gene, involving possibly an alteration in auxin signal perception/transduction to the ethylene biosynthesis pathway. The β-CAS is unlikely to play a major role in Quinclorac resistance in this particular population.
Phytoremediation of Quinclorac and tebuthiuron-polluted soil by green manure plants
Int J Phytoremediation 2021;23(5):474-481.PMID:33000969DOI:10.1080/15226514.2020.1825329.
Quinclorac and tebuthiuron are residual herbicides that may remain in the soil longer than for the cropping season. The objective of this research was to evaluate the use of green manure plants to remediate soils treated with Quinclorac and tebuthiuron. Soils were separately treated with 14C-quinclorac and 14C-tebuthiuron at 266.4 and 132 g ha-1, respectively. After 21 days, four green manure plants, namely Crotalaria spectabilis, Canavalia ensiformis, Stizolobium aterrimum, and Lupinus albus, were separately sown in the treated soils. Overall, all four species absorbed more 14C-tebuthiuron [C. ensiformes (22.49%), S. aterrimum (16.71%), L. albus (15%), and C. spectabilis (4.48%)] than 14C-quinclorac [C. ensiformis (13.44%), L. albus (10.02%), S. aterrimum (6.2%), and C. spectabilis (1.75%)]. Quinclorac translocation in all four plants was greater in young leaves compared to old leaves, cotyledons, or roots, and 14C-tebuthiuron translocation in all four plant species was greater in old leaves and cotyledons compared to young leaves or roots. Regardless of the differences in translocation between the two herbicides, the four green manure plants are capable to remediate soils that have been treated with Quinclorac and tebuthiuron. However, C. ensiformis is more efficient for the remediation of tebuthiuron-treated soil compared to the other plants.