Ubiquitin G76A Human
目录号 : GP24938Ubiquitin G76A Human Recombinant
Sample solution is provided at 25 µL, 10mM.
Purity | Greater than 95% as determined by SDS-PAGE. | Source | Escherichia Coli. |
Phycical Appearance | Sterile Filtered clear solution. | Shipping Condition | Shipped with Ice Packs. |
Synonyms | Ubiquitin; Ribosomal Protein S27a; CEP80; UBA80; UBCEP1; UBCEP80; HUBCEP80; RPS27A; Ubiquitin G76A. | ||
Stability | Store vial at -20°C to -80°C . When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles. | ||
Formulation | Diluted in PBS plus 5% glycerol. |
The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins.Ubiquitination occurs through sequential steps catalyzed by activating (E1), conjugating (E2), and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ubбпs C-terminal glycine residue (G76) and a lysine residue of the target protein, although N-terminal ubiquitination is also known.Outcomes of this modification include destabilization of the conjugated protein, altered protein trafficking and functional modulation.After targeting the protein for specific localizations, ubiquitin is released from the substrate by deubiquitinating enzymes.A mutant ubiquitin, having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process.As consequence, the Ub derivative becomes irreversibly conjugated to protein, shifting the equilibrium between the bound and unbound form in the direction of conjugation, at the expense of the free form.
Store vial at -20°C to -80°C . When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles.