Spectinomycin (hydrochloride hydrate)
(Synonyms: 奇霉素；大观霉素；盐酸大观霉素五水合物; Spectinomycin hydrochloride hydrate) 目录号 : GC13981
Spectinomycin是一种广谱类抗生素，可抑制多种革兰氏阳性菌和革兰氏阴性菌的生长。
Cas No.:22189-32-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Animal experiment: |
Rats: Spectinomycin (10 mg/kg) is administered intravenously to rats. Serial blood samples (approx. 250 μL) are collected pre-dose, and at 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, 12.0, 24.0, 36.0 and 48.0 h post-dose. Plasma is separated immediately by centrifugation (10,000g for 5 min at 4°C) and stored at −80°C until analysis. Urine samples are collected at an interval of 0-6, 6-12, 12-24, 24-36 and 36-48 h post-dose and stored at −80°C until analysis[4]. |
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References: [1]. Davies J, et al. Inhibition of protein synthesis by spectinomycin. Science. 1965 Sep 3;149(3688):1096-8. |
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Spectinomycin is a broad-spectrum antibiotic that inhibits the growth of a variety of Gram-positive and Gram-negative bacteria. Spectinomycin is also a non-competitive inhibitor of TD intron RNA with a Ki value of 7.2 mM [1].
Spectinomycin is an aminocyclic alcohol antibiotic produced by S. spectabilis. It inhibits microbial protein synthesis by binding to the 30S ribosomal subunit and protects the N-7 position of G1064 nucleotide on E. coli 16S rRNA from dimethyl sulfate. base. Spectinomycin binds to this position and is thought to stabilize helix 34, thereby inhibiting the binding of elongation factor G, preventing the transfer of peptide-tRNA from the A site to the P site of the ribosome, thereby inhibiting protein synthesis [2]. Mutations in the ribosomal protein S5 gene can prevent the binding of Spectinomycin and contribute to bacterial resistance [3].
In cell culture experiments, the recommended concentration of Spectinomycin is 7.5-20 μg/mL.
Spectinomycin是一种广谱类抗生素，可抑制多种革兰氏阳性菌和革兰氏阴性菌的生长。Spectinomycin也是TD内含子RNA的非竞争性抑制剂，Ki值为7.2 mM[1]。
Spectinomycin是由S. spectabilis产生的氨基环醇类抗生素，通过与30S核糖体亚基结合抑制微生物蛋白质合成，并保护大肠杆菌16S rRNA上G1064核苷酸的N-7位置不被二甲基硫酸甲基化。Spectinomycin结合于该位置，被认为稳定了34号螺旋，从而抑制了伸长因子G的结合，阻止了肽-tRNA从核糖体A位点转移到P位点，从而抑制蛋白合成[2]。核糖体蛋白S5基因的突变能够阻止Spectinomycin的结合，有助于细菌的耐药性[3]。
在细胞培养实验中，Spectinomycin的建议浓度为7.5-20μg/mL。
References:
[1] Khan EA, et al. Safety evaluation study of lincomycin and spectinomycin hydrochloride intramuscular injection in chickens. Toxicol Rep. 2022 Jan 29;9:204-209.
[2] Zimmerman J M, Maher L J. In vivo selection of spectinomycin‐binding RNAs[J]. Nucleic acids research, 2002, 30(24): 5425-5435.
[3] Kehrenberg, C., and Schwarz, S. Mutations in 16S rRNA and ribosomal protein S5 associated with high-level spectinomycin resistance in Pasteurella multocida. Antimicrob. Agents Chemother. 51(6), 2244-2246 (2007).
| Cas No. | 22189-32-8 | SDF | |
| 别名 | 奇霉素；大观霉素；盐酸大观霉素五水合物; Spectinomycin hydrochloride hydrate | ||
| 化学名 | (5aR,9aR,10aS)-decahydro-4aR,7S,9S-trihydroxy-2R-methyl-6S,8R-bis(methylamino)-4H-pyrano[3-b][1,4]benzodioxin-4-one, dihydrochloride pentahydrate | ||
| Canonical SMILES | [H][C@@]12[C@@](C(C[C@@H](C)O2)=O)(O)O[C@]3([H])[C@@H](NC)[C@@H](O)[C@@H](NC)[C@H](O)[C@@]3([H])O1.Cl.Cl.O.O.O.O.O | ||
| 分子式 | C14H24N2O7 • 2HCl [5H2O] | 分子量 | 495.4 |
| 溶解度 | ≥ 49.5mg/mL in H20, ≥ 92 mg/mL in DMSO, ≥ 3.61 mg/mL in EtOH with ultrasonic and warming | 储存条件 | Store at 2-8°C,argon filling away from moisture |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0186 mL | 10.0929 mL | 20.1857 mL |
| 5 mM | 0.4037 mL | 2.0186 mL | 4.0371 mL |
| 10 mM | 0.2019 mL | 1.0093 mL | 2.0186 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。