2,4-D (2,4-Dichlorophenoxyacetic acid)
(Synonyms: 2,4-二氯苯氧乙酸,2,4-Dichlorophenoxyacetic acid) 目录号 : GC60459
A plant growth regulator
Cas No.:94-75-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2,4-
1.Rahman, A., Bannigan, A., Sulaman, W., et al.Auxin, actin and growth of the Arabidopsis thaliana primary rootPlant J.50514-528(2007)
| Cas No. | 94-75-7 | SDF | |
| 别名 | 2,4-二氯苯氧乙酸,2,4-Dichlorophenoxyacetic acid | ||
| Canonical SMILES | O=C(O)COC1=CC=C(Cl)C=C1Cl | ||
| 分子式 | C8H6Cl2O3 | 分子量 | 221.04 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,Ethanol:PBS(pH 7.2) (1:1): 0.5 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5241 mL | 22.6203 mL | 45.2407 mL |
| 5 mM | 0.9048 mL | 4.5241 mL | 9.0481 mL |
| 10 mM | 0.4524 mL | 2.262 mL | 4.5241 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Insight into the mode of action of 2,4-Dichlorophenoxyacetic acid (2,4-D) as an herbicide
J Integr Plant Biol 2014 Feb;56(2):106-13.PMID:24237670DOI:10.1111/jipb.12131.
2,4-Dichlorophenoxyacetic acid (2,4-D) was the first synthetic herbicide to be commercially developed and has commonly been used as a broadleaf herbicide for over 60 years. It is a selective herbicide that kills dicots without affecting monocots and mimics natural auxin at the molecular level. Physiological responses of dicots sensitive to auxinic herbicides include abnormal growth, senescence, and plant death. The identification of auxin receptors, auxin transport carriers, transcription factors response to auxin, and cross-talk among phytohormones have shed light on the molecular action mode of 2,4-D as a herbicide. Here, the molecular action mode of 2,4-D is highlighted according to the latest findings, emphasizing the physiological process, perception, and signal transduction under herbicide treatment.
Microbial degradation of 2,4-Dichlorophenoxyacetic acid: Insight into the enzymes and catabolic genes involved, their regulation and biotechnological implications
Crit Rev Microbiol 2016;42(2):194-208.PMID:25058513DOI:10.3109/1040841X.2014.917068.
A considerable progress has been made to understand the mechanisms of biodegradation of 2,4-Dichlorophenoxyacetic acid (2,4-D). 2,4-D biodegradation pathway has been elucidated in many microorganisms including Cupriavidus necator JMP134 (previously known as Wautersia eutropha, Ralstonia eutropha and Alcaligenes eutrophus) and Pseudomonas strains. It generally involves the side chain removal of 2,4-D by α-ketoglutarate-dependent 2,4-D dioxygenase (tfdA) to form 2,4-dichlorophenol (2,4-DCP); hydroxylation of 2,4-DCP by 2,4-DCP hydroxylase (tfdB) to form dichlorocatechol; ortho or meta cleavage of dichlorocatechol by chlorocatechol 1,2-dioxygenase (tfdC) to form 2,4-dichloro-cis,cis-muconate; conversion of 2,4-dichloro-cis,cis-muconate to 2-chlorodienelactone by chloromuconate cycloisomerase (tfdD); conversion of 2-chlorodienelactone to 2-chloromaleylacetate by chlorodienelactone hydrolase (tfdE) and, finally, conversion of 2-chloromaleylacetate to 3-oxoadepate via maleylacetate by chloromaleylacetate reductase and maleylacetate reductase (tfdF), respectively, which is funnelled to the tricarboxylic acid cycle. The latest review on microbial breakdown of 2,4-D, other halogenated aromatic pesticides, and related compounds was compiled by Haggblom, however, a considerable progress has been made in this area of research since then. Thus, this review focuses on the recent advancement on 2,4-D biodegradation, the enzymes, and genes involved and their biotechlogical implications.
Adsorption mechanism of 2,4-Dichlorophenoxyacetic acid onto nitric-acid-modified activated carbon fiber
Environ Technol 2018 Apr;39(7):895-906.PMID:28379070DOI:10.1080/09593330.2017.1316318.
Adsorption by carbon materials is one of the relatively fast methods in present research, which is widely used in emergency events. Activated carbon fiber (ACF) modified by nitric acid (N-ACF) was studied in this research to determine the adsorption performance for 2,4-Dichlorophenoxyacetic acid (2,4-D). Subsequently, influence factors, adsorption isotherm models, kinetics and thermodynamic were investigated in a batch system to realize this adsorption. Experimental results showed that ACF modified by 0.1M nitric acid had a better removal ability than 2,4-D. Removal rate of 2,4-D by N-ACF was greatly influenced by pH with the optimum pH at 2. The superiority of the Langmuir isotherm model in describing the adsorption equilibrium was revealed by correlation coefficients R2 (R2 ≥ 0.997). Furthermore, adsorption kinetics was well described by pseudo-second-order model. The results of thermodynamic showed that adsorption was a spontaneous, endothermic process with randomness increasing. Additionally, surface structure properties of adsorbent were characterized by Scanning electron microscopy, Fourier transform infrared spectroscopy, Specific surface area analysis of Brunauer, Emmett and Teller and Boehm's titration. It turned out that the micropore structure and functional groups on N-ACF all can contribute to the removal of 2,4-D.
Preparation of UiO-66-NH2 and UiO-66-NH2/sponge for adsorption of 2,4-Dichlorophenoxyacetic acid in water
Ecotoxicol Environ Saf 2020 May;194:110440.PMID:32169729DOI:10.1016/j.ecoenv.2020.110440.
MOFs are usually used as efficient adsorbents to remove specific pollutants in water. However, because of their poor water stability relatively small particle size, their application in adsorbing and removing pollutants from water is limited. In this paper, with nitrile rubber sponge as the substrate, UiO-66-NH2/sponge composites were firstly in-situ synthesized and systematically evaluated UiO-66-NH2 as an adsorbent to remove 2,4-Dichlorophenoxyacetic acid from water. This composite could not only remain the adsorption capacity for 2,4-Dichlorophenoxyacetic acid of UiO-66-NH2, but also was much more convenient for separation after the adsorption compared to UiO-66-NH2. In addition, the mechanism of the adsorption of UiO-66-NH2 for 2,4-Dichlorophenoxyacetic acid were discussed in detail. Electrostatic interaction between UiO-66-NH2 and 2,4-Dichlorophenoxyacetic acid was the main adsorption mechanism. The adsorption was mainly suitable for Langmuir isotherm models, and its maximum adsorption capacity of 2,4-Dichlorophenoxyacetic acid was 72.99 mg g-1.
2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader
PLoS One 2018 Jun 22;13(6):e0199677.PMID:29933393DOI:10.1371/journal.pone.0199677.
The study reports the response to herbicide of the 2,4-Dichlorophenoxyacetic acid (2,4-D)-degrading fungal strain Umbelopsis isabellina. A comparative analysis covered 41 free amino acids as well as 140 lipid species of fatty acids, phospholipids, acylglycerols, sphingolipids, and sterols. 2,4-D presence led to a decrease in fungal catalase activity, associated with a higher amount of thiobarbituric acid-reactive substances (TBARS). Damage to cells treated with the herbicide resulted in increased membrane permeability and decreased membrane fluidity. Detailed lipidomic profiling showed changes in the fatty acids composition such as an increase in the level of linoleic acid (C18:2). Moreover, an increase in the phosphatidylethanolamine/phosphatidylcholine ratio was observed. Analysis of fungal lipid profiles revealed that the presence of 2,4-D was accompanied by the accumulation of triacylglycerols, a decrease in ergosterol content, and a considerable rise in the level of sphingolipid ceramides. In the exponential phase of growth, increased levels of leucine, glycine, serine, asparagine, and hydroxyproline were found. The results obtained in our study confirmed that in the cultures of U. isabellina oxidative stress was caused by 2,4-D. The herbicide itself forced changes not only to membrane lipids but also to neutral lipids and amino acids, as the difference of tested compounds profiles between 2,4-D-containing and control samples was consequently lower as the pesticide degradation progressed. The presented findings may have a significant impact on the basic understanding of 2,4-D biodegradation and may be applied for process optimization on metabolomic and lipidomic levels.