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TOP1 Bovine

目录号 : GP22310

DNA Topoisomerase-I Bovine

TOP1 Bovine Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
20μg
¥2,030.00
5-10工作日
1mg
¥54,600.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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产品描述

Bovine DNA Topoisomerase-I purified from bovine tissues by proprietary chromatographic techniques.

Product Data

Purity Greater than 70% as determined by SDS-PAGE. Source Bovine tissues.
Phycical Appearance Shipping Condition Shipped with Ice Packs.
Synonyms DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.
Formulation TOP1 is supplied at a concentration of 1mg/1ml in 16mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.

Introduction

TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.

Applications

Western blot with Scleroderma positive patient sera.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.