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CRISPR/Cas9

CRISPR/Cas9 (Clustered Regularly-Interspaced Short Palindromic Repeats/Cas9) is a bacterial immune system that has been adapted for genome editing in mammalian cells. Cas9 is a programmable nuclease that generates double-stranded breaks (DSB) in DNA dictated by binding of a ~20 nucleotide recombinant “guide RNA” (gRNA) to the target site. DSB’s produced by Cas9 are most often repaired through the cell’s error-prone non-homologous end joining (NHEJ) pathway, resulting in small insertions or deletions (indels). The vast majority of indels shift the reading frame, introducing a premature stop codon or resulting in nonsense mediated decay of the mRNA-effectively “knocking out” a gene.

The CRISPR/Cas9 system has substantially advanced efforts in specific gene editing and has been successfully applied to modify the episomal genomes of human and other organisms. The CRISPR/Cas9 system utilizes a prokaryotic RNA-guided programmable nuclease that can make a double-strand DNA break (DSB) at a specific site under the guidance of a leading RNA. This DSB process depends on the co-expression of two basic components: a guide RNA (gRNA) and Cas9 nuclease. Making a specific DSB can trigger DNA repair via either error-prone non-homologous end joining (NHEJ) or homology-directed repair (HDR). In the presence of the CRISPR/Cas9 system, the NHEJ inhibitor SCR7 is proven to increase the efficiency of Cas9-mediated HDR by at least by 7-fold in mammalian cells. Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells.

Products for  CRISPR/Cas9

  1. Cat.No. 产品名称 Information
  2. GC68804 BRD7586 BRD7586 是 SpCas9 的细胞渗透性小分子抑制剂。BRD7586 是已知最小的抗 CRISPR 抑制剂。
  3. GC64111 BRD0539 BRD0539 is a potent inhibitor of Streptococcus pyogenes Cas9 (SpCas9) with IC50 of 22 μM in an in vitro DNA cleavage assay.
  4. GC16265 RS-1 A RAD51 activator
  5. GC12106 SCR7 A DNA ligase IV inhibitor
  6. GC15743 L-755,507 A selective β3-adrenergic receptor partial agonist
  7. GC17683 Brefeldin A

    布雷菲定A(BFA)是一种真菌大环内酯,能够有效、可逆地抑制细胞内囊泡形成和内质网(ER)与高尔基体之间的蛋白质运输。

  8. GC10637 Zidovudine A nucleoside reverse transcriptase inhibitor
  9. GC14075 Nocodazole

    A microtubule altering agent

  10. GC11251 NU7441 (KU-57788) A selective DNA-PK inhibitor

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