Sedanolide
(Synonyms: 新蛇床内酯) 目录号 : GC15216
Sedanolide是一种苯酞类化合物，作为高效的hMAO-B抑制剂，IC50值为0.124±0.033µM。
Cas No.:6415-59-4
Sample solution is provided at 25 µL, 10mM.
Sedanolide, one type of phthalein, is a potent hMAO-B inhibitor with an IC50 value of 0.124±0.033µM [1]. Sedanolide has COX-I and COX-II inhibitory activities and topoisomerase-I and topoisomerase-II inhibitory activities[2]. Sedanolide has been widely used in a variety of cancer cell models to inhibit cell growth and has inhibitory effects on a variety of bacteria and fungi[3].
In vitro, Sedanolide treatment at 500μM for 24 hours induced autophagy in J5 cells, which inhibited the PI3K/Akt/mTOR pathway[4]. Treatment with 500μM Sedanolide for 24h resulted in a significant increase in DNA strand breaks in HepG2 cells[5]. Treatment with 100μM Sedanolide for 24h protected luciferase-expressing HepG2 cells from H2O2-induced cell death and reduced intracellular ROS levels[6].
In vivo, Sedanolide treatment via daily administration at a dose of 20mg/kg/day for 14 consecutive days protected mice from dextran sodium sulfate (DSS)-induced colitis, alleviated intestinal inflammation, and enhanced intestinal barrier function[7]. Oral administration of Sedanolide (10mg/kg/day) for 14 consecutive days can activate the Nrf2 pathway and alleviate liver damage in mice treated with Acetaminophen (APAP)[8].
References:
[1] Fan Y, Wang J, Jian J, et al. High-throughput discovery of highly selective reversible hMAO-B inhibitors based on at-line nanofractionation[J]. Acta Pharmaceutica Sinica B, 2024, 14(4): 1772-1786.
[2] Momin R A, Nair M G. Antioxidant, cyclooxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. seeds[J]. Phytomedicine, 2002, 9(4): 312-318.
[3] Xie Q, Zhang X, Zhao S, et al. Sedanolide: A review on its chemical compounds, mechanisms and functions[J]. Fitoterapia, 2025: 106732.
[4] Hsieh S L, Chen C T, Wang J J, et al. Sedanolide induces autophagy through the PI3K, p53 and NF-κB signaling pathways in human liver cancer cells[J]. International journal of oncology, 2015, 47(6): 2240-2246.
[5] Woods J A, Jewell C, O'Brien N M. Sedanolide, a natural phthalide from celery seed oil: effect on hydrogen peroxide and tert-butyl hydroperoxide-induced toxicity in HepG2 and CaCo-2 human cell lines[J]. In Vitro & Molecular Toxicology: A Journal of Basic and Applied Research, 2001, 14(3): 233-240.
[6] Tabei Y, Abe H, Suzuki S, et al. Sedanolide activates KEAP1–NRF2 pathway and ameliorates hydrogen peroxide-induced apoptotic cell death[J]. International journal of molecular sciences, 2023, 24(22): 16532.
[7] Li S, Zhuge A, Chen H, et al. Sedanolide alleviates DSS-induced colitis by modulating the intestinal FXR-SMPD3 pathway in mice[J]. Journal of Advanced Research, 2025, 69: 413-426.
[8] Li S, Zhuge A, Xia J, et al. Bifidobacterium longum R0175 protects mice against APAP-induced liver injury by modulating the Nrf2 pathway[J]. Free Radical Biology and Medicine, 2023, 203: 11-23.
Sedanolide是一种苯酞类化合物，作为高效的hMAO-B抑制剂，IC50值为0.124±0.033µM[1]。Sedanolide兼具对COX-I和COX-II与拓扑异构酶-I和拓扑异构酶-II的抑制活性[2]。Sedanolide已在多种癌细胞模型中广泛应用于抑制细胞生长，并对多种细菌和真菌表现出抑制作用[3]。
在体外，使用500μM的Sedanolide处理J5细胞24小时可通过抑制PI3K/Akt/mTOR通路诱导细胞自噬[4]。500μM的Sedanolide处理HepG2细胞24小时可显著引起DNA链断裂[5]。100μM的Sedanolide处理表达荧光素酶的HepG2细胞24小时，能保护细胞免受过氧化氢诱导的死亡，并降低细胞内活性氧水平[6]。
在体内，连续14天每日口服20mg/kg/day剂量的Sedanolide，可保护小鼠免受葡聚糖硫酸钠(DSS)诱导的结肠炎损伤，减轻肠道炎症并增强肠道屏障功能[7]。连续14天每日口服10mg/kg/day剂量的Sedanolide，能激活Nrf2通路并缓解对乙酰氨基酚(APAP)引起的小鼠肝损伤[8]。
| Cell experiment [1]: | |
Cell lines | Luciferase-expressing HepG2 cells |
Preparation Method | Luciferase-expressing HepG2 cells were cultured at 37°C and 5% CO2 in a humidified environment in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum (FBS), 1% minimal essential medium nonessential amino acids (MEM), and 1mM sodium pyruvate. HepG2 cells expressing luciferase were seeded in 12-well plates at a density of 3.6×105 cells/well. After one day of incubation, cells were pretreated with 100µM Sedanolide for 24h and then exposed to hydrogen peroxide for an additional 24h before analysis of hydrogen peroxide-induced cell death. |
Reaction Conditions | 100µM; 24h |
Applications | Sedanolide pretreatment significantly inhibited hydrogen peroxide-induced cell death in Luciferase-expressing HepG2 cells. |
| Animal experiment [2]: | |
Animal models | Male C57BL/6J mice |
Preparation Method | Six-week-old male C57BL/6J mice were raised in an SPF environment and provided with adequate water and food. The mice were exposed to a 12-hour light/12-hour dark cycle and maintained at a temperature of 23±2°C. They were randomly divided into the NC group and the Sedanolide group. The mice were orally gavaged with Sedanolide (20mg/kg) or the vehicle every day for 14 consecutive days, starting from the 7th day to the 14th day. In the Sedanolide group, 2% DSS was given to the mice from the 7th day to the 14th day. On the 15th day, water was substituted for 2% DSS, and the mice were anesthetized and sacrificed on the 16th day. The colon tissues were collected for analysis. |
Dosage form | 20mg/kg/day for 14 days; p.o. |
Applications | Sedanolide treatment improved colitis symptoms, inflammation levels, and intestinal barrier function in mice. |
References: | |
| Cas No. | 6415-59-4 | SDF | |
| 别名 | 新蛇床内酯 | ||
| 化学名 | 3-butyl-3a,4,5,6-tetrahydro-1(3H)-isobenzofuranone | ||
| Canonical SMILES | O=C1OC(CCCC)C2CCCC=C21 | ||
| 分子式 | C12H18O2 | 分子量 | 194.3 |
| 溶解度 | ≥ 6.65mg/mL in DMSO | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.1467 mL | 25.7334 mL | 51.4668 mL |
| 5 mM | 1.0293 mL | 5.1467 mL | 10.2934 mL |
| 10 mM | 514.7 μL | 2.5733 mL | 5.1467 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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