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Neurotensin (trifluoroacetate salt) Sale

目录号 : GC44387

A neuropeptide with diverse biological activities

Neurotensin (trifluoroacetate salt) Chemical Structure

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1mg
¥1,284.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

Neurotensin is a neuropeptide that is distributed throughout the CNS and in enteroendocrine cells of the small intestine that has diverse biological activities. In vivo, neurotensin (10-50 μg per hour, i.c.v.) induces hypothermia in rats. Neurotensin signaling is increased and induces analgesia in wild-type mice, and neurotensin knockout mice are defective in basal nociceptive and stress-induced analgesic responses in a cold water swim stress test. It acts as an indirect dopamine antagonist that inhibits dopamine-induced effects at pre- and postsynaptic dopamine receptors. Neurotensin inhibits small bowel motility and secretion of gastric acid, stimulates pancreatic and biliary secretion, increases fatty acid absorption, and stimulates growth of the stomach, colon, pancreas, and small bowel in rats. It also increases growth of breast, pancreatic, and hepatocellular carcinoma cells via induction of IL-8 secretion and activation of epithelial mesenchymal transition (EMT) in vitro and in vivo.

Chemical Properties

Cas No. SDF
分子式 C78H121N21O20•XCF3COOH 分子量 1672.9
溶解度 Water: 1 mg/ml 储存条件 Store at -20°C
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溶解性数据

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1 mM 0.5978 mL 2.9888 mL 5.9776 mL
5 mM 0.1196 mL 0.5978 mL 1.1955 mL
10 mM 0.0598 mL 0.2989 mL 0.5978 mL
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Research Update

Peptide characterization with a sulfoethyl aspartamide column

J Chromatogr 1988 Jun 29;443:63-71.PMID:2844842DOI:10.1016/s0021-9673(00)94783-6.

A strong cation-exchange (SCX) high-performance liquid chromatography column (sulfoethyl aspartamide, 200 x 4.6 mm) was used to analyze more than 50 peptides, ranging in length from 5 to 20 residues. These data show that the elution positions of the peptides increase monotonically with the number of positively charged residues. [A 60-min linear gradient of 0 to 100% eluent B at 1 ml/min was used, where eluent A is 5 mM phosphate (pH 3.0)-acetonitrile (75:25) and eluent B is eluent A + 0.5 M sodium chloride.] A comparison of SCX with a standard C18 reversed-phase (RP) column [60-min linear gradient of 0 to 60% B at 1 ml/min, where eluent A is 0.1% trifluoroacetic acid (TFA), and eluent B is 0.095% TFA-acetonitrile (10:90)] further demonstrates the utility of SCX in peptide characterization. SCX separated an (Arg)3-containing peptide from the Arg-deleted peptide while RP could not. In addition, SCX and RP resolved the methionine oxidation products of ACTH (4-10) (RP: Met [O] less than Met [O2] less than Met; SCX; Met [O] less than Met less than Met [O2]), suggesting a mixed-mode mechanism for the ion-exchange system. Finally, SCX separated the sulfated and non-sulfated forms of cholecystokinin (26-33) and Leu-enkephalin as well as the N-terminal acetylated forms of Neurotensin (8-13) and angiotensinogen (1-14) from the respective unmodified peptides.

High-performance liquid chromatography of neuropeptides using radially compressed polythene cartridges

J Chromatogr 1984 Mar 9;306:99-108.PMID:6325486DOI:10.1016/s0378-4347(00)80873-7.

This study was designed to assess practically the suitability of different C18 reversed-phase radially compressed polythene cartridges (Radial-Pak, Waters Assoc.) in two types of radial-compression systems, for the separation and analysis of various neuropeptides at both high (less than 5 micrograms) and low (greater than 100 pg) levels in biological extracts and to compare them with well established techniques using stainless-steel columns. A solvent system fully compatible with both radially compressed and steel columns is described. The completely volatile mobile phase (acetonitrile gradient containing trifluoroacetic acid) allows ultraviolet detection below 215 nm, gives good resolution and is readily compatible with the further radioimmunoassay and bioassay of collected fractions. The efficiency of radially compressed 5 and 10 microns "capped" and "non-capped" C18 silica supports and slurry-packed steel columns has been assessed by: (1) separation and recovery of a complex standard mixture of neuropeptides; (2) separation and subsequent identification of degradation products formed during the incubation of Neurotensin with rat cortical synaptosomes; (3) analysis of alpha-melanotropin and corticotropin-(18-39) in tissue culture media containing varying amounts of foetal calf serum; and (4) characterization of corticotropin-like immunoreactivity in human cerebrospinal fluid. The Z-module fitted with the capped 10-microns irregular C18 silica cartridge gave better resolution than with the mu Bondapak steel column but the selective retention was similar. The back-pressures in the Z-module are much reduced (approximately 13 bar at 1 ml/min); therefore, flow-rates may be increased and analysis times greatly reduced. In order to obtain good resolution with the RCM-100 module which uses a non-capped stationary phase, a salt must be added (e.g. 15 mM sodium chloride) to the mobile phase to reduce polar interactions between the peptide and the free silanol groups on the stationary phase. This makes the solvent non-volatile and therefore less useful.