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PLA2G1B Human

目录号 : GP22096

Secreted Phospholipase A2-IB Human Recombinant

PLA2G1B Human Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥54,600.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Secreted Phospholipase A2-IB Human Recombinant is manufactured with N-terminal fusionf HisTag. PLA2G1B His-Tagged Fusion Protein is 16 kDa containing 126 amino acid residues of the human secreted phospholipase A2-IB and 16 additional amino acid residues - HisTag (underlined).

Product Data

Purity Greater than 95% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Phospholipase A2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase; Group IB phospholipase A2; PLA2; PLA2A; PPLA2; sPLA2-IB;MGC119834; MGC119835; PLA2G1B.
Amino Acid Sequence MRGSHHHHHH GMASHMAVWQFRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYSYSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS.
Solubility Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ж╠g/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability Store lyophilized protein at -20°C . Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C .
Formulation Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Introduction

Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentrationdependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.

Stability

Store lyophilized protein at -20°C . Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C .