Maslinic Acid
(Synonyms: 山楂酸; Crategolic acid; 2α-Hydroxyoleanolic acid) 目录号 : GC17632
A biologically active triterpene
Cas No.:4373-41-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
RAW 264.7 cells are seeded in 96-well culture plates at a density of 1×105 cells/mL and after incubation for 24 h, are treated with OPE1 (300 μg/mL or 400 μg/mL), OPE2 (20 μg/mL or 40 μg/mL), or Maslinic acid (10 μM or 20 μM) , as well as with/without LPS at the same time. Cell viability i determined using the WST-1 reagent. Briefly, WST-1 reagent (10 μL) is added to each well and incubated for 1h in a humidified incubator. The absorbance of the samples measured at 450 nm (reference wavelength is 750 nm). Viability is expressed as a percentage of the absorbance measured in LPS-treated cells[1]. |
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Animal experiment: |
Mice[1]Five-week-old male Balb/c mice (19-21 g) are housed in a conventional condition and provided with the free access to standard rodent chow and water. Edema is induced by intraplantar injection of 100 μL 1% carrageenan into the hind left paw. Maslinic acid is tested initially at a dose of 200 mg/kg, orally administered 60 min before and after carrageenan injection. Paw thickness is measured using electronic digital calipers, 2, 3, and 4 h following carrageenan treatment. Mice are sacrificed by carbon dioxide inhalation 4 h after carrageenan injection[1]. |
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References: [1]. Fukumitsu S, et al. Anti-inflammatory and anti-arthritic effects of pentacyclic triterpenoids maslinic acid through NF-κB inactivation. Mol Nutr Food Res. 2016 Feb;60(2):399-409. |
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Maslinic acid is a pentacyclic triterpene existed in the protective wax-like coating of the leaves and fruit of Olea europaea L [1]. Maslinic acid is an inhibitor of NO, H2O2, IL-6 and TNF-α.
In HT29 colon-cancer cells, treatment with maslinic acid significantly inhibited cell proliferation in a dose-dependent manner with the IC50 value of 28.8 ± 0.9 μg/mL. Maslinic acid inhibited the expression of Bcl-2 whilst increasing that of Bax. Maslinic acid stimulated the release of mitochondrial cytochrome-c and ativated aspase-9 and caspase-3 [1]. Maslinic acid inhibited the activity of serine proteases, key enzymes necessary for the spread of HIV within an individual's body. Maslinic acid decreased the cytopathic effect and p24 antigen levels in MT2 cells [2].
In KK-Ay mice, an animal model of genetic type-2 diabetes, oral administration of MA (10 mg/kg) reduced the blood glucose levels. Administration of maslinic acid (10 mg/kg and 30 mg/kg) for 2 weeks significantly reduced the blood glucose levels [3]. Pretreatment with MA attenuated ischemia-induced translocation of NF-κB p65 subunit to the nucleus [4].
References:
[1] Reyes-Zurita F J, Rufino-Palomares E E, Lupiáez J A, et al. Maslinic acid, a natural triterpene from Olea europaea L., induces apoptosis in HT29 human colon-cancer cells via the mitochondrial apoptotic pathway[J]. Cancer letters, 2009, 273(1): 44-54.
[2] García, A. Compound from olive-pomace oil inhibits HIV spread. 070709111536, 1-1 (2007).
[3] Liu J, Sun H, Duan W, et al. Maslinic acid reduces blood glucose in KK-Ay mice[J]. Biological and Pharmaceutical Bulletin, 2007, 30(11): 2075-2078.
[4] Guan T, Qian Y, Tang X, et al. Maslinic acid, a natural inhibitor of glycogen phosphorylase, reduces cerebral ischemic injury in hyperglycemic rats by GLT‐1 up‐regulation[J]. Journal of neuroscience research, 2011, 89(11): 1829-1839.
| Cas No. | 4373-41-5 | SDF | |
| 别名 | 山楂酸; Crategolic acid; 2α-Hydroxyoleanolic acid | ||
| 化学名 | 2α,3β-dihydroxy-olean-12-en-28-oic acid | ||
| Canonical SMILES | CC1(C)CC[C@]2(C(O)=O)CC[C@@]3(C)[C@]4(C)CCC5C(C)(C)[C@@H](O)[C@H](O)C[C@]5(C)[C@@]4([H])CC=C3[C@@]2([H])C1 | ||
| 分子式 | C30H48O4 | 分子量 | 472.7 |
| 溶解度 | ≤0.5mg/ml in ethanol;20mg/ml in DMSO;15mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1155 mL | 10.5775 mL | 21.1551 mL |
| 5 mM | 0.4231 mL | 2.1155 mL | 4.231 mL |
| 10 mM | 0.2116 mL | 1.0578 mL | 2.1155 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。