Home>>Proteins>> Enzymes>> Isomerase>>TOP1 70kDa Human

TOP1 70kDa Human

目录号 : GP22309

DNA Topoisomerase-I 70kDa Recombinant Human

TOP1 70kDa Human Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
20μg
¥2,030.00
5-10工作日
1mg
¥54,600.00
5-10工作日

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

产品描述

Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.

Product Data

Purity Greater than 80% as determined by SDS-PAGE. Source Sf9 insect cells.
Phycical Appearance Shipping Condition Shipped with Ice Packs.
Synonyms DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.
Formulation TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.

Introduction

TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.

Applications

Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.