N-3-hydroxyoctanoyl-L-Homoserine lactone
(Synonyms: OHC8HSL) 目录号 : GC41575A C8 quorum-sensing lactone
Cas No.:192883-14-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Quorum sensing is a regulatory system used by bacteria for controlling gene expression in response to increasing cell density. Different quorum sensing molecules are produced at different times in bacterial population growth and have distinct cellular effects mediated through changes in gene expression. N-3-hydroxyoctanoyl-L-Homoserine lactone is a small diffusible signaling molecule secreted by various bacteria. This lactone can have activating or suppressing effects on gene expression and biofilm formation, respectively. N-3-hydroxyoctanoyl-L-Homoserine lactone is produced via lactonolysis from 3-oxooctanoyl-homoserine lactone, altering quorum sensing or contributing to quorum quenching.
| Cas No. | 192883-14-0 | SDF | |
| 别名 | OHC8HSL | ||
| Canonical SMILES | O=C1[C@@H](NC(CC(O)CCCCC)=O)CCO1 | ||
| 分子式 | C12H21NO4 | 分子量 | 243.3 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.1102 mL | 20.5508 mL | 41.1015 mL |
| 5 mM | 0.822 mL | 4.1102 mL | 8.2203 mL |
| 10 mM | 0.411 mL | 2.0551 mL | 4.1102 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Candidates of quorum sensing bacteria in activated sludge associated with N-acyl homoserine lactones
Chemosphere 2019 Dec;236:124292.PMID:31310968DOI:10.1016/j.chemosphere.2019.07.023.
This study aimed to explore candidates of microbial groups which is associated with quorum sensing in activated sludge. Activated sludge samples were collected from three wastewater treatment plants (WWTP) to analyze N-acyl homoserine lactone (AHL) by Fourier-transform mass spectrometry (FTMS) and 16S rRNA-based microbial community. Among activated sludge samples taken at 3 WWTPs in different seasons, 2 AHL species of N-3-hydroxyoctanoyl-L-Homoserine lactone and N-3-hydroxydecanoyl-l-homoserine lactone were detected in the range of ranged of 0.1 ng/L to 1.6 ng/L. The detected AHL species were not dependent on treatment systems nor seasons. From microbial community analysis, population abundance of one strain in Verrucomicrobia and two strains in Holophagaceae had high correlation with AHL concentration in activated sludge. Comamonadaceae had also moderately correlated population with AHL concentrations among quorum sensing bacteria reported previously.