Piliformic Acid
目录号 : GC48919A fungal metabolite
Cas No.:98985-76-3
Sample solution is provided at 25 µL, 10mM.
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Piliformic acid is a fungal metabolite that has been found in N. pseudotrichia and has diverse biological activities.1,2 It is cytotoxic to BC-1 human breast cancer cells (IC50 = 5 µg/ml).2 Piloformic acid is active against L. braziliensis amastigotes (IC50 = 78.5 µM). It is also active against the plant pathogenic fungi C. gloeosporioides (MIC = 292 µM).1
1.Elias, L.M., Fortkamp, D., Sartori, S.B., et al.The potential of compounds isolated from Xylaria spp. as antifungal agents against anthracnoseBraz. J. Microbiol.49(4)840-847(2018) 2.Cota, B.B., Tunes, L.G., Maia, D.N.B., et al.Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood)Mem. Inst. Oswaldo Cruz113(2)102-110(2018)
Cas No. | 98985-76-3 | SDF | |
Canonical SMILES | OC(C(C)/C(C(O)=O)=C\CCCCC)=O | ||
分子式 | C11H18O4 | 分子量 | 214.3 |
溶解度 | 储存条件 | -20°C | |
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1 mg | 5 mg | 10 mg | |
1 mM | 4.6664 mL | 23.3318 mL | 46.6636 mL |
5 mM | 0.9333 mL | 4.6664 mL | 9.3327 mL |
10 mM | 0.4666 mL | 2.3332 mL | 4.6664 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Leishmanicidal compounds of Nectria pseudotrichia, an endophytic fungus isolated from the plant Caesalpinia echinata (Brazilwood)
Mem Inst Oswaldo Cruz 2018 Feb;113(2):102-110.PMID:29236928DOI:10.1590/0074-02760170217.
BACKGROUND In a screen of extracts from plants and fungi to detect antileishmanial activity, we found that the ethyl acetate extract of the fungus Nectria pseudotrichia, isolated from the tree Caesalpinia echinata (Brazilwood), is a promising source of bioactive compounds. OBJECTIVES The aims of this study were to isolate and determine the chemical structures of the compounds responsible for the antileishmanial activity of the organic extract from N. pseudotrichia. METHODS Compounds were isolated by chromatographic fractionation using semi-preparative high-performance liquid chromatography, and their chemical structures were determined by analytical and spectral data and by comparison with published data. The antileishmanial activity of the isolated compounds was evaluated in intracellular amastigote forms of Leishmania (Viannia) braziliensis expressing firefly luciferase as reporter gene, and cytotoxicity was determined in Vero and THP-1 mammalian cell lines by MTT assay. FINDINGS Fractionation of the extract yielded seven compounds: 10-acetyl trichoderonic acid A (1), 6'-acetoxy-piliformic acid (2), 5',6'-dehydropiliformic acid (3), Piliformic Acid (4), hydroheptelidic acid (5), xylaric acid D (6), and cytochalasin D (7). Compounds 1, 2 and 3 are reported here for the first time. Compounds 1, 2, and 5 were more active, with IC50 values of 21.4, 28.3, and 24.8 µM, respectively, and showed low toxicity to Vero and THP-1 cells. MAIN CONCLUSIONS N. pseudotrichia produces secondary metabolites that are more toxic to intracellular amastigote forms of L. (V.) braziliensis than to mammalian cells.
The potential of compounds isolated from Xylaria spp. as antifungal agents against anthracnose
Braz J Microbiol 2018 Oct-Dec;49(4):840-847.PMID:29631892DOI:10.1016/j.bjm.2018.03.003.
Anthracnose is a crop disease usually caused by fungi in the genus Colletotrichum or Gloeosporium. These are considered one of the main pathogens, causing significant economic losses, such as in peppers and guarana. The current forms of control include the use of resistant cultivars, sanitary pruning and fungicides. However, even with the use of some methods of controlling these cultures, the crops are not free of anthracnose. Additionally, excessive application of fungicides increases the resistance of pathogens to agrochemicals and cause harm to human health and the environment. In order to find natural antifungal agents against guarana anthracnose, endophytic fungi were isolated from Amazon guarana. The compounds Piliformic Acid and cytochalasin D were isolated by chromatographic techniques from two Xylaria spp., guided by assays with Colletotrichum gloeosporioides. The isolated compounds were identified by spectrometric techniques, as NMR and mass spectrometry. This is the first report that Piliformic Acid and cytochalasin D have antifungal activity against C. gloeosporioides with MIC 2.92 and 2.46μmolmL-1 respectively. Captan and difenoconazole were included as positive controls (MIC 16.63 and 0.02μmolmL-1, respectively). Thus, Xylaria species presented a biotechnological potential and production of different active compounds which might be promising against anthracnose disease.