Leucomalachite green
(Synonyms: 隐色孔雀绿) 目录号 : GC60987
Leucomalachite green is a chief metabolite of malachite green, a triphenylmethane dye. It is one of the most commonly used reagents for presumptive blood test and gives a characteristic green color in the presence of blood.
Cas No.:129-73-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Leucomalachite green is a chief metabolite of malachite green, a triphenylmethane dye. It is one of the most commonly used reagents for presumptive blood test and gives a characteristic green color in the presence of blood.
| Cas No. | 129-73-7 | SDF | |
| 别名 | 隐色孔雀绿 | ||
| Canonical SMILES | CN(C)C1=CC=C(C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3)C=C1 | ||
| 分子式 | C23H26N2 | 分子量 | 330.47 |
| 溶解度 | DMSO: 100 mg/mL (302.60 mM) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.026 mL | 15.13 mL | 30.2599 mL |
| 5 mM | 0.6052 mL | 3.026 mL | 6.052 mL |
| 10 mM | 0.3026 mL | 1.513 mL | 3.026 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Malachite green and Leucomalachite green in fish: a global systematic review and meta-analysis
Environ Sci Pollut Res Int 2023 Mar 15.PMID:36920616DOI:10.1007/s11356-023-26372-z.
Malachite green (MG), an antibiotic with antifungal activity, is illegally used in aquaculture. Given that this chemical is teratogenic and mutagenic, abstinence from intake seems to be a need for public safety. The goal of this systematic review and meta-analysis was to determine the global contamination of fishes by MG and its reduced metabolite, Leucomalachite green (LMG), in a number of marine and farmed fish species. For literature published prior to January 2022, several databases (Web of Science, PubMed, and Scopus) were investigated. In total, 20 publications (10 countries, 724 samples) achieved the criteria for inclusion. The overall average MG and LMG concentrations were 0.48 (95% CI: 0.47, 0.49 µg kg-1) and 0.59 (95% CI: 0.39, 0.79 µg/kg-1), respectively. Eel (M. albus) 15.50 (95% CI: (14.39, 45.39 µg kg-1) and eel (A. anguilla) 4.46 (95% CI: 1.23, 7.69 µg kg-1) had the greatest contamination of MG and LMG, according to the effect size, respectively. Warm-water fish had a concentration of 2.591 (95% CI: 2.25, 2.93 µg kg-1) while cold-water fish had a concentration of 1.55 (95% CI: 0.25, 2.84 µg kg-1). Fish containing medium-fat level of 1.86 (95% CI: 1.27, 2.44 µg kg-1) and high-fat content of 1.10 (95% CI: 0.93, 1.26 µg kg-1) had accumulate MG and LMG in their tissues, respectively. As a result, total MG observed in countries including China, Iran, and the Netherlands was higher than authorized (2 µg kg-1). The toxicity of MG and LMG demands more monitoring, especially in countries where these chemicals' residues are significant.
Selection and characterization of bispecific aptamers against malachite green and Leucomalachite green
Anal Biochem 2022 Dec 1;658:114849.PMID:36150472DOI:10.1016/j.ab.2022.114849.
In order to develop multi-residues rapid detection, the bispecific aptamers against malachite green (MG) and Leucomalachite green (LMG) were isolated by the capture systematic evolution of ligands by exponential enrichment (Capture-SELEX). After thirteen rounds of selection, the enriched ssDNA pools were sent for high-throughput sequencing. Nine aptamer candidates (A1-A9) were picked out to test their specificity by gold nanoparticles (AuNPs) colorimetric assay. Three aptamers (A2, A3, A5) with good selectivity were truncated to verify their affinity by fluorescence assay. Finally, three truncated aptamers (A2-a, A3-a, A5-a) with bispecificity and high affinity were identified. For LMG, the dissociation constant (Kd) of them were 8.4 ± 0.8 nM, 8.2 ± 1.2 nM, and 13.7 ± 1.4 nM, respectively. For MG, Kd of them were 3.4 ± 0.3 μM, 2.3 ± 0.2 μM, 3.0 ± 0.2 μM. Among them, A3-a is the best. Our work will provide novel probes for the development of multi-residues rapid detection as well as opportunities for multiple target aptamer discovery.
Genotoxicity of malachite green and Leucomalachite green in female Big Blue B6C3F1 mice
Mutat Res 2004 Jul 11;561(1-2):127-38.PMID:15238237DOI:10.1016/j.mrgentox.2004.04.003.
Malachite green, a triphenylmethane dye used in aquaculture as an antifungal agent, is rapidly reduced in vivo to Leucomalachite green. Previous studies in which female B6C3F1 mice were fed malachite green produced relatively high levels of liver DNA adducts after 28 days, but no significant induction of liver tumors was detected in a 2-year feeding study. Comparable experiments conducted with Leucomalachite green resulted in relatively low levels of liver DNA adducts but a dose-responsive induction of liver tumors. In the present study, we fed transgenic female Big Blue B6C3F1 mice with 450 ppm malachite green and 204 and 408 ppm Leucomalachite green (the high doses used in the tumor bioassays) and evaluated genotoxicity after 4 and 16 weeks of treatment. Neither malachite green nor Leucomalachite green increased the peripheral blood micronucleus frequency or Hprt lymphocyte mutant frequency at either time point; however, the 16-week treatment with 408 ppm Leucomalachite green did increase the liver cII mutant frequency. Similar increases in liver cII mutant frequency were not seen in the mice treated for 16 weeks with malachite green or in female Big Blue rats treated with a comparable dose of Leucomalachite green for 16 weeks in a previous study [Mutat. Res. 547 (2004) 5]. These results indicate that Leucomalachite green is an in vivo mutagen in transgenic female mouse liver and that the mutagenicities of malachite green and Leucomalachite green correlate with their tumorigenicities in mice and rats. The lack of increased micronucleus frequencies and lymphocyte Hprt mutants in female mice treated with Leucomalachite green suggests that its genotoxicity is targeted to the tissue at risk for tumor induction.
Application of the margin of exposure (MOE) approach to substances in food that are genotoxic and carcinogenic. Example: Leucomalachite green
Food Chem Toxicol 2010 Jan;48 Suppl 1:S75-80.PMID:19786059DOI:10.1016/j.fct.2009.09.025.
Leucomalachite green (LMG) is mutagenic and produces DNA-adducts in vivo, and is carcinogenic in rodent bioassays. Dose-response modelling of the data for hepatocellular adenomas and carcinomas in female mice gave a BMDL10 of 20 mg/kg-bw/day. Limited data are available on the concentrations present in fish for human consumption. Human exposure estimates assumed that all consumed fish is contaminated with LMG. The calculated MoEs were 4,000,000 and 400,000 respectively for average and high exposure estimates.
Carcinogenicity of malachite green chloride and Leucomalachite green in B6C3F1 mice and F344 rats
Food Chem Toxicol 2006 Aug;44(8):1204-12.PMID:16554117DOI:10.1016/j.fct.2006.01.016.
Malachite green is a triphenylmethane dye used in the fish industry as an anti-fungal agent. Leucomalachite green is formed by the metabolic reduction of malachite green and persists in the tissues of exposed fish. In this study, we examined the carcinogenicity of malachite green chloride and Leucomalachite green. Female F344 rats (48 per group) were fed diets containing 0, 100, 300, or 600 ppm malachite green chloride for 104 weeks, at which time the extent of tumorigenesis was assessed. Additional groups of 48 female and 48 male F344 rats were fed diets containing 0, 91, 272, or 543 ppm Leucomalachite green for 104 weeks. Groups of 48 female B6C3F1 mice were fed diets containing 0, 100, 225, or 450 ppm malachite green chloride or 0, 91, 204, or 408 ppm Leucomalachite green for 104 weeks. For each of the exposures, food consumption in the treatment groups was similar to the controls. Rats fed malachite green chloride or Leucomalachite green had dose-dependent reductions in body weight; in mice, there were no consistent effects upon body weights with either compound. Female rats exposed to malachite green chloride had increased incidences of thyroid gland follicular cell adenoma or carcinoma and hepatocellular adenoma, and a dose-related increasing trend in mammary gland carcinoma. Female rats fed malachite green chloride and female and male rats fed Leucomalachite green had a dose-related decreasing trend in the incidence of mononuclear cell leukemia. In male rats fed Leucomalachite green there was a decreasing trend in pituitary gland adenoma and an increasing trend in interstitial cell adenoma of the testis. There were no treatment-related neoplasms in female B6C3F1 mice fed malachite green chloride. Female mice fed Leucomalachite green had a dose-related increasing trend in the incidence of hepatocellular adenoma or carcinoma, with the incidence being significant in the highest dose group.