Oxyberberin
(Synonyms: 氧化小檗碱; Oxyberberin; Berlambine; 8-Oxoberberine) 目录号 : GC36829
An alkaloid and metabolite of berberine with diverse biological activities
Cas No.:549-21-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Oxyberberine is an alkaloid and a metabolite of the alkaloid berberine that has been found in A. mexicana and has diverse biological activities.1,2,3 Oxyberberine is formed from berberine by gut microbiota.2 It reduces viability of SW480 colon cancer cells when used at concentrations ranging from 1 to 200 ?g/ml.1 Oxyberberine (50 and 100 mg/kg) increases the ratio of phosphorylated to unphosphorylated PI3K, Akt, and GSK3β in isolated rat pancreatic tissue and reduces rat blood glucose levels in an oral glucose tolerance test in a model of diabetes induced by streptozotocin .2 It also reduces inflammation and hepatosteatosis in a rat model of high-fat diet-induced non-alcoholic fatty liver disease (NAFLD).3
1.Singh, S., Verma, M., Malhotra, M., et al.Cytotoxicity of alkaloids isolated from Argemone mexicana on SW480 human colon cancer cell linePharm. Biol.54(4)740-745(2016) 2.Dou, Y., Huang, R., Li, Q., et al.Oxyberberine, an absorbed metabolite of berberine, possess superior hypoglycemic effect via regulating the PI3K/Akt and Nrf2 signaling pathwaysBiomed. Pharmacother.137111312(2021) 3.Li, Q.-P., Dou, Y.-X., Huabg, Z.-W., et al.Therapeutic effect of oxyberberine on obese non-alcoholic fatty liver disease ratsPhytomedicine85153550(2021)
| Cas No. | 549-21-3 | SDF | |
| 别名 | 氧化小檗碱; Oxyberberin; Berlambine; 8-Oxoberberine | ||
| Canonical SMILES | O=C(C1=C(OC)C(OC)=CC=C1C=C23)N2CCC(C3=C4)=CC5=C4OCO5 | ||
| 分子式 | C20H17NO5 | 分子量 | 351.35 |
| 溶解度 | Soluble in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8462 mL | 14.2308 mL | 28.4616 mL |
| 5 mM | 0.5692 mL | 2.8462 mL | 5.6923 mL |
| 10 mM | 0.2846 mL | 1.4231 mL | 2.8462 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
[Tissue Distribution of Alkaloids from Coptidis Rhizoma in Rats Determined by UPLC-MS]
Zhong Yao Cai 2016 Aug;.39(8):1849-53.PMID:30204391doi
Objective: To develop an UPLC-MS method for simultaneous determination of alkaloids from Coptidis Rhizoma in rat tissues, and to study the tissue distribution of alkaloids from Coptidis Rhizoma in rats. Methods: The samples were extracted with ethyl acetate, and analyzed by UPLC-MS with acetonitrile-0. 2% formic acid solution in a gradient elution mobile phase, the flow rate was 0. 2m L/min. Tetrahydropalmatine was used as an internal standard. The mass spectrometer was operated in selected reaction monitoring( SIM) mode with positive electrospray ionization, the transition were m/z 191. 904 /118. 973( noroxyhydrastinine), m/z 335. 877 /308. 072( 8-ocoptisine),m/z 351. 94 /294. 554( palmatine chloride),m/z 335. 94 /262. 112( epiberberine), m/z 337. 94 /322. 422( columbamine), m/z 319. 904 /292. 037( coptisine), m/z 355. 977 /192. 036( tetrahydropalmatine),m/z 335. 94 /320. 036( berberine hydrochloride),m/z 351. 94 /321. 995( Oxyberberin), m/z 337. 94 /322. 949( jatrorrhizine respectively). Results: Excellent linearity was observed in all alkaloids in their linear range( r & 0. 9901). The RSD of precision of the developed method was less than 15%,and the accuracy and stability were less than ± 15%,the extraction recovery was 72. 1% ~ 82. 9% with RSD less than 15%. Coptisine,epiberberine,berberine,jatrorrhizine,columbamine,palmatine were widely distributed in rat tissues. Noroxyhydrastinine,8-ocoptisine,Oxyberberin could only be determined in liver and heart or kidney. Conclusion: The established method is simple and accurate. Satisfactory results are obtained with applying this method to the tissue distribution study of alkaloids from Coptidis Rhizoma.