Substance P
(Synonyms: P物质; Neurokinin P) 目录号 : GC15649
Substance P作为一种重要的神经肽类神经递质，能够刺激磷脂酰肌醇代谢，EC50值为0.36nM。
Cas No.:33507-63-0
Sample solution is provided at 25 µL, 10mM.
Substance P, an important neuropeptide neurotransmitter, stimulates phosphatidylinositol turnover with an EC50 value of 0.36nM[1]. Substance P has been widely used in the research of pain regulation, emotion regulation and bone metabolism regulation[2-3].
In vitro, Substance P (10nM) treatment induced Akt phosphorylation at Ser-473 in a time-dependent manner and persisted over the 60-minute incubation period in colonic epithelial NCM460 colonocytes[4]. Treatment with 10nM Substance P for 8 hours inhibited tamoxifen (10μM; 8h) induced cell death of NCM460 colonocytes, and cell proliferation was stimulated by treatment with 10nM Substance P for 8h in the absence of tamoxifen[4]. Treatment of buffalo fetal fibroblasts (10nM) with Substance P alone resulted in significant cell proliferation and migration in both horizontal and vertical directions within 24 hours[5].
In vivo, topical application of Substance P (32μg/day per wound dissolved in saline) for 10 days improved wound healing and reduced wound size in a diabetic mouse model[6]. A single dose of topical Substance P treatment (0.5μg/wound) promoted wound closure and epithelial regeneration within 14 days, thereby enhancing wound healing in a skin full-thickness wound model of mouse[7].
References:
[1] Torrens Y, Beaujouan J C, Saffroy M, et al. Substance P receptors in primary cultures of cortical astrocytes from the mouse[J]. Proceedings of the National Academy of Sciences, 1986, 83(23): 9216-9220.
[2] Li F X Z, Xu F, Lin X, et al. The role of substance P in the regulation of bone and cartilage metabolic activity[J]. Frontiers in Endocrinology, 2020, 11: 77.
[3] Humes C, Sic A, Knezevic N N. Substance P’s impact on chronic pain and psychiatric conditions—a narrative review[J]. International journal of molecular sciences, 2024, 25(11): 5905.
[4] Koon H W, Zhao D, Zhan Y, et al. Substance P mediates antiapoptotic responses in human colonocytes by Akt activation[J]. Proceedings of the National Academy of Sciences, 2007, 104(6): 2013-2018.
[5] Kant V, Mahapatra P S, Gupta V, et al. Substance P, a neuropeptide, promotes wound healing via neurokinin-1 receptor[J]. The International Journal of Lower Extremity Wounds, 2023, 22(2): 291-297.
[6] Leal E C, Carvalho E, Tellechea A, et al. Substance P promotes wound healing in diabetes by modulating inflammation and macrophage phenotype[J]. The American journal of pathology, 2015, 185(6): 1638-1648.
[7] Kumar S, Tan Y, Berthiaume F. Neuropeptide substance p enhances skin wound healing in vitro and in vivo under hypoxia[J]. Biomedicines, 2021, 9(2): 222.
Substance P作为一种重要的神经肽类神经递质，能够刺激磷脂酰肌醇代谢，EC50值为0.36nM[1]。Substance P已被广泛应用于疼痛调节、情绪调控及骨代谢研究领域[2-3]。
在体内，10nM浓度的Substance P处理可诱导结肠上皮NCM460细胞中Akt蛋白Ser-473位点的磷酸化，且这种磷酸化作用呈时间依赖性，在60分钟孵育期内持续存在[4]。使用10nM浓度的Substance P处理8小时能抑制他莫昔芬（10μM；8小时）诱导的NCM460细胞死亡，而在无他莫昔芬条件下，10nM 浓度的Substance P处理8小时刺激细胞增殖[4]。单独使用10nM浓度的Substance P处理水牛胎儿成纤维细胞24小时，可显著促进细胞在水平和垂直方向上的增殖与迁移[5]。
在体外，局部施用Substance P（32μg/day/伤口，溶于生理盐水）连续10天能改善糖尿病小鼠模型的伤口愈合并缩小创面面积[6]。单次局部施用Substance P（0.5μg/伤口）治疗可在14天内促进小鼠皮肤全层缺损模型的伤口闭合和上皮再生，从而加速创面愈合[7]。
| Cell experiment [1]: | |
Cell lines | NCM460-NK-1R cells |
Preparation Method | NCM460-NK-1R cells were plated on chamber slides and grown to 50% confluence. Cells were pretreated with Akt inhibitor V (40μM) for 30min and then treated with tamoxifen (10μM) in the presence or absence of Substance P (10nM) for 8h at 37°C. Cells were fixed in 4% paraformaldehyde for 25min and then permeabilized with 0.2% Triton X-100 solution in PBS for 5min. Slides were processed for TUNEL assay. |
Reaction Conditions | 10μM; 8h |
Applications | Substance P treatment inhibited tamoxifen-induced cell death and apoptosis of NCM460-NK-1R cells. |
| Animal experiment [2]: | |
Animal models | C57BL/6J WT mice |
Preparation Method | 8 mice were injected with streptozotocin (50mg/kg in 0.1mol/L citrate buffer) by i.p. route once daily for 5 consecutive days to induce diabetes. Blood glucose level of >250mg/dL was used as an inclusion criteria for diabetic mice. Wound experiments were done 8 weeks after diabetes induction. NPH insulin (0.1 to 0.2 units) was administered as needed to prevent weight loss. Animals were anesthetized with i.p. ketamine (100mg/kg) and i.p. Xylazine (10mg/kg). The hair from the back of the mice was shaved, and two, 6-mm full-thickness excisional wounds were biopsied. Tracing of the wound area was done on acetate paper every day to monitor the rate of wound closure up to 10 days after wounding. The wound size was analyzed with ImageJ version 1.46r. Data are presented as percentage of the original wound (day 0). One wound was treated with Substance P (32μg per wound in saline) daily and the other wound was treated with saline daily until day of euthanasia. Animals were sacrificed at day 10 after wounding. |
Dosage form | 32μg/day per wound dissolved in saline for 10 days; topical application |
Applications | Substance P treatment improved wound healing and reduced wound size in a diabetic mouse model. |
References: | |
| Cas No. | 33507-63-0 | SDF | |
| 别名 | P物质; Neurokinin P | ||
| 分子式 | C63H98N18O13S | 分子量 | 1347.6 |
| 溶解度 | Soluble to 1 mg/ml in sterile water | 储存条件 | Desiccate at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.7421 mL | 3.7103 mL | 7.4206 mL |
| 5 mM | 0.1484 mL | 0.7421 mL | 1.4841 mL |
| 10 mM | 0.0742 mL | 0.371 mL | 0.7421 mL |
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