Phosphatase Inhibitor Cocktail III (100× in DMSO)
Sample solution is provided at 25 µL, 10mM.
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial contains a 1 ml solution with the following components: 500 µM Cantharidin, 2.5 mM (-)-p-Bromotetramisole oxalate, and 1 µM Calyculin A form Discodermia calyx.
Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
|(-)-p-Bromolevamisole oxalate||373.2||2.5 mM||Alkaline phosphatases|
|Cantharidin||196.2||500 µM||protein phosphatase 2A(PP2A)|
|Calyculin A||1009.2||1 µM||protein phosphatases 1 and 2A(PP1 and PP2A)|