P4HB Mouse
目录号 : GP22042Prolyl 4-Hydroxylase Beta Mouse Recombinant
Sample solution is provided at 25 µL, 10mM.
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P4HB produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 498 amino acids (20-509a.a.) andhaving a molecular mass of 56.1kDa.(Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).P4HB is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Sf9,Baculovirus cells. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Protein disulfide-isomerase; PDI; Cellular thyroidhormone-binding protein; Endoplasmic reticulum resident protein 59; ER protein59; ERp59; Prolyl 4-hydroxylase subunit beta; p55. | ||
| Amino Acid Sequence | DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAAAKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKN GDTASPKEYT AGREADDIVN WLKKRTGPAATTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQLDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPKIFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRLITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANFEEVAFDEKKN VFVEFYAPWCGHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDGAGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | P4HB protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. | ||
P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P4HB is a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.