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Rhod-5N (potassium salt)

目录号 : GC44829

A low affinity fluorescent calcium probe

Rhod-5N (potassium salt) Chemical Structure

Cas No.:888229-31-0

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1mg
¥4,779.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

Rhod-5N is a low affinity fluorescent calcium probe (Kd = 320 µM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-5N has excitation/emission maxima of approximately 551 and 576 nm, respectively

Chemical Properties

Cas No. 888229-31-0 SDF
Canonical SMILES CN(C)C(C=C1)=CC2=C1C(C3=CC=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC([N+]([O-])=O)=C4)=C3)=C(C=C/5)C(O2)=CC5=[N+](C)\C.[K+].[K+].[K+]
分子式 C39H36N5O13•3K 分子量 900
溶解度 Water: Soluble 储存条件 Store at -20°C
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溶解性数据

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1 mM 1.1111 mL 5.5556 mL 11.1111 mL
5 mM 0.2222 mL 1.1111 mL 2.2222 mL
10 mM 0.1111 mL 0.5556 mL 1.1111 mL
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Research Update

Micro flow photochemical synthesis of Ca-sensitive fluorescent sensor particles

Eng Life Sci 2021 Jun 4;21(8-9):518-526.PMID:34584516DOI:PMC8456324

Fluorescence probes have widely been used for detecting and imaging Ca2+-enriched parts of cells but more rarely for quantitative determination of concentrations. In this study we show how this can be achieved by a novel approach using hydrogel particles. In a microfluidic co-flow arrangement spherical droplets were generated from an aqueous solution of acrylamide, N,N'-methylenebisacrylamide crosslinker and photoinitiator and subsequently photo-cured in situ yielding gel particles in a sub millimeter range. These particles were separated, dried under reduced pressure and re-swollen in water containing Rhod-5N tri potassium salt as calcium ion selective fluorescence probe. After that the particles were dried again and stored for further investigations. Upon exposure of dried particles to calcium chloride solutions they swell and take up Ca2+-ions forming a strong fluorescing complex with Rhod-5N. Thus, fluorescence intensity increases with calcium ion concentration. Up to ca. 0.50 mM the enhancement effect is strong and then becomes considerably weaker. The intensity-concentration-dependence is well described by an equation derived from the equilibrium of the formation of a 1:1 Ca2+:Rhod-5N complex. The particles allow for a fast optical determination of Ca2+-concentrations up to 0.50 mM in analyte volumes down to below 10 μL.