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T4 Polynucleotide Kinase

(Synonyms: T4多聚核苷酸激酶) 目录号 : GC26293

T4 Polynucleotide Kinase,简称T4 PNK,中文名称T4多聚核苷酸激酶,是一种多聚核苷酸5'羟基激酶,

T4 Polynucleotide Kinase Chemical Structure

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100U
¥220.00
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500U
¥714.00
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Sample solution is provided at 25 µL, 10mM.

Description

T4 Polynucleotide Kinase, abbreviated as T4 PNK, is a polynucleotide 5 'hydroxy kinase that catalyzes ATP γ The phosphate group is transferred to the 5 'hydroxyl group of single or double stranded DNA, RNA, oligonucleotides, or single nucleotides with a 3' phosphate group. Other NTPs can also produce the same reaction: 5 '- OH+NTP → 5' - P+NDP.

T4 Polynucleotide Kinase,简称T4 PNK,中文名称T4多聚核苷酸激酶,是一种多聚核苷酸5'羟基激酶,可以催化ATP的γ位磷酸基团向单链或双链DNA、RNA、寡核苷酸或带有3'磷酸基团的单核苷酸的5'羟基转移。其他NTP也可产生相同的反应:5'-OH + NTP → 5'-P + NDP。

实验参考方法

操作流程

反应体系的设置:

1) 溶解并混匀PCR反应所需的各种溶液。并放置于冰浴上或冰盒内。建议反应PCR液体分装使用,避免反复冻融。

2) 参考下表设置的反应,建议PCR反应体系的配置在冰浴或在冰盒上进行:

试剂 50 μl 反应体系
T4 Polynucleotide Kinase (10 U/ul)  1-2 μl
5×T4 PNK Buffer 10 μl
待磷酸化DNA
γ-[32P]-ATP/ATP(10mM)  5 μl
T4PNK、模板、ATP可根据实验具体调整用量,不足50 μl 用RNaseFree-H2O 补足
体积 50 μl

3) 37°C温育30分钟。

产品组成

Components GC26293-0500 GC26293-1000
T4 Polynucleotide Kinase(10 U/ul) 50 µl 100 µl
5X T4 PNK Buffer 500 µl 1 ml

产品文档

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