Tetrahydroepiberberine
(Synonyms: 四氢表小檗碱) 目录号 : GC39098An alkaloid with diverse biological activities
Cas No.:38853-67-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
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Tetrahydroepiberberine is an alkaloid originally isolated from P. rhoeas that has diverse biological activities.1,2 It is active against the fungus C. albicans (MIC = 8 ?g/ml), as well as the bacteria E. coli, P. aeruginosa, P. mirabilis, K. pneumoniae, A. baumannii, S. aureus, and B. subtilis (MICs = 32-64 ?g/ml, respectively).2 Tetrahydroepiberberine (16 ?g/ml) inhibits the cytopathogenic effect of parainfluenza-3 (PI-3) virus in Vero cells. It decreases the viability of HaCaT keratinocytes (IC50 = 150 ?M).1
1.Hijazi, M.A., Aboul-Ela, M., Bouhadir, K., et al.Cytotoxic activity of alkaloids from Papaver rhoeas growing in LebanonRec. Nat. Prod.11(2)211-216(2017) 2.Orhana, I., Oz?elik, B., Karao?lu, T., et al.Antiviral and antimicrobial profiles of selected isoquinoline alkaloids from Fumaria and Corydalis speciesZ. Naturforsch. C. J. Biosci.62(1-2)19-26(2007)
Cas No. | 38853-67-7 | SDF | |
别名 | 四氢表小檗碱 | ||
Canonical SMILES | COC1=CC2=C(C=C1OC)CCN3C2CC(C=CC4=C5OCO4)=C5C3 | ||
分子式 | C20H21NO4 | 分子量 | 339.39 |
溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.9465 mL | 14.7323 mL | 29.4646 mL |
5 mM | 0.5893 mL | 2.9465 mL | 5.8929 mL |
10 mM | 0.2946 mL | 1.4732 mL | 2.9465 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Simultaneous quantification of 11 isoquinoline alkaloids in Corydalis impatiens (Pall.) Fisch by HPLC
J Sep Sci 2013 Jul;36(13):2090-5.PMID:23610009DOI:10.1002/jssc.201300036.
Isoquinoline alkaloids are the primary active ingredients of Corydalis, but an analytical method for quality assessment of the active ingredients in Corydalis impatiens (Pall). Fisch has not been reported. A new, simple, and multiple-component quantification method was developed for the simultaneous quantification of 11 isoquinoline alkaloids including capnoidine, chelianthifoline, bicuculline, protopine, isoapocavidine, apocavidine, cavidine, Tetrahydroepiberberine, ochotensimine, tetrahydrocoptisine, and tetrahydrocorysamine in C. impatiens. Separation of the isoquinoline alkaloids was performed on a RP C18 column (150 × 4.6 mm, 5 μm) with potassium dihydrogen phosphate buffer (pH 2.5, adjusted by phosphoric acid)/acetonitrile (53:47, v/v) containing 0.3% sodium dodecyl sulfonate. The flow rate and detection wavelength were set at 1 mL/min and 295 nm, respectively. Full validation of the assay was carried out including linearity, precision, accuracy, stability, LOD, and limit of quantitation. All calibration curves showed a good linear relationship (r > 0.999) in test range. The results demonstrated that the developed method was reliable, rapid, and specific. Six batches of C. impatiens samples from different sources were determined using the established method. The contents of alkaloids ranged from 11.68 to 351.83 μg/g. This method can be applied for quality evaluation and control of C. impatiens. Eleven isoquinoline alkaloids were first reported on simultaneous determination with HPLC.