Menaquinone 9
(Synonyms: 维生素K2(45),MK-9, Vitamin K2(45)) 目录号 : GC44155
A vitamin K2 analog
Cas No.:523-39-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Menaquinone 9 (MK-9) is a vitamin K2 analog that has been found in various bacteria, including E. coli. MK-9 is UV-sensitive and binds to soluble nitrate reductase, a component of the bacterial electron transport chain. In vivo, MK-9 increases plasma prothrombin concentration in vitamin K-deficient rats.
Reference:
[1]. Craciun, A.M., Groenen-van Dooren, M.M., Thijssen, H.H., et al. Induction of prothrombin synthesis by K-vitamins compared in vitamin K-deficient and in brodifacoum-treated rats. Biochim. Biophys. Acta. 1380(1), 75-81 (1998).
[2]. Brito, F., DeMoss, J.A., and Dubourdieu, M. Isolation and identification of menaquinone-9 from purified nitrate reductase of Escherichia coli. Journal of Bacteriology 177(13), 3728-3735 (1995).
| Cas No. | 523-39-7 | SDF | |
| 别名 | 维生素K2(45),MK-9, Vitamin K2(45) | ||
| 化学名 | 2-methyl-3-[(2E,6E,10E,14E,18E,22E,26E,30E)-3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaen-1-yl]-1,4-naphthalenedione | ||
| Canonical SMILES | O=C1C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/C)=C(C)C(C2=CC=CC=C21)=O | ||
| 分子式 | C56H80O2 | 分子量 | 785.2 |
| 溶解度 | 100mg/mL in chloroform | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2736 mL | 6.3678 mL | 12.7356 mL |
| 5 mM | 0.2547 mL | 1.2736 mL | 2.5471 mL |
| 10 mM | 0.1274 mL | 0.6368 mL | 1.2736 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana
Sci Rep 2022 Jul 15;12(1):12114.PMID:35840645DOI:10.1038/s41598-022-16116-3.
A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2T, was isolated from the surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2T had 97.3% and 96.7% 16S rRNA gene sequence similarities with Subtercola boreus K300T and Subtercola lobariae 9583bT, respectively, but formed a distinct phyletic lineage from these two strains. Growth of strain AK-R2A1-2T was observed at 4-25 °C at pH 5.0-8.0. Strain AK-R2A1-2T contained Menaquinone 9 (MK-9) and menaquinone 10 (MK-10) as the predominant respiratory quinones. The major cellular fatty acids were anteiso-C15:0 and summed feature 8 (C18:1ω7c or/and C18:1ω6c), and the polar lipids included diphosphatidylglycerol (DPG) and three unknown aminolipids, AKL2, AKL3, and AKL4. The complete genome of strain AK-R2A1-2T was sequenced to understand the genetic basis of its survival at low temperatures. Multiple copies of cold-associated genes involved in cold-active chaperon, stress response, and DNA repair supported survival of the strain at low temperatures. Strain AK-R2A1-2T was also able to significantly improve rice seedling growth under low temperatures. Thus, this strain represents a novel species of the genus Subtercola, and the proposed name is Subtercola endophyticus sp. nov. The type strain is AK-R2A1-2T (= KCTC 49721T = GDMCC 1.2921T).
Sporichthya brevicatena sp. nov
Int J Syst Bacteriol 1999 Oct;49 Pt 4:1779-84.PMID:10555360DOI:10.1099/00207713-49-4-1779.
A strain was isolated from soil that formed short chains of aerial mycelium dividing into motile spores on an agar medium, but not substrate mycelium. The organism had Menaquinone 9(H8), LL-diaminopimelic acid in the cell wall amino acids and a DNA G + C content of 71 mol%. On the basis of the morphological and chemotaxonomic characteristics and phylogenetic analysis, this strain was classified into the genus Sporichthya. DNA-DNA hybridization revealed that the strain differed from the known species, Sporichthya polymorpha. Therefore, a new species, Sporichthya brevicatena sp. nov., is proposed. The type strain is S. brevicatena YU720-21T (= IFO 16195T).