Leukotriene D4 methyl ester
目录号 : GC44055A more lipid soluble formulation of LTD4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Leukotriene D4 (LTD4) is one of the constituents of slow-reacting substance of anaphylaxis (SRS-A) produced by the metabolism of LTC4 by γ-glutamyl transpeptidase.[1],[2] LTD4-induced bronchoconstriction and enhanced vascular permeability contribute to the pathogenesis of asthma and acute hypersensitivity.[3],[4] LTD4 is several hundred fold more potent than LTC4 at the cysteinyl-leukotriene 1 (CysLT1) receptor, but they exhibit approximately equal affinity at the CysLT2 receptor.[5],[6] LTD4 methyl ester is a more lipid soluble form of LTD4. The biological activity of LTD4 methyl ester has not been reported.
Reference:
[1]. Örning, L., Hammarström, S., and Samuelsson, B. Leukotriene D: A slow reacting substance from rat basophilic leukemia cells. Proceedings of the National Academy of Sciences of the United States of America 77, 2014-2017 (1980).
[2]. Hammarström, S., Örning, L., and Bernström, K. Metabolism of leukotrienes. Molecular and Cellular Biochemistry 69, 7-16 (1985).
[3]. Hedqvist, P., Dahlén, S.E., Gustafsson, L., et al. Biological profile of leukotrienes C4 and D4. Acta Physiologica Scandinavica 110, 331-333 (1980).
[4]. Samuelsson, B., Dahlén, S.E., Lindgren, J.Å., et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237, 1171-1176 (1987).
[5]. Lynch, K.R., O'Neill, G.P., Liu, Q., et al. Characterization of the human cysteinyl leukotriene CysLT1 receptor. Nature 399(6738), 789-793 (1999).
[6]. Heise, C.E., O'Dowd, B.F., Figueroa, D.J., et al. Characterization of the human cysteinyl leukotriene 2 receptor. J. Biol. Chem. 275(39), 30531-30536 (2000).
| Cas No. | SDF | ||
| 化学名 | 5S-hydroxy-6R-(S-cysteinylglycinyl)-7E,9E,11Z,14Z-eicosatetraenoic acid, methyl ester | ||
| Canonical SMILES | CCCCC/C=C\C/C=C\C=C\C=C\[C@@H](SC[C@H](N)C(NCC(O)=O)=O)[C@@H](O)CCCC(OC)=O | ||
| 分子式 | C26H42N2O6S | 分子量 | 510.7 |
| 溶解度 | 20mg/mL in DMSO, or in DMF | 储存条件 | Store at -80°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9581 mL | 9.7905 mL | 19.581 mL |
| 5 mM | 0.3916 mL | 1.9581 mL | 3.9162 mL |
| 10 mM | 0.1958 mL | 0.979 mL | 1.9581 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。