Spermine NONOate
(Synonyms: 精胺氮氧化加合物) 目录号 : GC13694A nitric oxide donor
Cas No.:136587-13-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Spermine NONOate is a diazeniumdiolate nitric oxide donor [1].
Nitric oxide (NO) increases intracellular guanosine 3' 5' cyclic monophosphate and activates guanylate cyclase, which then cause relaxation of vascular smooth muscle [1].
Spermine NONOate is a diazeniumdiolate nitric oxide donor. In rat pulmonary artery rings, spermine NONOate (100 μM) increased guanosine 3'5' cyclic monophosphate (cyclic GMP) by 35 fold and caused cyclic GMP-independent relaxation by activation of sarco-endoplasmic reticulum Ca2+-ATPase, Na+/K+-ATPase and calcium-activated potassium channels [1]. Spermine NONOate inhibited human low-density lipoprotein (LDL) oxidation induced by the copper(II) sulfate [2].
In rats with bilateral uterine horn injury, spermine NONOate (1 mg) inhibited the formation of adhesions. Also, The N,O-carboxymethylchitosan (NOCC) plus spermine NONOate group had a lower severity score and extent score than the spermine NONOate group. These results suggested that spermine NONOate reduced the postoperative adhesions [3].
References:
[1]. Homer KL, Wanstall JC. Cyclic GMP-independent relaxation of rat pulmonary artery by spermine NONOate, a diazeniumdiolate nitric oxide donor. Br J Pharmacol, 2000, 131(4): 673-682.
[2]. Goss SP, Hogg N, Kalyanaraman B. The antioxidant effect of spermine NONOate in human low-density lipoprotein. Chem Res Toxicol, 1995, 8(5): 800-806.
[3]. Duran B, Ak D, Cetin A, et al. Reduction of postoperative adhesions by N,O-carboxymethylchitosan and spermine NONOate in rats. Exp Anim, 2003, 52(4): 267-272.
| Cas No. | 136587-13-8 | SDF | |
| 别名 | 精胺氮氧化加合物 | ||
| 化学名 | N'-(3-aminopropyl)-N'-(4-((3-aminopropyl)amino)butyl)-N-hydroxynitrous hydrazide | ||
| Canonical SMILES | ON(N=O)N(CCCN)CCCCNCCCN | ||
| 分子式 | C10H26N6O2 | 分子量 | 262.35 |
| 溶解度 | aqueous buffers: >100 mg/ml (per Rao) | 储存条件 | Store at -80°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8117 mL | 19.0585 mL | 38.117 mL |
| 5 mM | 0.7623 mL | 3.8117 mL | 7.6234 mL |
| 10 mM | 0.3812 mL | 1.9059 mL | 3.8117 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。