ML-7 hydrochloride
(Synonyms: 1-(5-碘萘-1-磺酰基)-1H-六氢-1,4-二氮杂卓盐酸盐,ML 7 hydrochloride) 目录号 : GC11411
ML-7 hydrochloride是一种选择性肌球蛋白轻链激酶（IC50 = 300nM）。
Cas No.:110448-33-4
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
ML-7 hydrochloride is a selective myosin light chain kinase (IC50 = 300nM) [1]. ML-7 hydrochloride competitively inhibits the ATP binding activity of myosin light chain kinase (MLCK), thereby inhibiting MLC phosphorylation and regulating cytoskeleton, contraction and migration behavior [2]. ML-7 hydrochloride is used in research on cell biology, oncology, vascular physiology [3-4].
In leukocyte, ML-7 hydrochloride (20μM; 24h) treatment induces lysosome-mediated cell death in neutrophils from patients with sepsis [5]. In Klebsiella pneumoniae, ML-7 hydrochloride (64μg/mL; 24h) treatment could enhance the killing activity of tigecycline [6]. In oeFOXP2 cells, ML-7 hydrochloride (40μM; 48h) administration markedly increased the proliferation of cell [7].
In Lewis lung carcinoma cell subcutaneous tumor mice model, ML-7 hydrochloride (0.25mg/kg; ip; 7d) pretreated tumors were significantly smaller than those pretreated with vehicle control [8]. In the mouse model treated with trimellitic anhydride, injection of ML-7 hydrochloride (1mg/kg; ip; 5d) significantly inhibited the exacerbation of scratching behavior induced by immobilization stress [9].
References:
[1]. Simoes RL, Fierro IM. Involvement of the Rho-kinase/myosin light chain kinase pathway on human monocyte chemotaxis induced by ATL-1, an aspirin-triggered lipoxin A4 synthetic analog. The Journal of Immunology. 2005 Aug 1; 175(3): 1843-1850.
[2]. Ran Q, Li A, Tan Y, et al. Action and therapeutic targets of myosin light chain kinase, an important cardiovascular signaling mechanism. Pharmacological Research. 2024 Jun 27: 107276.
[3]. Xiong Y, Wang C, Shi L, et al. Myosin light chain kinase: a potential target for treatment of inflammatory diseases. Frontiers in pharmacology. 2017 May 23; 8: 292.
[4]. Gu LZ, Hu WY, Antic N, et al. Inhibiting myosin light chain kinase retards the growth of mammary and prostate cancer cells. European journal of cancer. 2006 May 1; 42(7): 948-957.
[5]. Wu J, Barkat MQ, Su J, et al. Inhibition of non-muscular myosin light chain kinase accelerates the clearance of inflammatory cells by promoting the lysosome-mediated cell death. Biomedicine & Pharmacotherapy. 2024 Jan 1; 170: 115986.
[6]. Sun L, Sun L, Li X, et al. A novel tigecycline adjuvant ML-7 reverses the susceptibility of tigecycline-resistant Klebsiella pneumoniae. Frontiers in Cellular and Infection Microbiology. 2022 Jan 5; 11: 809542.
[7]. Chang S. Effect of forkhead box protein P2-mediated activation of myosin light-chain kinase on the invasion and migration of endometrial cancer cells. CytoJournal. 2025 May 14; 22: 54.
[8]. Kim YE, Gwak SH, Hong BJ, et al. Effects of ultra-high doserate FLASH irradiation on the tumor microenvironment in Lewis lung carcinoma: role of myosin light chain. International Journal of Radiation Oncology* Biology* Physics. 2021 Apr 1;109(5):1440-1453.
[9]. Cho DE, Hong JP, Kim Y, et al. Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis. Scientific reports. 2024 Mar 15; 14(1): 6263.
ML-7 hydrochloride是一种选择性肌球蛋白轻链激酶（IC50 = 300nM） [1]。ML-7 hydrochloride竞争性抑制肌球蛋白轻链激酶（MLCK）的ATP结合活性，从而抑制MLC磷酸化并调节细胞骨架、收缩和迁移行为 [2]。ML-7 hydrochloride用于细胞生物学、肿瘤学和血管生理学的研究 [3-4]。
在白细胞中，ML-7 hydrochloride（20μM；24h）处理可诱导脓毒症患者中性粒细胞发生溶酶体介导的细胞死亡 [5]。在肺炎克雷伯菌中，ML-7盐酸盐（64μg/mL；24h）处理可增强替加环素的杀灭活性 [6]。在oeFOXP2细胞中，给予ML-7 hydrochloride（40μM；48h）可显著增加细胞增殖 [7]。
在Lewis肺癌细胞皮下移植瘤小鼠模型中，ML-7 hydrochloride（0.25mg/kg；ip；7d）预处理的肿瘤显著小于用载体对照组预处理的肿瘤 [8]。在用偏苯三酸酐处理的小鼠模型中，注射ML-7 hydrochloride（1mg/kg；ip；5d）可显著抑制因束缚应激引起的抓挠行为的加剧 [9]。
| Cell experiment [1]: | |
Cell lines | Leukocyte |
Preparation Method | The white cell layer at the separating fluid boundary was collected and washed three times with PBS. Cell pellet was resuspended in RPM1640 medium and seeded in glass slides coated with polyL. Cell was collected at 0h or 24h for TUNEL or immunofluorescence staining after incubating with ML-7 hydrochloride (20μM) or vehicle. |
Reaction Conditions | 20μM; 24h |
Applications | ML-7 hydrochloride treatment induces lysosome-mediated cell death in neutrophils from patients with sepsis. |
| Animal experiment [2]: | |
Animal models | Lewis lung carcinoma cell subcutaneous tumor mice model |
Preparation Method | For in vivo experiments, Lewis lung carcinoma (LLC) cells were implanted subcutaneously in 6-week-old male C57BL/6 mice and irradiated when the tumor size reached approximately 150mm2. For ML-7 treatment, LLC tumor–bearing mice were injected with ML-7 (0.25mg/kg) or vehicle intraperitoneally every day for 7 days before 15 Gy IR. |
Dosage form | 0.25mg/kg; ip; 7d |
Applications | ML-7 hydrochloride pretreated tumors were significantly smaller than those pretreated with vehicle control. |
References: | |
| Cas No. | 110448-33-4 | SDF | |
| 别名 | 1-(5-碘萘-1-磺酰基)-1H-六氢-1,4-二氮杂卓盐酸盐,ML 7 hydrochloride | ||
| 化学名 | 1-((5-iodonaphthalen-1-yl)sulfonyl)-1,4-diazepane hydrochloride | ||
| Canonical SMILES | IC1=CC=CC2=C1C=CC=C2S(N3CCCNCC3)(=O)=O.Cl | ||
| 分子式 | C15H18ClIN2O2S | 分子量 | 452.74 |
| 溶解度 | ≥ 15.95 mg/mL in DMSO, ≥ 8.82 mg/mL in Water with ultrasonic and warming | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.2088 mL | 11.0439 mL | 22.0877 mL |
| 5 mM | 0.4418 mL | 2.2088 mL | 4.4175 mL |
| 10 mM | 0.2209 mL | 1.1044 mL | 2.2088 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet