SYTO 9 (20x in DMSO) Nucleic Acid Dyes for PCR

SYTO9 Green fluorescent Nucleic Acid Stain (SVTO9 Green fluorescent Nucleic Acid Stain) is an excellent counterstain for nuclei and chromosomes and is highly permeable to cell membranes. SYTO 9 binds DNA (and RNA) with high affinity. Once bound, it shows a significantly enhanced fluorescence signal. It is excited by blue light (maximum emission wavelength 485nm) and emits green fluorescence (maximum excitation is 498nm (DNA) and 501nm (RNA) respectively). . Because SYTO 9 can penetrate well into prokaryotic and eukaryotic cell membranes, it is commonly used as a nuclear counterstain (especially bacterial cells). It is often used in combination with dead cell nuclear counterstains (such as propidium iodide PI). Live cell/dead bacterial staining.

What is more noteworthy is that SYTO 9, as a sensitive DNA-binding dye, exhibits many excellent characteristics in RT-PCR, including: producing highly reproducible DNA melting curves under a wide range of dye concentrations, extremely low PCR inhibition rate, high signal-to-noise ratio and high sensitivity make it an ideal SYBR Green I alternative dye for RT-PCR, DNA melting curve analysis, and loop-mediated isothermal amplification (LAMP) experiments [3- 5].
This product is 20×SYTO 9 nucleic acid dye dissolved in DMSO, reaching PCR level. When using it, you only need to add the amount according to the system to make the final concentration 1×.

Storage and transportation methods
Storage: Store at 2-8°C away from light, or at -20°C. Valid for 2 years.
Shipping: Ice pack shipping.

SYTO 9绿色荧光核酸染料 (SVTO9 Green fluorescent Nucleic Acid Stain)，是一款优秀的细胞核和染色体复染剂，具有高度细胞膜渗透性。SYTO 9高亲和结合DNA(以及RNA)，一旦结合后呈现明显增强的荧光信号，用蓝光激发(最大发射波长485nm)，发绿色荧光(最大激发分别是498nm(DNA)和501nm(RNA))。SYTO 9由于能很好的渗透进入原核和真核细胞膜，普遍用作核复染剂(特别是细菌细胞)，常常与死细胞核复染剂(比如:碘化丙啶PI)联合使用，用于活细莹/死细菌染色。

更值得关注的是， SYTO 9作为一款灵敏的DNA 结合染料，在RT-PCR 中，表现出许多优异的特征，包括：在宽广的染料浓度下产生高度可重复的DNA 熔解曲线，极低 的PCR抑制率，高信噪比和高灵敏度，使其成为一款理想的SYBR Green I替代染料，用于RT-PCR、DNA 熔解曲线分析、以及环介导等温扩增 (LAMP) 实验[3-5]。
本品为溶于DMSO 的20×SYTO 9核酸染料，达PCR 级别，使用时仅需根据体系加量使其终浓度为1×即可。

保存与运输方法

保存：2-8℃避光保存，也可置于-20℃保存，2年有效。

运输：冰袋运输。