Sulfamethazine-D4
(Synonyms: 氘代磺胺二甲基嘧啶,Sulfadimidine-d4; Sulfadimerazine-d4) 目录号 : GC61303
An internal standard for the quantification of sulfamethazine
Cas No.:1020719-82-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Sulfamethazine-d4 is intended for use as an internal standard for the quantification of sulfamethazine by GC- or LC-MS. Sulfamethazine is a sulfonamide antibiotic.1,2 It inhibits dihydropteroate synthase (DHPS; IC50 = 5.7 ?M for the T. gondii enzyme). Sulfamethazine is active against A. pleuropneumoniae (MIC = 32 ?g/ml) and enhances the antibacterial activity of trimethoprim against E. coli.3,4 It has been detected in environmental water samples.5,6 Formulations containing sulfamethazine have been used in the treatment of bacterial infections in livestock.
1.Allegra, C.J., Boarman, D., Kovacs, J.A., et al.Interaction of sulfonamide and sulfone compounds with Toxoplasma gondii dihydropteroate synthaseJ. Clin. Invest.85(2)371-379(1990) 2.Salmon, S.A., Watts, J.L., Case, C.A., et al.Comparison of MICs of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the United States, Canada, and DenmarkJ. Clin. Microbiol.33(9)2435-2444(1995) 3.Mengelers, M.J., Hougee, P.E., Janssen, L.H., et al.Structure-activity relationships between antibacterial activities and physicochemical properties of sulfonamidesJ. Vet. Pharmacol. Ther.20(4)276-283(1997) 4.Peng, F.-J., Ying, G.-G., Liu, Y.-S., et al.Joint antibacterial activity of soil-adsorbed antibiotics trimethoprim and sulfamethazineSci. Total Environ.506-50758-65(2015) 5.Washington, M.T., Moorman, T.B., Soupir, M.L., et al.Monitoring tylosin and sulfamethazine in a tile-drained agricultural watershed using polar organic chemical integrative sampler (POCIS)Sci. Total. Environ.612358-367(2017) 6.López-Serna, R., Petrovi?, M., and Barceló, D.Direct analysis of pharmaceuticals, their metabolites and transformation products in environmental waters using on-line TurboFlow? chromatography-liquid chromatography-tandem mass spectrometryJ. Chomatogr. A.1252115-129(2012)
| Cas No. | 1020719-82-7 | SDF | |
| 别名 | 氘代磺胺二甲基嘧啶,Sulfadimidine-d4; Sulfadimerazine-d4 | ||
| Canonical SMILES | O=S(C1=C([2H])C([2H])=C(N)C([2H])=C1[2H])(NC2=NC(C)=CC(C)=N2)=O | ||
| 分子式 | C12H10D4N4O2S | 分子量 | 282.35 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5417 mL | 17.7085 mL | 35.417 mL |
| 5 mM | 0.7083 mL | 3.5417 mL | 7.0834 mL |
| 10 mM | 0.3542 mL | 1.7709 mL | 3.5417 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Antibiotic standards stored as a mixture in water: methanol are unstable at various temperatures irrespective of pH and glass container silanization
Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2022 Jan;39(1):61-73.PMID:34854803DOI:10.1080/19440049.2021.1982150
It is well-established that antibiotics stored individually at their optimal pH and in appropriate solvents are stable over time. However, limited information exists on the stability of antibiotics from multiple classes when prepared and stored as a mixture prior to multiresidue analysis by mass spectrometry. This study tested the stability of antibiotic standard mixtures from eight classes [amphenicols, tetracyclines, sulfonamides, quinolones, macrolides, β-lactams, lincosamides and miscellaneous (i.e., trimethoprim)] in relation to the water:methanol ratio, presence of sodium hydroxide base (to solubilise quinolones), storage temperature, and container type including plain and silanized glass vials. Antibiotics were analysed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. Several antibiotics, mainly quinolones, tetracyclines and macrolides, were unstable when stored as mixtures for one week regardless of the water:methanol ratio, storage temperature (4, -20 or -80 °C) and presence/absence of sodium hydroxide. Silanization of glassware improved the storage stability of quinolones and macrolides but reduced the stability of tetracyclines and other antibiotics including florfenicol amine, penicillin G, erythromycin and sulfadiazine. Our results show that several antibiotics in water:methanol are unstable when stored as a mixture and suggest a limited advantage of using base or silanized glass vials for the preparation and storage of antibiotic standards mixed together. Freshly prepared antibiotic standard mixtures are recommended for multi-residue quantitation of antibiotics.Abbreviations AMOX: amoxicillin; AMP: ampicillin; AZ: azithromycin; CAP: chloramphenicol; CE: collision energy; CTC: chlortetracycline; CIP: ciprofloxacin; DOX: doxycycline; ENO: enoxacin; ENRO: enrofloxacin; ERYTH: erythromycin; FF: florfenicol; FFA: florfenicol amine; FLU: flumequine; HDPE: high-density polyethylene; LC-MS/MS: liquid chromatography-tandem mass spectrometry; LIN: lincomycin; MRM: multiple reaction monitoring; NOR: norfloxacin; OFL-D3: ofloxacin-D3; OXO: oxolinic acid; OTC: oxytetracycline; PEN-G: penicillin G; PEN-V: penicillin V; ROX: roxithromycin; SDM: sulfadimethoxine; SDZ: sulfadiazine; SMX: sulfamethoxazole; SMZ-D4: Sulfamethazine-D4; SSZ: sulfasalazine; TC: tetracycline; TAP: thiamphenicol; TILM: tilmicosin; TRIM: trimethoprim; TL: tolerance limit; VIRG: virginiamycin; UPLC-MS/MS: ultra-high pressure liquid chromatography-tandem mass spectrometry.