Phosphocreatine disodium salt
(Synonyms: 磷酸肌酸钠,Disodium creatine phosphate) 目录号 : GC13228
Phosphocreatine disodium salt是肌肉和大脑等高能量波动需求组织中的重要能量储备物质。
Cas No.:922-32-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Phosphocreatine disodium salt is a crucial energy store in tissues with high, fluctuating energy demands like muscle and brain [1]. Phosphocreatine disodium salt interacts with phospholipids to influence membrane properties and exert membrane protection [2]. Phosphocreatine disodium salt is an effective and safe cardioprotective agent, which has been widely used in different animal models to protect the heart from intraoperative injury and improve cardiac function[3].
In vitro, Phosphocreatine disodium salt pretreatment at 20mM for 2 hours significantly attenuated methylglyoxal (MGO)-induced PC12 cell damage and inhibited MGO-induced apoptosis[4]. Pretreatment of NRK-52E cells with 40mM Phosphocreatine disodium salt for 4 hours significantly restored the viability of MGO-injured NRK-52E cells, resulting in a significant decrease in MDA levels, a significant increase in GSH and SOD levels, and inhibition of intracellular ROS production[5]. Pretreatment of H9C2 cells with 20mM Phosphocreatine disodium salt for 2h attenuated the cytotoxicity of recovered MGO, significantly increased Bcl-2 expression, decreased Bax expression, and improved mitochondrial respiration[6].
In vivo, a single dose of Phosphocreatine disodium salt (400mg/kg) administered intravenously 30min before cerebral ischemia-reperfusion (IR) can protect neurological function, reduce infarct volume, and brain cell apoptosis in rats 72 hours after focal cerebral IR[7]. Daily oral administration of Phosphocreatine disodium salt in drinking water (78mM) for one week reduced the severity of dextran sulfate sodium (DSS)-induced colitis in mice[8].
References:
[1] Guimarães-Ferreira L. Role of the phosphocreatine system on energetic homeostasis in skeletal and cardiac muscles[J]. Einstein (Sao Paulo), 2014, 12: 126-131.
[2] Tokarska-Schlattner M, Epand R F, Meiler F, et al. Phosphocreatine interacts with phospholipids, affects membrane properties and exerts membrane-protective effects[J]. 2012.
[3] Xi H, Zhang A, Han G, et al. Pharmacokinetics and hemorheology of phosphocreatine and creatine in rabbits: A directly comparative study between parent drug and active metabolite[J]. European Journal of Pharmaceutical Sciences, 2019, 138: 105033.
[4] Li H, Tang Z, Chu P, et al. Neuroprotective effect of phosphocreatine on oxidative stress and mitochondrial dysfunction induced apoptosis in vitro and in vivo: Involvement of dual PI3K/Akt and Nrf2/HO-1 pathways[J]. Free Radical Biology and Medicine, 2018, 120: 228-238.
[5] Shopit A, Niu M, Wang H, et al. Protection of diabetes-induced kidney injury by phosphocreatine via the regulation of ERK/Nrf2/HO-1 signaling pathway[J]. Life Sciences, 2020, 242: 117248.
[6] Qaed E, Wang J, Almoiliqy M, et al. Phosphocreatine improves cardiac dysfunction by normalizing mitochondrial respiratory function through JAK2/STAT3 signaling pathway in vivo and in vitro[J]. Oxidative medicine and cellular longevity, 2019, 2019(1): 6521218.
[7] Li T, Wang N, Zhao M. Neuroprotective effect of phosphocreatine on focal cerebral ischemia‐reperfusion injury[J]. BioMed Research International, 2012, 2012(1): 168756.
[8] Bhagavatula G, Worledge C S, Schaepe C, et al. Phosphocreatine rescues intestinal epithelial metabolic dysfunction related to creatine kinase loss and is protective in murine colitis[J]. Cellular and Molecular Gastroenterology and Hepatology, 2025: 101557.
Phosphocreatine disodium salt是肌肉和大脑等高能量波动需求组织中的重要能量储备物质[1]。Phosphocreatine disodium salt通过与磷脂相互作用影响膜特性并发挥膜保护作用[2]。Phosphocreatine disodium salt作为一种有效且安全的心脏保护剂，已广泛应用于不同动物模型中预防术中心脏损伤并改善心功能[3]。
在体外，20mM的Phosphocreatine disodium salt预处理PC12细胞2小时可显著减轻甲基乙二醛（MGO）诱导的细胞损伤并抑制凋亡[4]。40mM的Phosphocreatine disodium salt预处理NRK-52E细胞4小时能恢复MGO损伤的细胞活力，显著降低MDA水平、提高GSH和SOD水平并抑制细胞内ROS产生[5]。20mM的Phosphocreatine disodium salt预处理H9C2细胞2小时可缓解MGO的细胞毒性，显著增加Bcl-2表达、降低Bax表达并改善线粒体呼吸功能[6]。
在体内，大鼠脑缺血再灌注（IR）前30分钟单次静脉注射Phosphocreatine disodium salt（400mg/kg）可在IR后72小时保护神经功能、减少梗死体积和脑细胞凋亡[7]。小鼠通过饮用水摄入Phosphocreatine disodium salt（78mM；持续一周）可减轻葡聚糖硫酸钠（DSS）诱导的结肠炎严重程度[8]。
| Cell experiment [1]: | |
Cell lines | PC12 cells |
Preparation Method | PC12 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, 100U/mL penicillin, and 100U/mL streptomycin, and cultured in a 5% CO2 saturated humidified incubator at 37℃. PC-12 cells were seeded in 96-well plates at a density of 5×104cells/ml/well and cultured for 24 hours. Cell viability was analyzed by pretreatment with various concentrations of Phosphocreatine disodium salt (5, 10, and 2mM) for 2h followed by stimulation with methylglyoxal (MGO) (0.8mM) for 24h. To examine cell morphology, cells were seeded in 6-well plates and pretreated with Phosphocreatine disodium salt for 2h, respectively, followed by stimulation with MGO (0.8mM) for 24h. Images were taken using an inverted microscope. |
Reaction Conditions | 5, 10, and 2mM; 2h |
Applications | Phosphocreatine disodium salt treatment significantly increased the cell viability and restored the morphological changes following MGO-induced injury. |
| Animal experiment [2]: | |
Animal models | C57Bl/6 mice |
Preparation Method | Acute colitis was induced in C57Bl/6 mice using drinking water containing 2.5% DSS for a total of 5 days and then discontinued 2 days before sacrifice. Mice in the Phosphocreatine disodium salt-treated group drank drinking water containing 78mM Phosphocreatine disodium salt from 2 days before DSS initiation until death. If non-DSS control mice were included, mice were given either untreated drinking water or water containing 78mM Phosphocreatine disodium salt for the duration of the experiment. Colon length (from cecum to anus) was measured. The colon was skinned, washed, and colonic tissue fragments were collected and fixed in formalin for histological analysis. |
Dosage form | 5mM in drinking water/day for 1 week; p.o. |
Applications | Phosphocreatine disodium salt treatment significantly attenuated the severity of DSS-induced colitis in mice. |
References: | |
| Cas No. | 922-32-7 | SDF | |
| 别名 | 磷酸肌酸钠,Disodium creatine phosphate | ||
| 化学名 | sodium (E)-(amino((carboxymethyl)(methyl)amino)methylene)phosphoramidate | ||
| Canonical SMILES | OC(CN(C)/C(N)=N/P([O-])([O-])=O)=O.[Na+].[Na+] | ||
| 分子式 | C4H8N3Na2O5P | 分子量 | 255.08 |
| 溶解度 | ≥ 54.6 mg/mL in Water | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.9203 mL | 19.6017 mL | 39.2034 mL |
| 5 mM | 784.1 μL | 3.9203 mL | 7.8407 mL |
| 10 mM | 392 μL | 1.9602 mL | 3.9203 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet