Mito-Tracker Deep Red 633

Mito-Tracker Deep Red 633 is a novel deep red fluorescent probe that detects mitochondrial membrane potential and specifically stains mitochondria in living cells. By simply incubating with cells, it can be transported passively across the cell membrane and accumulate in biologically active mitochondria, resulting in bright deep red fluorescence.

Mito-Tracker Deep Red 633, with a molecular weight of 586.23, has a maximum excitation wavelength of 622nm and a maximum emission wavelength of 648nm. Please refer to Figure 1 for the excitation and emission spectra and structural formula of Mito-Tracker Deep Red 633.

Figure 1. Excitation and emission spectra of Mito-Tracker Deep Red 633.

Mito-Tracker Deep Red 633 can be used as a mitochondria-specific fluorescent probe. Similar to Rhodamine 123 or JC-1, Mito-Tracker Deep Red 633 staining of mitochondria is dependent on the mitochondrial membrane potential, so the probe can only stain live cells or tissues, not fixed or permeabilized cells or tissues. Please refer to Figure 2 for the staining effect of Mito-Tracker Deep Red 633 on live cells.

Figure 2. The staining effect of Mito-Tracker Deep Red 633 on mitochondria in NRK-52E cells (rat renal tubular epithelial cells). The mitochondria of Mito-Tracker Deep Red 633-stained NRK-52E cells showed magenta (pseudo-color) fluorescence (C). The nuclei were stained with Hoechst 33342. This figure is for reference only, which may vary due to different experimental conditions.

Since the accumulation of Mito-Tracker Deep Red 633 in mitochondria is dependent on the mitochondrial membrane potential, this product can also be used as an indicator probe to detect apoptosis by detecting changes in the mitochondrial membrane potential.

Based on a final working concentration of 50-200nM, each 50µg of this product can be used to prepare approximately 400-1700ml of Mito-Tracker Deep Red 633 staining solution.

Storage Conditions:
Store at -20℃ in the dark, valid for at least one year.

Precautions:
Mito-Tracker Deep Red 633 can only be used for mitochondrial fluorescent labeling of living cells, it cannot be used on fixed cells or tissues, nor can fixation and permeabilization be performed after staining.

For trace amounts of liquid, centrifuge for a few seconds before each use to collect the liquid at the bottom of the tube.

Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.

This product is for R&D only. Not for drug, household, or other uses.

For your safety and health, please wear a lab coat and disposable gloves during the operation.

Mito-Tracker Deep Red 633是一种新型的可以检测线粒体膜电位的深红荧光探针，能够特异性地染色活细胞中的线粒体。只需简单地与细胞孵育，即可通过被动运输穿过细胞膜并聚集在有生物活性的线粒体中，从而可以产生明亮的深红荧光。

Mito-Tracker Deep Red 633，分子量为586.23，最大激发波长为622nm，最大发射波长为648nm。Mito-Tracker Deep Red 633的激发光谱和发射光谱参考图1。

图1. Mito-Tracker Deep Red 633的激发光谱和发射光谱。

Mito-Tracker Deep Red 633可以用作线粒体特异性的荧光探针。和Rhodamine 123或JC-1相似，Mito-Tracker Deep Red 633对于线粒体的染色依赖于线粒体膜电位，因此该探针只能对活的细胞或组织进行染色，不能对固定或通透后的细胞或组织进行染色。使用Mito-Tracker Deep Red 633染色活细胞线粒体的效果参考图2。

图2. Mito-Tracker Deep Red 633 (线粒体深红荧光探针)对于NRK-52E细胞(大鼠肾小管上皮细胞)线粒体的染色效果图。Mito-Tracker Deep Red 633染色的NRK-52E细胞其线粒体呈现洋红色(伪彩)荧光(图C)，洋红色荧光、细胞核蓝色荧光的叠加(merge)效果见图D。其中细胞核使用Hoechst 33342 染色。实际检测效果会因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。

由于Mito-Tracker Deep Red 633在线粒体内的积累依赖于线粒体的膜电位，因此可以作为线粒体膜电位的指示探针，并可以通过检测线粒体膜电位的变化来检测细胞凋亡。

按最终浓度为50-200nM计算，本产品每50µg包装可以配制约400-1700ml Mito-Tracker Deep Red 633染色液。

保存条件：
-20℃避光保存，至少一年有效。

注意事项：
Mito-Tracker Deep Red 633仅可用于活细胞的线粒体荧光标记，不能用于固定后细胞或组织，染色后也不能进行固定、通透等操作。

对于微量的液体，每次使用前先离心数秒钟，使液体充分沉降到管底。

荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

需自备细胞培养板、盖玻片和载玻片等。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

为了您的安全和健康，请穿实验服并戴一次性手套操作。