Home>>DiD perchlorate
DiD perchlorate 目录号 GC30543

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5mg
¥1,071.00
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Sample solution is provided at 25 µL, 10mM.

实验参考方法

Animal experiment:

1 to 5×105 cells per mL HSPCs are stained with 5 μM DiD perchlorate (DiD) or 7.5 μM DiI in PBS without serum for 10 min at 37°C, washed once in PBS and immediately injected into the tail vein of recipient mice. Unless stated differently, each imaged CD45.2 mouse receive 8,000 to 15,000 labelled CD45.1 HSPCs together with 3 to 5×105 CD45.2 supportive whole bone-marrow mononuclear cells to ensure survival[1].

References:

[1]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.

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Chemical Properties

Cas No. 127274-91-3 SDF Download SDF
别名 N/A
化学名 N/A
Canonical SMILES CCCCCCCCCCCCCCCCCCN1C2=C(C=CC=C2)C(C)(C)/C1=C\C=C\C=C\C3=[N+](C4=C(C=CC=C4)C3(C)C)CCCCCCCCCCCCCCCCCC.O=Cl(=O)([O-])=O
分子式 C61H99ClN2O4 分子量 959.9
溶解度 DMSO: 50 mg/mL (52.09 mM) 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

DiD perchlorate is a lipophilic cyanine dye.

Two weeks after injection of stained cells, a single, bright, DiD perchlorate (DiD)-positive cells located between 15 to 40 µm from the endosteum is found. Results reveal a progressive appearance of cell clusters of decreased dye intensity, consistent with the partitioning of DiD perchlorate label on cell division[1].

[1]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.