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Protease Inhibitor Cocktail II (EDTA-Free, 200X in DMSO)

目录号 : GK10016

相当于 Sigma P1860,用于组织培养基以防止分泌蛋白的蛋白水解降解。

Protease Inhibitor Cocktail II (EDTA-Free, 200X in DMSO) Chemical Structure

规格 价格 库存 购买数量
1mL
¥224.00
现货
1mL x 20
¥3,200.00
现货
1mL x 50
¥7,200.00
现货
1mL x 100
¥10,800.00
现货

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Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

客户使用产品发表文献 2

产品文档

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实验参考方法

It is suggested to dilute the cocktail appropriately for specific cell lines. The test should begin with at least 200-fold dilution because concentrations of DMSO greater than 0.5% may be deleterious for cells. Since various cell lines will differ in their sensitivity, further dilutions may be needed for this cocktail. This cocktail remains effective for up to 48 hours in the medium. After 48 hours, the medium should be replaced with fresh medium containing the cocktail.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc

产品描述

Secreted protein production is halted and degradation is increased when proteins are extracted from media in vitro. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in tissue culture media to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates. This cocktail should be used as a supplement to tissue culture media to prevent the degradation of secreted proteins.

This protease inhibitor cocktail contains individual components, including Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for tissue culture media. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Ingredient   

Summary                CAS Number Targets    
Aprotinin  Inhibitor of bovine pancreatic trypsin 9087-70-1 Proteases|Serine Protease
Bestatin Aminopeptidase inhibitor 58970-76-6 Proteases|Aminopeptidase
E-64 Cysteine protease inhibitor,irriversible 66701-25-5 Proteases|Cathepsin
Leupeptin, Microbial Inhibitor of serine and cysteine proteases 103476-89-7 Proteases|Serine Protease
Pepstatin A Aspartic proteinases inhibitor 26305-03-3  Proteases|Other Proteases