Prostaglandin D2 serinol amide
(Synonyms: PGD2SA) 目录号 : GC44709
A stable analog of PGD2 2-glyceryl ester
Cas No.:851761-42-7
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2-Arachidonyl glycerol (2-AG) exhibits cannabinoid (CB) agonist activity at the CB1 receptor, is an important endogenous monoglyceride species, and is thus considered to be the natural ligand for the CB1 receptor. 2-AG can also be sequentially metabolized by COX-2 and specific PG synthases to form PG 2-glyceryl esters. In activated RAW 264.7 cells, PGD2 2-glyceryl ester is the primary product of 2-AG metabolism in the COX pathway. PGD2 serinol amide (PGD2-SA) is a stable analog of PGD2 2-glyceryl ester. Unlike PGD2 2-glyceryl ester and other fatty acyl 2-glyceryl esters, PGD2-SA will not isomerize to the less active primary (1-glyceryl) ester. The biological activity of PGD2-SA has not yet been determined.
| Cas No. | 851761-42-7 | SDF | |
| 别名 | PGD2SA | ||
| Canonical SMILES | O[C@@H](C1)[C@H](C/C=C\CCCC(N(C(CO)CO)[H])=O)[C@@H](/C=C/[C@@H](O)CCCCC)C1=O | ||
| 分子式 | C23H39NO6 | 分子量 | 425.6 |
| 溶解度 | DMF: 25 mg/ml,DMSO: 25 mg/ml,Ethanol: 25 mg/ml,PBS (pH 7.2): 5 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3496 mL | 11.7481 mL | 23.4962 mL |
| 5 mM | 0.4699 mL | 2.3496 mL | 4.6992 mL |
| 10 mM | 0.235 mL | 1.1748 mL | 2.3496 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Cyclooxygenation of the arachidonoyl side chain of 1-arachidonoylglycerol and related compounds block their ability to prevent anandamide and 2-oleoylglycerol metabolism by rat brain in vitro
Biochem Pharmacol 2005 Apr 15;69(8):1241-5.PMID:15794945DOI:10.1016/j.bcp.2005.01.016
In the present study, the abilities of cyclooxygenated derivatives of 1-arachidonoylglycerol and related compounds to prevent the metabolism of [3H]2-oleoylglycerol and [3H]anandamide by cytosolic and membrane fractions, respectively, have been investigated. For each compound, nine concentrations (range 0.2-100 microM) were tested. 1-Arachidonoylglycerol inhibited the hydrolysis of [3H]2-oleoylglycerol with a pI50 value of 5.17+/-0.04 (maximum attainable inhibition 88%). In contrast, the 1-glyceryl esters of prostaglandin D2, E2 and F2alpha were very weak inhibitors of this hydrolysis. Similarly, prostaglandin D2, prostaglandin D2 ethanolamide and Prostaglandin D2 serinol amide produced <20% inhibition of [3H]2-oleoylglycerol metabolism at any concentration tested, in contrast to previous data with arachidonic acid, anandamide and arachidonoyl serinol which are all able to inhibit metabolism of this substrate under the assay conditions used here. A similar pattern was seen for all the compounds with respect to the inhibition of [3H]anandamide hydrolysis by the membrane fractions. Thus, cyclooxygenation of the arachidonoyl side chain greatly reduces the ability of 1-arachidonoylglycerol and related compounds to inhibit the hydrolysis of [3H]2-oleoylglycerol and [3H]anandamide.