Sulforhodamine B sodium salt (Acid Red 52)
(Synonyms: 磺酰罗丹明B,Acid Red 52; Kiton Red 620) 目录号 : GC30365
An aminoxanthene dye
Cas No.:3520-42-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.80%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1]. |
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References: [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. |
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Sulforhodamine B is an aminoxanthene dye. It binds basic amino acid residues under mild acidic conditions, which can be quantified by colorimetric detection at 510 nM, and is commonly used for cell density quantification in cytotoxicity screening.1,2
1.Skehan, P., Storeng, R., Scudiero, D., et al.New colorimetric cytotoxicity assay for anticancer-drug screeningJ. Natl. Cancer Inst.82(13)1107-1112(1990) 2.Vichai, V., and Kirtikara, K.Sulforhodamine B colorimetric assay for cytotoxicity screeningNat. Protoc.1(3)1112-1116(2006)
| Cas No. | 3520-42-1 | SDF | |
| 别名 | 磺酰罗丹明B,Acid Red 52; Kiton Red 620 | ||
| Canonical SMILES | O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)([O-])=O.[Na+] | ||
| 分子式 | C27H29N2NaO7S2 | 分子量 | 580.65 |
| 溶解度 | DMSO : 50 mg/mL (86.11 mM);Water : 20 mg/mL (34.44 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7222 mL | 8.611 mL | 17.2221 mL |
| 5 mM | 0.3444 mL | 1.7222 mL | 3.4444 mL |
| 10 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。