RSPO1 Human
目录号 : GP24448R-Spondin-1 人类重组体
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | CHO cells. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | R-spondin-1; Cristin-3; mCristin-3; Roof plate-specific spondin-1; Rspo1; RSPO; R-spondin 1; R-spondin; Rspondin; CRISTIN3. | ||
| Amino Acid Sequence | RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA. | ||
| Solubility | It is recommended to reconstitute the lyophilized RSPO1in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized RSPO1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution RSPO1 should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50, as calculated by the Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a is less than 50ng/ml corresponding to a specific activity which is >2.0 x 10^4 units/mg. | ||
| Formulation | The protein was lyophilized from a sterile (0.2µm) filtered solution containing PBS. | ||
R-Spondin-1 (Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Rspo1 induces the onset of crypt cell proliferation and increases intestinal epithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway.
The ED50, as calculated by the Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a is less than 50ng/ml corresponding to a specific activity which is >2.0 x 10^4 units/mg.
Lyophilized RSPO1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution RSPO1 should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.