N-3-hydroxydecanoyl-L-Homoserine lactone
(Synonyms: OH-C10-HSL, 3OH-C10-HSL, 3OH-C10-L-HSL) 目录号 : GC40660A bacterial quorum-sensing signaling molecule
Cas No.:192883-12-8
Sample solution is provided at 25 µL, 10mM.
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Quorum sensing is a regulatory system used by bacteria for controlling gene expression in response to increasing cell density. Different quorum sensing molecules are produced at different times in bacterial population growth and have distinct cellular effects mediated through changes in gene expression. N-3-hydroxydecanoyl-L-Homoserine lactone is a small diffusible signaling molecule secreted by various bacteria. This lactone is produced via lactonolysis from 3-oxodecanoyl-homoserine lactone, altering quorum sensing or contributing to quorum quenching.
Cas No. | 192883-12-8 | SDF | |
别名 | OH-C10-HSL, 3OH-C10-HSL, 3OH-C10-L-HSL | ||
Canonical SMILES | O=C1[C@@H](NC(CC(O)CCCCCCC)=O)CCO1 | ||
分子式 | C14H25NO4 | 分子量 | 271.4 |
溶解度 | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS (pH 7.2) (1:3): 0.2 mg/ml | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.6846 mL | 18.423 mL | 36.846 mL |
5 mM | 0.7369 mL | 3.6846 mL | 7.3692 mL |
10 mM | 0.3685 mL | 1.8423 mL | 3.6846 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
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1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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Candidates of quorum sensing bacteria in activated sludge associated with N-acyl homoserine lactones
Chemosphere 2019 Dec;236:124292.PMID:31310968DOI:10.1016/j.chemosphere.2019.07.023.
This study aimed to explore candidates of microbial groups which is associated with quorum sensing in activated sludge. Activated sludge samples were collected from three wastewater treatment plants (WWTP) to analyze N-acyl homoserine lactone (AHL) by Fourier-transform mass spectrometry (FTMS) and 16S rRNA-based microbial community. Among activated sludge samples taken at 3 WWTPs in different seasons, 2 AHL species of N-3-hydroxyoctanoyl-l-homoserine lactone and N-3-hydroxydecanoyl-L-Homoserine lactone were detected in the range of ranged of 0.1 ng/L to 1.6 ng/L. The detected AHL species were not dependent on treatment systems nor seasons. From microbial community analysis, population abundance of one strain in Verrucomicrobia and two strains in Holophagaceae had high correlation with AHL concentration in activated sludge. Comamonadaceae had also moderately correlated population with AHL concentrations among quorum sensing bacteria reported previously.
Quorum Sensing in a Methane-Oxidizing Bacterium
J Bacteriol 2017 Feb 14;199(5):e00773-16.PMID:27994019DOI:10.1128/JB.00773-16.
Aerobic methanotrophic bacteria use methane as their sole source of carbon and energy and serve as a major sink for the potent greenhouse gas methane in freshwater ecosystems. Dissecting the molecular details of how these organisms interact in the environment may increase our understanding of how they perform this important ecological role. Many bacterial species use quorum sensing (QS) systems to regulate gene expression in a cell density-dependent manner. We have identified a QS system in the genome of Methylobacter tundripaludum, a dominant methane oxidizer in methane enrichments of sediment from Lake Washington (Seattle, WA). We determined that M. tundripaludum produces primarily N-3-hydroxydecanoyl-L-Homoserine lactone (3-OH-C10-HSL) and that its production is governed by a positive feedback loop. We then further characterized this system by determining which genes are regulated by QS in this methane oxidizer using transcriptome sequencing (RNA-seq) and discovered that this system regulates the expression of a putative nonribosomal peptide synthetase biosynthetic gene cluster. Finally, we detected an extracellular factor that is produced by M. tundripaludum in a QS-dependent manner. These results identify and characterize a mode of cellular communication in an aerobic methane-oxidizing bacterium.IMPORTANCE Aerobic methanotrophs are critical for sequestering carbon from the potent greenhouse gas methane in the environment, yet the mechanistic details of chemical interactions in methane-oxidizing bacterial communities are not well understood. Understanding these interactions is important in order to maintain, and potentially optimize, the functional potential of the bacteria that perform this vital ecosystem function. In this work, we identify a quorum sensing system in the aerobic methanotroph Methylobacter tundripaludum and use both chemical and genetic methods to characterize this system at the molecular level.