DNA/RNA Synthesis(DNA/RNA合成)
RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.
Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.
First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.
Products for DNA/RNA Synthesis
- Cat.No. 产品名称 Information
- GC18186 L67 A DNA ligase inhibitor
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GC15168
Urolithin A
A metabolite of ellagic acid with diverse biological activities
- GC15294 DPQ An inhibitor of poly(ADP-ribose) polymerases
- GC12266 Didox Antioxidant and ribonucleotide reductase inhibitor
- GC16949 Guanine A purine base
- GC12634 Deoxyguanosine 5-triphosphate A nucleoside triphosphate
- GC13399 Trimidox A ribonucleotide reductase inhibitor
- GC13510 3-AP A ribonucleotide reductase inhibitor
- GC10834 Isoxanthopterin An intermediate in the pteridine pathway
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GC15815
Thymidine
A pyrimidine nucleoside
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GC10867
(+)-Aphidicolin
Aphidicolin ((+)-Aphidicolin), a reversible inhibitor of eukaryotic nuclear DNA replication, can block the cell cycle at the pre-S phase[1].
- GC14808 Saccharin 1-methylimidazole (SMI) A chemical activator of DNA and RNA synthesis
- GC16879 Tubercidin A nucleoside analog with diverse biological activities
- GC14107 Oxolinic acid An inhibitor of bacterial DNA gyrase
- GC17232 P005672 hydrochloride A tetracycline antibiotic
- GC11654 Enocitabine A prodrug form of cytarabine
- GC12193 6-Thio-dG A purine nucleoside analog
- GC10620 E3330 An APE1/Ref-1 redox inhibitor
- GC17601 TH287 A selective MTH1 inhibitor
- GC12716 TH588 A selective MTH1 inhibitor
- GC15190 BMH-21 An RNA polymerase I inhibitor
- GC12106 SCR7 A DNA ligase IV inhibitor
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GC18094
Blasticidin S HCl
Blasticidin S HCl是一种从灰色链霉菌中分离的核苷类抗生素
- GC15238 Mupirocin An antibiotic
- GC12786 ML216 An inhibitor of the DNA repair enzyme Bloom helicase
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GC12777
8-Chloroadenosine
A nucleoside analog and an active metabolite of 8-chloro-cAMP
- GC12131 L189 A DNA ligase inhibitor
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GC11559
Anisomycin
An antibiotic that inhibits protein and DNA synthesis
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GC16384
Puromycin dihydrochloride
普罗霉素二盐酸盐是由革兰氏阳性放线菌链霉菌通过一系列酶反应产生的。
- GC13002 Thiostrepton An inhibitor of FoxM1
- GC14851 Vidofludimus An immunosuppressive drug that inhibits DHODH
- GC14066 Procaine An Analytical Reference Standard
- GC16843 Hydroxyurea An antineoplastic agent
- GC13742 Uridine A pyrimidine nucleoside
- GC11825 Adenine HCl Adenine HCl (6-Aminopurine hydrochloride) 是一种嘌呤,是 DNA 核酸中的四种核碱基之一。盐酸腺嘌呤充当 DNA 和 RNA 的化学成分。腺嘌呤 HCl 在涉及细胞呼吸、ATP 和辅因子(NAD 和 FAD)的形式以及蛋白质合成的生物化学中也发挥着重要作用。
- GC17750 Raltitrexed An inhibitor of thymidylate synthase
- GC11793 Procarbazine HCl A DNA methylating agent
- GC17278 Adenine sulfate A purine base
- GC15786 Nedaplatin An anticancer agent
- GC14885 Foscarnet Sodium Foscarnet sodium (Trisodium phosphonoformate) 是一种病毒 DNA 聚合酶活性抑制剂,可导致病毒复制的可逆抑制。
- GC15033 Azathioprine An immunosuppressive purine analog
- GC12423 Mycophenolate mofetil hydrochloride A prodrug form of mycophenolic acid
- GC13432 Adenine 腺嘌呤(6-氨基嘌呤)是一种嘌呤,是DNA核酸中的四种核碱基之一。腺嘌呤是DNA和RNA的化学成分。腺嘌呤还在涉及细胞呼吸、ATP 和辅因子(NAD 和 FAD)的形式以及蛋白质合成的生物化学中发挥重要作用。
- GC12350 T 705 An antiviral agent
- GC14961 Cytarabine hydrochloride A nucleoside analog and prodrug form of ara-CTP
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GC12828
Daunorubicin
Daunorubicin (Daunomycin) 是一种拓扑异构酶 II 抑制剂,具有有效的抗肿瘤活性。
- GC10354 Daunorubicin HCl Antitumor antibiotic used in the treatment of acute myeloid leukemias
- GC17897 Triapine 核糖核苷酸还原酶抑制剂
- GC17231 CRT0044876 A cell-permeable inhibitor of Ape-1
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GC17716
Oxaliplatin
A DNA-crosslinking agent